表位

(1) MGr1-Ag has an apparent molecular weight of 42KDa and an isoelectric point of 4.8. MGr1 epitope is composed of amino acids but not saccharide.

(1)MGr1-Ag是一种表观分子量约为42KDa、等电点约为4.8、抗原表位由氨基酸构成的蛋白质。

G The forecasting system on spatial coverage of cumulative phenotypic frequency of HLA class 1 for designing HLA-based vaccines in China was build by combing the Kriging model with the molecule immunology of HLA locus.

将"Kriging"模型与HLA基因座的分子免疫学相结合，建立了中国多表位疫苗设计的HLA－Ⅰ积累表型频率空间预测系统。

Property of antigens is reflected by code of antigen's epitope.

抗体自身的表位和对位反映抗体的特性，抗体可以构成免疫网络。

Containing many T cell epi-positions and neutralizing antibody epi-positions, the core protein region and envelope protein 2 of Hepatitis C virus structural protein may induce protective immunity responsion in body.

丙型肝炎病毒(hepatitis C virus，HCV)结构蛋白的核心蛋白和包膜蛋白2(E2)含有多个T细胞表位和中和抗体表位，可诱导机体产生保护性免疫应答。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Facing the present situations and the problems of verification for single-phase Watt-hour meter s in the electric power system,a new syncretizing design of the intel-lective and comprehensive testing equipment for great quantities of the meters is put forward in this paper.

针对电力系统中供电企业民用单相电能表检定工作的现状与存在的实际问题，提出了一种新的多表位智能化综合检测装置的一体化设计方案，详细介绍了基于该方案设计开发的测试装置的具体功能特点与性能指标，并对其实际应用情况进行了阐述。

To detect recombinant MUC1 with histag by flat-bed isoelectric focusing and provide reference for purification of recombinant MUC1 without histag by ion exchange chromatography.

目的采用薄层等电聚焦方法检测含有组氨酸尾的粘蛋白表位疫苗(简称重组MUC1)，为不含有组氨酸尾的粘蛋白表位疫苗的下一步离子交换层析纯化蛋白提供参考依据。

To detect recombinant MUC1 with histag by flat-bed isoelectric focusing and provide reference for purification of recombinant MUC1 without histag by ion exchange chromatography.

目的 采用薄层等电聚焦方法检测含有组氨酸尾的粘蛋白表位疫苗（简称重组MUC1），为不含有组氨酸尾的粘蛋白表位疫苗的下一步离子交换层析纯化蛋白提供参考依据。

P爱表族---全国领先的钟表论坛钟表名表论坛手表论坛媒体互动腕表主题社区 The origin of the Xa Flottiglia MAS can be traced back to the last days of World War I when two young Italian officers, Raffaele Rossetti and Raffaele Paolucci, sank the Austrian battleship (dreadnought-class) Viribus Unitis in the port of Pula.

|/ W/ W， b o h 在原产地的马航和XA Flottiglia可以追溯到的最后几天第一次世界大战时，两位年轻的意大利军官，拉菲尔尔罗塞蒂和拉斐尔保卢奇，奥地利战舰沉没 Viribus Unitis在港口普拉。

Specified determinant of hepatitis B surface antigen［J］.Acta Pathol Microbiol Scan,1975,83B:295?

V3、轮状病毒Vp4抗原表位和HCV抗原表位，并诱导中和抗体和交叉中和体产生［1，10，11］。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。