表明

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段，应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段；在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法；检测方法的特异性试验结果表明，该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应；检测方法的敏感性试验结果表明，通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg；对20份临床样品检测中，有10份为AIV阳性，其中有5份为H5亚型病毒阳性。

The conversion boundary of self-incompatibility was researched by sowing by stage. Sunshine of day and temperature was studied critically by investigating leafage and researching traits indoors. The results showed that the daily minimum temperature was the principal factor of affinity conversion and the affinity conversion boundary was 16.5~18.1℃.HE97 was primarily regard as self-incompatible line in this research. The method was by inbreeding and sib HE97, Half the silk of HE97 and Zheng58. Inbreeding and cross have been done simultaneously. The extending condition of pollen tube was observed by dying the silk after pollinating 4 hours. The individuals with pollen tube were observed having seeds, while the individuals without pollen tube were observed having few seeds or no seeds. So this study regard HE97 as sporophytic self-incompatibility .The planting ration of female parent HE97 and male parent 87-1 was 4:1.The nature cross-pollination was studied by the theory of endosperm xenity, the study show that the setting percentage of HE97 was 625%, in which the seed in self was 70% and the seed in hybrid was 30%. This was consistent with the study of silk anatomy structure, then come to the same conclusion: compatible pollen germination and fertilization can stimulate the self-incompatible pollen germination and fertilization in the same corn cob.

通过分期播种，进行了光温敏自交不亲和材料HE97亲和性转换区间的研究，依据田间跟踪调查叶龄及室内考种研究了HE97亲和性转换区间的光温临界值，结果表明：1日最低温度是影响HE97自交不亲和性的主要原因，亲和性转换区间在16.5℃～18.1℃之间。2通过HE97自交和姊妹交以及将HE97与郑58作为双亲，各自的花丝一分为二，分别正反交，授粉后4个小时分别取其花丝进行固定、染色，观察花粉管的伸长情况，根据结实率与花粉管伸长的对应关系，结果发现观察到花粉管的对应植株收获到了种子，而没有观察到花粉管的对应植株结实率极低或没有结实，本研究把HE97初步划为孢子体自交不亲和类型。3以HE97为母本，87-1为父本，按4母：1父行比，通过花粉直感效应研究天然异交率，结果母本HE97结实良好(62%)，其中自交粒占70%，异交粒占30%，结合花丝切片观察表明，亲和花粉的萌发及受精会导致落在同一果穗柱头上不亲和花粉的萌发不再受抑制。

The molecular weight of broad bean vicilin and legumin measured by gel filtration chromatography were 135000 and 351000, DSC showed that their denature temperatures were 72℃ and 87℃ respectively. The legumin had a lowest solubility at pH 4. 5-5. 0 whereas the vicilin at pH5. 5. Vicilin and legumin were all possessed low levels of α-helical and high β-sheet secondary structure fractions. The total content of α-helical,β-sheet and β-turn fraction of vicilin and legumin reached to 90% and 78% separately showed that they had a high ordered structure in solution.

对两种组分的凝胶层析表明7S和11S的相对分子质量分别为135000和351000；DSC分析显示其热变性温度分别为72℃和87℃；溶解度分析表明：11S球蛋白在pH4.5-5.0之间，7S球蛋白在pH5.5时具有最低的溶解度；圆二色性分析结果显示7S和11S球蛋白的β-折叠结构含量分别高达40.5％和38.1％，证明二者都属于β-折叠型蛋白质，二者的α-螺旋、β-折叠和β-转角结构的含量总和分别达到了90％和78％，从而说明它们在溶液中的结构是高度有序的。

Simulation results demonstrate that it can lead to the convergence of the queue length to the desired value quickly and maintain the oscillation small.

仿真结果表明该方法可以使队列长度快速收敛到设定值，同时使队列震荡最小，结果也表明在网络条件变化的情况下，该算法优于模糊控制和变结构控制。

Second, with regard to unlimitedness and limitedness of the material world, the philosophical outlook of the universe differs from that of the natural science....

在决定论和非决定论问题上，量子力学的统计特征并不表明世界在本性上是统计性的或非决定论的，而只表明客体间相互作用是必然和偶然的统一。

He even reminds me of the look he gave me as he was going up the elevator shaft. He dwells on that lingeringly, as though to imply that everything were contained in that last moment, as though, if he had the power to alter things, he would never have put foot outside the elevator.

