表位

The ideal epitope vaccine includes a group of optimized CTL epitopes, Th epitopes and B-cell epitopes.

理想的表位疫苗应包含一组优化的CTL表位、Th表位和B细胞表位。

Methods The recombinant sequence for two CEA epitopes was tandemly engaged and inserted into-upstream of in-frame-varied HSP70 of Mycobacterium tuberculosis to construct a gene vaccine.Balb/c mice were muscularly injected with recombinant DNA vaccine;negative control (mice were injected with normal saline), positive control (mice were injected with DNA vaccine harboring tandem CEA epitopes plus aluminium hydroxide adjuvant) and experimental group(mice were injected with PCITri CEA625-667-met HSP70) were set up.

在已经构建含有变异热休克蛋白序列的基础上，插入重组CEA串联表位的编码片段获得CEA串联表位-HSP融合基因疫苗。3次肌肉注射免疫Balb/c小鼠，设立注射生理盐水的阴性对照组、注射氢氧化铝佐剂混悬CEA串联表位的阳性对照组及注射pCITriCEA625-667-mtHSP70的实验组。

Methods Amplify the cDNA sequence encoding truncated HCV gene, with a part of carboxyl-terminus deleted, by PCR. Synthesize the mimic epitope at E2 region of HCV and seven T or Th cell epitope genes of NS3-NS5 respectively. Bind HCV core gene with a part of carboxyl-terminus deleted to the synthetic epitope gene by PCR, then clone into eukaryotic expression vector pcDNA3.1 and transiently transfect COS7 cells.

用PCR方法扩增核心区羧基端部分缺失的基因片段；分别合成HCV E2区模拟表位和NS3~NS5 7个T或Th细胞表位基因；PCR方法将羧基端部分缺失的HCV核心区基因与合成的表位基因串联，克隆入真核表达载体pcDNA3.1，并通过脂质体瞬时转染COS7细胞。

We hypothesize that the following mechanisms may be contributable to the help role of NV epitope for weak tumor antigen.

上述结果提示，NV抗原表位具有很强的辅助效应，它能明显增强机体对gp100CTL表位的免疫应答，进一步提高了以gp100为靶抗原疫苗的体内抗瘤效应，使得gp100弱势抗原表位得以优势化。

The 3D structure of N protein shows that the domain of Ep703 is exposed on the surface of the protein, making the epitope to be readily recognized by antibody. No homology is found between the mimotopes and native epitope. However, they all contain high content of hydrophile amino acids and low content of hydrophobile amino acids.

蛋白的三维结构显示，Ep703的9个氨基酸多肽暴露于蛋白的表面。3个模拟表位与表位Ep703的序列&IQTAFNQGA&相比，相同的氨基酸很少，但是各表位氨基酸的极性却有一个共同的特征：亲水性氨基酸比例较大，疏水性氨基酸比例较少。

Two of the six clones had the same DNA sequence,so there are five different antigenic epitopes in the six clones;the Blast analysis showed that there were no sequence homology between the five epitopes and the known antigen of paragonimus.

上述6个克隆有2个克隆的DNA序列完全相同，即6个克隆包含了5种不同的抗原表位；Blast分析表明5个表位与已知的肺吸虫抗原表位无DNA序列的同源性。

Meanwhile, positive human sera of HCV challenged vaccina ted monkeys after 6 weeks immunized, ALT value rose transiently in monkeys vacci nated by PCX. It was positive in monitoring RNA of HCV using RT-PCR in monkey s era. The result demonstrated that Rhesus monkey immunized by multi-epitope PCX could elicit high-level immune responses.

近年来，由于多表位抗原概念的兴起，在针对EB，HIV等病毒感染的疫苗研究中引入了多表位抗原疫苗［门，这种思路使对HCV疫苗的研究有了新的切入点，F.r.i［'］等用 HCV.H77株 E蛋白的多个表位所制备的兔血清与HCV

In this study, the DNA fragments coding for amino acids 133～158 of VP1and 20～34 of VP4protein of type Asial FMDV were chemically synthesized and ligated into a tandem repeat 133～158-20～34-133～158. The sequences of signal peptide of Igκ chain and Kozak were fused to the 5'end of this tandem sequence and synthetic oligodeoxynucleotide containing CpG-ISS was fused to downstream of terminal coden of this tandem sequence. And then this long fragment was cloned into the eukaryotic expression plasmid pHook-2, forming a new secreted expression plasmid, named pAS1-E.

在第二章证明了亚洲Ⅰ型口蹄疫病毒VP1蛋白中133～158位氨基酸残基确是一重要B细胞中和抗原表位的基础上，依据第一章获得的口蹄疫病毒亚洲Ⅰ型VP1 cDNA序列及已报道Asial VP4序列，采用真核偏爱密码子化学合成了VP1中编码133～158位氨基酸及VP4中编码20～34位氨基酸这两个抗原表位基因，将其组成133～158-20～34-133～158串联结构，在其5′端加上鼠Igκ链信号肽序列，在翻译调节区加上增强表达的Kozak序列，同时在133～158-20～34-133～158串联结构3'端终止密码子下游加上CpG免疫刺激序列，将这些片段连接后，克隆到真核表达载体pHook-2上，构建成功了DNA疫苗重组表达载体pAS1-E。

Methods Th multi-epitope peptide was made up of 5 Th epitopes(heat shock protein 65 of Mycobacterium tuberculosis p1-20,E2 protein of Rubella virus p54-65,engineering epitope PADRE,heat shock protein 60 of Chlamydia trachomatis p35-48,and tetanus toxin p830-843),and its gene sequence including of 219bp.

方法复合T辅助细胞表位由5个T辅助细胞表位组成(结核杆菌热休克蛋白65p120、风疹蛋白E2-4p5465、人工合成T辅助细胞表位PADRE、沙眼衣原体热休克蛋白60p3548、破伤风类毒素p830843)，其基因序列由219个核苷酸组成。

A multi-epitope antigen gene pcx was assembled by five highly conserved B-and/or T-cell epitopes, which were selected from C (1-16aa, 132-141aa), E1 (126-135aa), NS3 (139-147aa) and NS5B (768-775aa) protein, as well as a T-cell epitope of tetanus toxin, which was added to enhance T-cell immunity.

针对HCV的高度变异性及免疫特点，在HCV基因产物中优选了5个具有良好免疫原性的高度保守B／T细胞表位，组合成一个多表位抗原基因pcx，这些表位分别来自核心蛋白(1～16aa，132～141aa)，E1(126～135aa)，NS3(139～147aa)及NS5B(768～775aa)，以及破伤风毒素的T细胞表位用以增强T细胞免疫。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。