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And he caused his children to pass through fire in the valley of the son of Hinnom, and practiced soothsaying and enchantments and sorcery, and appointed mediums and spiritists; he did what was evil in the sight of Jehovah beyond measure, provoking Him to anger.

33:6 并在欣嫩子谷使他的儿女经火,又观兆,行法术,用邪术,立交鬼的和行巫术的,多行耶和华眼中看为恶的事,惹动祂的怒气。

Methods Male infertility couples from October 2006 to July 2008 were chosen and divided into ICSI group and TESA+ICSI group. ICSI group were 584 oligozoospermia cases with alive normal sperm in spermatic fluid. TESA+ICSI group were 54 obstructive azoospermia cases with alive normal sperm in testicle. Two groups were treated with ICSI after wives obtained oocytes by ovarian stimulation.

选择2006年10月~2008年7月因男性不孕症夫妇行ICSI治疗的患者638例分成两组,ICSI组和TESA+ICSI组,ICSI组为自行排精后精液内可以找到正常形态活精子的少精症患者行ICSI治疗584例,TESA+ICSI组为梗阻性无精子症患者经睾丸穿刺找到睾丸内形态正常活精子行ICSI治疗共54例,两组女方均给予超促排卵用药获取卵母细胞。

We used ITO glass substrate and high purity(99.99%) titanium , put to use sputtering process, add to high purity O2 make it complete the best structure film and the thickness about 1400 . By way of 500℃ anneal could got a better anatase phase, this result could easy reaction with electrolyte to increase electron-transferred. Used as spin casting covering PR on ITO substrate, transferring patterns of array circular shapes on a mask to photography, used optimize temperature and time to hard bake. Finally used HCl to etching underexposed TiO2 area, and acetone to tripper top PR, make it to accomplish pillars shape TiO2 electrode.

本研究使用ITO玻璃基板为底材在以高纯度钛(99.99%)为靶材,利用溅镀方式通入纯氧使其行成高纯度TiO2镀於ITO基板上行成厚度约1400的薄膜,且经500℃高温烧结使其行成最强的锐钛矿结晶相,此晶相有利於与电解液反应进而增加电子的转移,提升太阳能电池最佳的效率,在以旋转涂布方式铺上光阻,以矩阵排列的圆孔光罩曝光、最佳的温度及时间来烘烤、显影后;使用盐酸以等向性湿蚀刻方式蚀刻未曝光之TiO2区域,最后利用鈵铜将最上层光阻去除,使其行成柱状TiO2的电极。

The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.

1、健康雌性SD大鼠18只,随机分为电穿孔组和非电穿孔组,每组9只,制作右下肢趾长伸肌失神经支配模型;EP组为将质粒pEGFP-N1溶液50μl(0.8μg/μl)注射入右趾长伸肌后,立即于两侧腱腹交接处给予电穿孔,电穿孔参数为:电场强度为200V/Cm,脉冲100μs,频率1Hz,施加10次脉冲;NEP组仅质粒pEGFP-N1溶液注射;转染后1、2、3周,荧光显微镜下观察趾长伸肌中GFP的表达情况,转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况,检测和优化体内转染效率。2、健康雌性SD大鼠78只,随机分为失神经对照组、RC2基因治疗组(RC2组),MAFbx基因治疗组,每组24只,制作右下肢趾长伸肌失神经支配模型,余6只为正常组;分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌,之后给予电穿孔,方法同上;治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化,治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积,肌细胞超微结构变化以及肌纤维中蛋白含量变化。

Middle: from Kazakhstan to Russia, Ukraine, Slovakia, Hungary, Austria, Switzerland, Germany, France to the English Channel port to the sea or from Kazakhstan Aktau south, along the border of Kyrgyzstan and Turkmenistan via Uzbekistan, Tashkent Ashihe Bardsea trip to Krasnovodsk, too, Getafe Azerbaijan Baku, via Georgia Tbilisi-quarter of Hong Kong and Vietnam to Bulgaria's Varna Black Sea and the northern enter Romania, Hungary to the EU countries.

中线:由哈萨克斯坦往俄罗斯、乌克兰、斯洛伐克、匈牙利、奥地利、瑞士、德国、法国至英吉利海峡港口转海运或由哈萨克斯坦阿克套南下,沿吉尔吉斯斯坦边境经乌兹别克斯坦塔什乾及土库曼斯坦阿什哈巴德西行至克拉斯诺沃茨克,过里海达阿塞拜疆的巴库,再经格鲁吉亚第比利斯及波季港,越黑海至保加利亚的瓦尔纳,并经鲁塞进入罗马尼亚、匈牙利通往中欧诸国。

Ten patients with temporal bone fracture and trauma of ear,whose CT scan of temporal bone showed that Malleoincudal joint irregular shape in the axial view and incudostapedial joint dislocation in the coronal view,were included in this study,Virtual endoscopy showed Malleoincudal joint separation,incudostapedial joint dislocation and interruption,ossicular chain disruptio...

