表明

The results show that the optimal value of CoCl2 concentration is 0.2 mol/L and the maximum adsorbed amount is 19.674 mg/g onto this adsorbent. The adsorption capacity of phosphine onto CoCl2-modified ACF decreases with the increase of temperatures. The maximum absorbed amounts are 19.674 mg/g at 298 K, 13.537 mg/g at 313 K and 11.087 mg/g at 328 K, respectively. It is found that the Freundlich equation is more suitable for the description of phosphine adsorption process than the Langmuir equation. The isosteric heat of adsorption decreases with the increase of the surface loading on CoCl2-modified ACF, which means that CoCl2-modified ACF adsorbent has an energetically heterogeneous surface. Meanwhile, adsorptive phosphine removal performance may be a dominant of physical adsorption when the heat of adsorption is 16-24 kJ/mol, the CoCl2-modified ACF adsorbent will be one of the candidates for tail gas purification of airtight calcium-carbide furnace and recycle of phosphine.

研究结果表明：浸渍液浓度最佳值为0.2 mol/L，此改性ACF对PH3的饱和吸附量为19.674 mg/g；PH3在CoCl2改性ACF上的吸附量随温度升高而迅速降低，在298，313和328 K时PH3的饱和吸附量分别为19.674，13.537和11.087 mg/g；Freundlich吸附等温方程较好地模拟了PH3在改性ACF上的等温吸附；PH3气体在改性ACF上的等量吸附热随吸附量的增大而减小，表明改性ACF吸附剂表面能量的不均匀性；吸附热在16~24 kJ/mol范围内，过程为物理吸附，有利于密闭电石炉尾气的净化。

Results: Within-run CV 、day-to-day CV and total CV were 1.33%、1.42% and 2.01% respectively by iterant test; average recovery rate was 96.6%; anticoag-ulant、septic、uric acid and bilirubin under a certain concentration also did not interfere with the glucose re-sult in this method. Compared with HK method, the pertinence was Y =1.038X-1.172,r =0.994. The linearity studies showed the method was linear up to 20mmol/L.

结果： 重复性试验批内 CV 值为1.33%，批间CV 值为1.42%，总 CV 值为2.01%；回收试验中回收样品I、Ⅱ、Ⅲ的回收率分别为105%，93.5%和91.4%，平均为96.6%；干扰试验表明一般浓度的抗凝剂、防腐剂、尿酸、胆红素对葡萄糖的测定不产生干扰；比较试验，与HK法比较其相关性为Y =1.038X-1.172，r =0.994；线性范围试验表明葡萄糖浓度在20 mmol/L以下呈现良好的线性。

Results: Within-run CV 、day-to-day CV and total CV were 1.33%、1.42% and 2.01% respectively by iterant test; average recovery rate was 96.6%; anticoag-ulant、septic、uric acid and bilirubin under a certain concentration also did not interfere with the glucose re-sult in this method.

结果： 重复性试验批内 CV 值为1.33%，批间CV 值为1.42%，总 CV 值为2.01%；回收试验中回收样品I、Ⅱ、Ⅲ的回收率分别为105%，93.5%和91.4%，平均为96.6%；干扰试验表明一般浓度的抗凝剂、防腐剂、尿酸、胆红素对葡萄糖的测定不产生干扰；比较试验，与HK法比较其相关性为Y =1.038X-1.172，r =0.994；线性范围试验表明葡萄糖浓度在20 mmol/L以下呈现良好的线性。

A full-length cDNA encoding sucrose: sucrose 1-fructosyltransferase from Lactuca sativa was inserted into pCAMBIA1300-als under the control of the CaMV 35S promoter. This plasmid was used for Agrobacteriummediated tobacco leaf disc transformation. Transgenic plants were analyzed by PCR and Southern blotting. 1-SST gene expression was confirmed by RT-PCR.

将从莴苣中克隆的1-SST基因重组到pCAMBIA1300-als中，构建了在CaMV 35S启动子调控下的植物表达载体，利用农杆菌介导的叶盘转化法将1-SST基因导入烟草中，PCR和Southern杂交检测表明获得了转基因植株，RT-PCR结果表明该基因在烟草中正常表达。

The simulation result,which is obtained by applying the method to control the temperature of latices polyreactor,shows static error is effectively reduced and the robustness of the new approach in controlling temperature shock in the process of p...