他甚至又令我想起上电梯前他曾那样瞥了我一眼，他反反复复提起这一点，像是要表明一切都包含在这最后一秒钟里，像是要表明如果他有力量改变局面，他就绝不会跨出电梯。

Moreover, in our experiments, we compare thequantification of other features, such as pleural indentation, lobulation sign et al, with thequalitative classification provided by radiologists. The results indicate that mostquantification of the features can obtain consistency higher than 87%.

针对毛刺征提出了一种基于边界法线-梯度正交性的毛刺量化方法，并与已有的毛刺量化方法进行了比较评估，分析表明该方法的量化结果与真实的毛刺水平具有更高的一致性；实验结果还表明分叶征、胸膜凹陷征等其它特征的量化分级大部分具有87％以上的正确率。

Immunohistochemical localization of inhibin α-subunit in normal mature rats was carried out by using Strept Avidin-Biotin-Peroxidase Complex .The results showed that there was positive immunoreactivity of the McAb to inhibinα-subunit in heart ,kidney , spleen , lymph ,adrenal gland and pancreas cells. It indicated that inhibinα-subunit existed in the above tissues , But no positive sites were seen in hypothalamus, pituitary, lung and liver, that means they have no inhibinα-subunit. Compared with the ovariectomized rats, the hypothalamus and pituitary of the rats injected with inhibin-α fragment（1～32）showed an positive reaction, suggesting that inhibin α-subunit could pass through the blood-brain barrier .

用链霉亲和素-生物素-过氧化物酶(Strept Avidin-Biotin-Peroxidase Complex， SABC)免疫组织化学方法，以抑制素α亚基单克隆抗体对正常雌性性成熟大鼠各组织的抑制素α亚基进行定位观察，结果显示：⑴大鼠心脏、肾、脾、淋巴、肾上腺、胰腺均呈阳性反应，表明存在抑制素α亚基；下丘脑、垂体、肝脏和肺组织呈阴性反应，表明其不存在抑制素α亚基；⑵与去卵巢大鼠相比，外源注射抑制素α（1～32）片段的大鼠其下丘脑和垂体出现阳性反应，说明抑制素α亚基能通过血脑屏障。

The results show that the moisture from the Bay of Bengal, the southwest jet on south china sea,the east wind on the low layer and the generation and development of the depression provide macro condition for the generation of the heavy snowstorm,besides which the background physical f...

结果表明，来自孟加拉湾、南海的西南急流以及低层偏东风水汽输送和低值系统的发生发展，为暴雪产生提供了有利的宏观条件；物理量的进一步分析表明了此次暴雪和雾凇天气均具有良好背景场。

This showed that capacity of water-holding in biological soil crusts were obvious improve,and effected precipitation permeate or water second distribute process.The study results of"fertile islands"formation mechanism in biological crusts showed that biological crusts layer cover could increased nutrients content for below sand soil through precipitation permeate washing,and it promoted plants assimilate more nutriment.The matter import ways of biological soil crusts fertility island had many ways,including the effection of living macrobiotics metabolism in crusts levels,wind-sand flow of near surface,dust deposition and plants decomposition course,etc.Source of particle matter in biological soil crusts was wind-sand flow of near surface,and not dust deposition.Biological crusts layer cover had preserved effection for nature water content in 0-5cm soil layer,compared with the sand of no biological crusts layer,effecting precipitation permeate,and could not obvious restrained from the water evaporate process indoor experiment.

生物结皮层肥岛形成机制的研究结果表明：生物结皮层覆盖能够通过降水淋溶作用而增加下层土壤养分的含量，进而促进植物对养分的吸收；生物结皮层养分输入有结皮生物代谢、降尘、风沙流和植物分解矿化作用等多(来源：A73e3B3eC论文网www.abclunwen.com)种途径，但生物结皮层的颗粒物质来源主要是近地表风沙流，而不是大气降尘；生物结皮层覆盖对0-5cm土层自然含水量有一定的保护作用，并影响降水向深层渗透，不能有效的阻止表层水分的蒸发进程；研究表明采取人工促进技术能够在流动沙丘上形成生物结皮层，实现流动沙地的快速治理。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。