血量减少

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

RESULTS: After ischemia-reperfusion injury, the rats had hydrouria, urine osmotic pressure depress, symptoms of carnine and urea nitrogen increasing. HE staining demonstrated that renal tubular epithelial cells were swelling, necrosis, and desquamate. Aquaporin-1 expression and its mRNA level was decreased; in particular, the expression and level were the lowest at day 1 after ischemia-reperfusion injury and recovered to normal value at day 5 after ischemia-reperfusion injury.

结果：大鼠肾脏缺血再灌注损伤后出现尿量增多、尿渗透压降低，血肌苷、尿素氮升高症状；苏木精-伊红染色示肾小管上皮细胞肿胀、坏死、脱落；水通道蛋白表达量及其mRNA含量均较对照组减少，这些变化于缺血再灌注损伤 1 d时最低，至缺血再灌注损伤 5 d时恢复正常。

The patient showed atrophy in frontal and temporal lobe on regular MRI, significantly reduced amount of Nacetyl aspartic acid、cholineand creatinecompared with contralateral on MRS , remarkably decreased blood flow in the left temporal polar and front part of fontal lobe compared with contralateral on PWI. There were decreased association fibers between Broca and other regions meanwhile no association fibers were found between triangular area and wernicke region on DTI. The fibers projecting to opercular part of frontal lobe via archiform fibers were lessened. The mean FA and fibers of Broca and Wernick regions were less than that of the healthy subjects.

常规MRI显示左侧额叶及颞叶萎缩；磁共振波谱分析显示左侧颞叶和额叶N乙酰天门冬氨酸、胆碱、肌酸含量较对侧明显减低；磁共振灌注成像显示左侧颞极及额前部的血流量较对侧减低；弥散张量成像显示左Broca区与其他脑区间纤维联系减少，三角区与Wernicke区无纤维联络，左侧Wernicke区通过弓状纤维到达额叶岛盖部的纤维减少，Broca及Wernicke区平均FA及纤维束较正常人减少。

The effects of QDPTP on cerebral blood flow and artery blood pressure in anesthetized rats The regional cerebral blood flow in cerebral pia mater was examined by Laser Doppler Flowmeter and the artery blood pressure (including mean systolic pressure, mean diastolic pressure and mean blood pressure) were monitored by the electrophysiological polygraph before and after drug administration.

结果表明：模型组脑组织及血清IL1-β含量分别为1006.4±247.1 pg/ml和370.8±76.6 pg/ml，与之相比，QDPTP中剂量(550.7±99.8，268±64.6)组中脑组织及血清中IL1-β的含量显著降低(P.01)，川芎嗪脑也明显减少组织IL1-β的含量(627.8±144.7，P.05)。 QDPTP对麻醉大鼠局部脑血流量和动脉血压的影响采用激光多普勒血流仪测量大鼠软脑膜局部的血灌注量，其被视为局部的脑血流量。

Thrombocytopenic purpura is characterized by its hazard a significant reduction in platelets, associated with purpuric skin and mucous membrane, severe bleeding may have other parts such as nose bleeding, gum bleeding, women's menstrual excessive or severe hematemesis, hemoptysis, hematochezia, hematuria, and other symptoms , concurrent intracranial hemorrhage is the cause of death in this disease.

血小板减少性紫癜其危害的特点是血小板显著减少，伴有皮肤粘膜紫癜，严重者可有其它部位出血如鼻出血、牙龈渗血、妇女月经量过多或严重吐血、咯血、便血、尿血等症状，并发颅内出血是本病的致死病因。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

Reduce presystolic and systolic mitral and tricuspid regurgitation resulting to increase time available for forward flow.

2在收缩期及收缩期前减少二尖瓣返流，增加心室前向排血量。

Fter different detraining cycles on the physiological performance, the detraining cycles will be reflected different degrees of impact. We found that the strength and endurance of athletes in detraining has reduced the capillary density, oxidative fibers, slow-twitch muscle. On the other hand, AVO2-diff, oxygen enzyme decline in the number of mitochondria, the change of muscle fiber types and reduced neuromuscular control in long-term detraining. In cardio-respiratory fitness, particularly in endurance athletes among blood flow, plasma volume, and cardiac output's decline are the most conspicuous. After hormone and metabolic's short term and long-term detraining, we found that growth hormone, testosterone, cortisol, respiratory exchange rate, showed a decline in insulin sensitivity, it will directly affect the exercises performance. Further more, the exercises performance and physiological benefits could have been completely eliminated.

bstract 不同周期停止训练后，对生理表现将反映出不同程度之影响，其中发现肌力性与耐力性运动员在短期停止训练后，毛细血管密度、氧化纤维、慢缩肌呈现减少情形；反观长期停止训练后，将会使动静脉血含氧差异(arterial-venous oxygen difference， AVO2-diff)、氧化酶粒腺体数目下降与肌纤维型态之改变，而减少神经肌肉控制能力、在心肺适能方面，尤以耐力性运动员在血流量、血浆量、每跳输出量、最大心输出量下降情形最明显；而贺尔蒙与代谢在短期与长期停止训练后，发现生长贺尔蒙(growth hormone， GH)、睪固酮、可体松、呼吸交换率、胰岛素敏感性均呈现下降情形，这些情形将直接影响其运动表现之发挥，甚至使先前获得之运动表现与生理效益完全消失。

The intake of fats,protein was higher than drls,while the carbohydrate vitamin a,vitamin b2 and some minerals was not sufficient to meet the demand of body.of all the 836 officers,there were many more people whose body mass was excessed the standard.and there were some people who had hypertension,hyperglycemia,hyperlipemia,hyperuricemia and fatty liver.conclusion the total energy of food can meet the need of the officers,but the food structure is not reasonable.therefore,it is necessary to adjust the food structure,increase the intake of grains,beans,milk,fruits and vegetables,decrease the intake of fat,salt and alcohol and diversify the food items.

食物摄入总热量基本符合要求，但分布不够合理。脂肪和蛋白质的摄入量偏高，碳水化合物摄入过低；维生素a、维生素b2及钾、钙、镁的摄取量偏低；836名高职在职干部体质量超标较为严重，部分患有高血压、高血糖、脂肪肝、高脂血症及高尿酸血症。结论食物摄入总能量基本符合要求，但饮食结构欠合理。建议调整饮食结构，适当增加谷类、豆奶类食物及果蔬类食物摄取，减少脂肪、食盐及酒精的摄入，提倡食物品种多样化。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