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Rechallenge was performed 60 days after first tumor inoculation, all mice showed complete protection, indicating that the DC-gp100 induced specific anti-melanoma memory CD8+CTL.

为了验证NV抗原表位可以降低gp100特异的CD8+T 细胞活化阈值,我们先将NV抗原肽负载DC,接着再分别负载不同浓度梯度的gp100抗原肽,以此免疫小鼠,7天后以淋巴细胞增殖实验判断gp100特异的CD8+T 细胞活化阈值。

To use phage display construction antibody library, trabant peptide library, protein mutant library and cDNA library, thus generally using this technology to screen single chain antibody, le...

利用噬菌体表面展示技术可以构建抗体库、随机肽库、蛋白质突变体库和cDNA文库,从而广泛用于单链抗体的筛选、先导化合物的筛选、疫苗的研制等,尤其是在单链抗体、模拟抗原表位的筛选及建立食品安全快速检测体系方面,具有广阔的应用前景。

Methods The frequency of HBV specific CTL cells m the peripheral blood from 40 patients infected with HBV were quantified by flow cytometry using one HLA-A * 0201-HbcAgl8-27 peptide pentamer complex.

使用HBV抗原表位肽五聚体(HBcAg18-27)对急性和慢性乙型肝炎感染患者外周血染色后,用流式细胞仪检测HBV特异性CTL细胞。

Our study suggested that it is available to get human anti-toxin with the linear neutralizing epitope as immune target.

表明以线性中和表位作为靶标,制备人源抗毒素的技术路线是可行的。

Pneumoniae, indicating that some of chlamydial surface molecules share antigenic epitopes.

这些结果都说明了一些衣原体表面分子可以有共同的抗原表位

Methods: Encapsulate synthesized MG7 mimotope peptide into nanoemulsion using magnetic and ultrasonic technique. Dialyse method was used to sterilize, HPLC to determine encapsulation efficiency.

人工合成胃癌MG7-Ag的模拟表位多肽,用磁力超声法将其包封于纳米乳剂中,通过透析纯化,HPLC检测其包封效率。

Since haptens do not occupy the entire available paratope we hypothesized that hapten-specific antibodies, as they develop naturally or in the laboratory, have an imprint of the carrier protein they were once selected on.

由于半抗原并没有占据所有可利用的表位,因而我们设想,特异的半抗原抗体在天然形成或在实验室合成时,肯定具有一些曾经被用来对其进行筛选的载体蛋白的某些特征。

Methods: Based on B-cell epitope 82~93, a multiple antigenic peptide system was synthesized as immunogen and C-57BL/6 mice were divided into 3 groups randomly. MAP group (n=5) referred to that C-57BL/6 mice were immunised with the immunogen and the polyclonal anti-serum was produced. The polyclonal antibody was identified by using an ELISA assay.

将C-57BL/6小鼠随机分为对照组、MAP组、人参总皂苷组,MAP组在预测MAGE-12的B细胞表位序列为QSDEGSSNEEQE(82-93)的基础上,采用4分枝多重抗原肽结构设计合成抗原肽,免疫C-57BL/6小鼠,产生多克隆抗体,应用ELISA实验检测多克隆抗体是否为人的抗原肽的特异性抗体。

The isotypes of the two monoclonal antibodies were identified as IgG1, and their directed epitope located in PreS1 (34-47) identified by Western blotting analysis.

并利用上述8个序列重叠的PreS1融合蛋白,通过免疫印迹的方法鉴定了它们的作用表位位于PreS1(34-47)内。

The location in virions of the epitopes recognized by the five scFv clones was determined by immunoelectron microscopy.

并通过免疫胶体金电镜分析,对5种单链抗体对应在病毒粒子上的表位进行了定位。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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