表位

The heterologous protein fragments are so-called CTL epitope, which is the linear fragment or the space conformation structure recognized specifically by immune cell and plays a key role in CTLs activation. It is the elementary event of inducing immune responses and it decides CTL specific cytotoxic effects, therefore, selection of antigen epitope makes the pivotal step to identification of specific antibodies.

抗原肽是抗原中能被免疫细胞特异性识别的线性片段或空间构象性结构，是引起免疫应答和免疫反应的基本单位，在机体免疫中发挥关键的作用，抗原表位的选择也是能否获得特异性抗体的关键。

In order to detect the distribution of silica-binding proteins in rice and other graminaceous plants, specific polyclonal antibodies against a silica-binding protein from rice, namely SBP117, are successfully raised by synthesized peptides Which are conjugated with Keyhole Limpet Hemocyanin and used as antigens to immunize rabbits.

为了检测硅结合蛋白在水稻和禾本科植物体内的分布，根据硅结合蛋白(silica-binding protein 117， SBP117)的氨基酸保守片段和天然抗原表位，合成了SBP117的两个肽段做抗原，与匙血蓝蛋白载体偶联后免疫兔子，获得了抗SBP117的专一性抗体。

That synergistic action was not performed through pathway of IL-2 secretion, and need no cross-link between DAF and CD3.Hydrophilicity, solvent accessibility, secondary structure and B cell epitopes of DAF were predicted by protein surface analysis and molecular model of SCR3 and SCR4 in DAF which was constructed with computer software based on theory of homology modeling.

首次运用同源蛋白质结构预测的理论和*国家自然科学基金项目(39500031)：全军"九五"医药卫生科研基金资助项目方法建立DAF中SCR3和SCR4的三维分子模型，并对DAF的亲水性、可及性、二级结构及B细胞表位进行预测。

The stimulated lymphocytes assumed the appearance of lymphoblast showing morphological variations and leading to mitotic division.

上述结果提示表达蛋白不仅含有恶性疟原虫抗原T-/B-细胞表位，而且还具有IL-2的生物学活性。

Two kinds of mimetic peptides of PfEMP-1 have been obtained, which can bind with ICAM-1 specifically.

获得2种PfEMP-1噬菌体构象表位模拟肽，两短肽能与ICAM-1分子特异性结合。

Results Same conservative sites and key catalytic sites existed among SjLDH and LDHs from other species. Similarity of SjLDH compared to CsLDH, TvLDH and HsLDH was 75%, 17%, 58%-60% respectively. Phylogenetic analysis demonstrated that the evolution relation between SjLDH and DmLDH was closer than the relation between SjLDH and CeLDH, the relationship between SjLDH and HsLDH-B,-C was closer than HsLDH-A. Three trans-membrane regions were found, the region of 98aa-106aa in three hydrophilia regions located outside of membrane was inferred as the major antigen epitope. This antigen epitope had significant difference with LDHs from protozoon （ Pf ., Tg ., Tv .） and had 1-3 amino acid residue difference with MmLDH, HsLDH-A,-B,-C, and was the same with CsLDH. One of the key catalytic residues and substrate binding loop were located in this region. Tertiary structure demonstrated that 98aa-106aa was on the surface of the protein and formed a substrate binding loop, other two key catalytic sites were at the position near the loop.

结果 SjLDH与其他物种的同源序列含相同的保守位点及催化活性位点，与华支睾吸虫LDH同源性最高为75％，与阴道毛滴虫LDH同源性最低为17%，与人LDH（HsLDH-A，-B，-C）的同源性为58%~60％； SjLDH与果蝇的进化距离较秀丽隐杆线虫为近，3种人LDH中与HsLDH-B、-C的进化距离较HsLDH-A为近；该蛋白具有3个跨膜区域，3个高亲水性区域，主要的线性表位98aa~106aa位于膜外，与原虫LDH相同区域差异显著，而与其他LDH有1~3个氨基酸的差异，关键催化位点之一及底物丙酮酸结合区域位于该区域，蛋白质同源模建分析表明该区域位于蛋白表面形成环状结构，3个关键催化位点位于该区域或在其附近。

The E1 envelope protein is one of the structural protein of HCV,having important receptor binding site and epitope,it play important role in the process of viral infection and immunity.

HCV-E1包膜糖蛋白是HCV的结构蛋白之一，具有重要的受体结合位点和抗原表位，在病毒感染和宿主免疫过程中起着十分重要的作用。

Hepatitis C,caused by hepatitis C virus,is an important infectious disease threatening human health,and at present,there are no vaccines available for preventing HCV infection.The E1 envelope protein is one of the structural protein of HCV,having important receptor binding site and epitope,it play important role in the process of viral infection and immunity.

丙型肝炎是由丙型肝炎病毒(Hepatitis C virus， HCV )感染而引起的严重威胁人类健康的传染病，目还没有研制成功有效的疫苗 HCV-E1包膜糖蛋白是HCV的结构蛋白之，具有重要的受体结合位点和抗原表位，在病毒感染和宿主免疫过程中起着十分重要的作用。

Dot blot analysis of multiple human tissues showed that GSDML was restrictedly expressed in salivary.

针对GSDML蛋白序列的抗原表位，我们制备了GSDML的兔多抗和小鼠单抗，利用这些抗体检测到多种人源肿瘤细胞系均有GSDML内源性表达，而在鼠来源的NIH3T3和猴来源的COS-7细胞中未检测到其表达。

Results:(1) The HLA typing wasn't same completely in two chronic HCV patients and one self-limited patient.

2将这一表位与在GenBank已发表的34个HCV全长序列进行比较。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。