将该控制器用于胶乳聚合反应釜的温度控制，仿真结果表明该控制器可以有效地消除静态误差，并对聚合反应生产过程中的温度骤变有很好的鲁棒性，实际应用效果也表明了该方法的优越性。

Compared with the conventionalemulsion polymerization system in the presence of sodium dodecyl benzene sulfonate, the latices prepared by emulsifier-free emulsion polymerization have much more narrow distribution of latex particle sizes (0.2um in diameter), better resistance to electrolytes, pH, freeze-thaw, and better mechanical stability. Not only the tensile strength of the copolymers prepared by this method is obviously increased, but also the water resistance and adhesion capacity are significantly enhanced. On the other hand, the TG results indicate the copolymer has lower thermal stability than conventional copolymer.

实验结果表明，与传统乳液聚合体系相比，所获得的无皂乳液的乳胶粒子大小均一，粒径在0.20um左右；乳液具有优异的稳定性，不受酸碱度、电解质和低温冷冻的影响；附着力和粘接强度明显提高；成膜后显示较高的耐水性；但TG表明乳胶膜热稳定性有所下降。

The results show that the chlorite jade is in dark green, semi-translucent to opaque, and lepidoblastic texture, with hardness of 2.5, refractive index 1.58 and density 2.60 g/cm^3. It has homogeneous texture and less fissures, and no fluorescence under ultraviolet fluorescence lamp. XRD analysis shows that the mineral composition of the chlorite jade is mainly chlorite.

结果表明，该绿泥石玉呈深绿色、微透明－不透明集合体，具鳞片变晶结构，裂隙少，质地均匀，在紫外荧光灯下不发荧光，摩氏硬度为2.5，折射率为1.58，密度为2.60g/立方公分。X射线衍射分析结果表明，该绿泥石玉的物相为绿泥石。

Our results demonstrate that (1) the lipogenic tumor markers CDK4 and MDM2 can be used as surrogate immunohistochemical markers for the diagnosis of malignant lipomatous tumors with high sensitivity;(2) approximately 26% of retroperitoneal/thigh UHGPS cases that were positive for PPAR-γ, CDK4, or MDM2 by immunohistochemistry showed characteristic CDK4 and MDM2 gene amplification, suggesting that a subset of UHGPS cases represent DDL despite lacking histologic evidence of lipoblasts.

我们的结果表明：（1）脂肪源性肿瘤标记CDK4和MDM2可以作为诊断恶性脂肪肿瘤的免疫标记物，且敏感性高；（2）腹膜后/大腿UHGPS免疫表达PPAR-γ， CDK4或MDM2的病例中，约有26%例显示特征性的CDK4和MDM2基因扩增，这表明部分UHGPS病例表现为DDL，虽然组织学上找不到脂母细胞。

The results showed that expression plasmid pET22b-lysB was constructed successfully. Highly purified recombination protein was obtained 33.2 mg from 1 L LB culture medium. A screening for His-LysB activity on esterase and lipase substrates confirmed the lipolytic activity. With p-nitrophenyl butyrate as substrate, the thermal stability of the enzyme was poor when the temperature was above 30oC. The enzyme exhibited higher stability at pH 5.0–9.5. The optimum temperature and pH for the lipolytic activity of His-LysB were 23oC and 7.5 respectively. Under the optimum conditions, the specific activity of His-LysB was 1.3 U/mg. Zn2+, Cu2+, Mg2+, Mn2+and phenylmethane sulfonyl fruoride severely inhibited the lipolytic activity of His-LysB.

结果表明：成功构建了pET22b-lysB表达载体，并从1 L的LB培养物中获得了33.2 mg高纯度重组蛋白；His-LysB具有分解脂肪的能力，属于脂肪酶；生物化学特性分析表明：丁酸对硝基苯为水解底物，His-Lys热稳定性不佳，30℃以下比较稳定，随着温度的升高，稳定性逐渐降低；该蛋白具有较高的pH值适应性，pH 5.0~9.5范围内稳定性较高；在23℃和pH 7.5时酶活力最高，其比酶活为1.3 U/mg；金属离子Zn2+、Cu2+、Mg2+、Mn2+和苯甲基磺酰氟抑制剂对酶活具有强烈的抑制作用。

Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰，直径约1mm，成斑时间12h；从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号： AY639599)，又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因，表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli， E.coli)中表达获得了47kDa的清晰表达带，在1h 时开始产生蛋白并有逐步上升的趋势； Western blot 也在47kDa处得到一条清晰的条带；可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的，该蛋白的产生明显地抑制了宿主的生长速度；噬菌体PEP氨基酸序列之间的同源性比较表明，噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。