虚拟耳镜示锤砧关节分离,砧镫关节脱位,听骨链中断。手术经颞线下开放上鼓室,保留完整的外耳道后上壁,充分暴露上、后鼓室和听骨链,检查并行听骨链成形术。结果:砧骨长脚骨折1耳,将砧骨改型后行锤-镫连接术;锤砧和砧镫关节脱位6耳,砧骨完全脱落3耳,行砧骨复位双关节固定术。10耳均行上鼓室进路鼓室探查术,术后语频听力平均提高25~50dB。结论:完壁式上鼓室进路鼓室探查术治疗外伤性听骨链中断疗效满意。

Methods: 37 patients with complex upper urinary lithangiuria with pyonephrosis were selected during November 2002 to July 2007. 8 cases were treated with transurethral ureteroscope lithotripsy followed by tube internal drainage, while 29 cases were treated with minimally invasive percutaneous nephrolithotomy. After 714 days, minimally invasive percutaneous nephrolithotomy was employed for the second therapy.

选择2002年11月~2007年7月复杂上尿路结石合并脓肾患者37例,采用经尿道输尿管镜取石后置管内引流8例,微创经皮肾造瘘外引流29例,术后7~14 d后,2期行微创经皮肾镜治疗复杂肾结石。

Methods The surgical methods to resect pathologically confirmed parotid mixed tumor in 60 cases from January 1998 to December 2002 was analyzed retrospectively. Resection of tumor combined with partial parotidectomy was applied to 58 cases with maximal tumor diameter less than 4cm and total parotidectomy for 2 cases with maximal tumor diameter greater than 4cm.

对我院及上海第二军医大学附属长征医院1998年1月~2002年12月收治的60例经病理诊断的腮腺混合瘤患者行手术切除的方式进行分析。58例肿瘤最大直径小于4cm、患者行肿瘤及周围部分腮腺切除术;对2例最大直径超过4cm的肿瘤患者行腮腺全切除术。

objective to evaluate the main points and complications of ct-guided percutaneous pneumocentesis in patients with lung lesions.methods cook 18~21g puncture needle was used to inserting aspiration in the optimal level,situs,angle and depth.and had made of multidrop.fifty-nine patients with lung lesions underwent ct-guided percutaneous pneumocentesis and follow-up.results the successful puncture rate was 100%,accurate diagnose rate was 92%(54/59).conclusion ct-guided pneumocentesis for chest lesion is a efficient and safe diagnostic method.but it is important to improve the doctor′s operating skill to lessen vascular complication.

目的 探讨ct引导下经皮肺穿刺活检的技术要点及并发症。方法采用cook公司18~21g自动弹射活检枪,选择最佳层面、进针点、进针角度和深度,行多点取材。回顾性分析59例肺内病变ct引导下经皮肺穿刺活检术。结果穿刺成功率100%,活检诊断准确率为92%(54/59)。3例发生轻度气胸,2例穿刺后咯血痰。结论 ct引导下经皮肺穿刺活检术成功率高,是安全的,但操作医生熟练的技术方法非常重要,并可以减少并发症的发生。

Methods 38 cases with 53 hips of ANFH treated with the method of thrombolysis and opening catheterizing and perfusing medial and lateral circumflex arteries.Those drugs are Urokinase 200 thousand Units,Danshen Root 20 ml and Ligustrazine 20 mg. Retention catheter was held in the target vessels and infused the same drugs besides Heparin 4500u in 3 days. To evaluate therapeutic effect ,the DSA of the femoral artery and x-ray plain film and/or CT/MRI of the femoral head were performanced. Results All cases were followed up 1~16 (mean 9.5) months.

方法对38例ANFH患者的53髋先常规行股动脉DSA,后在股骨头的供血动脉旋股内、外侧动脉内灌注溶栓、扩血管药物,即在每支血管内注入稀释后的尿激酶 20万u、丹参10 ml及川芎嗪20 mg;术后保留导管于动脉内,经导管每日注入同剂量的药物及肝素钠4500 u,连用3天后拔管,治疗后复行股动脉DSA检查及行股骨头X线平片及/或CT、MR随访以评估疗效。

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