血过氧化氢酶

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

To apply 5% H2O2 on the black skin of guinea pigs,2 daily,for 50 days,and draw blood from abdominal aorta,and determine the content of tyrosinase by spectrophotometer and indexes of hemorrheology by LIANG-100 blood rate viscosimeter.

用5%过氧化氢涂布于豚鼠的黑毛区，每日2次，连续50d，腹主动脉取血，用分光光度计测酪氨酸酶含量和用LIANG-100型血液比粘度计测血液流变性指标。

Carnosine was added to the drinking and addition dose atⅠto Ⅳgroups were 0,20,40 and 60 mg/L respectively for 6 weeks.6 broilers from each group were randomly selected to pick the blood respectively at the each weekend.

于每周末每组随机抽取6只鸡采血，测定血清中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶的活力和丙二醛的含量。

The weight was measured and the food intake was recorded every week. Fasting blood was collected to check total cholesterol in serum once every two weeks. The experiment was end in 12 weeks. Under anesthesia,the blood was collected from hypogastric aorta to check the level of serum blood lipid and the activities of superoxide dismutase and catalase.

每周称重，记进食量，每隔两周采空腹血测定血清总胆固醇，12周后实验结束，麻醉下腹主动脉采血检测血清血脂水平和超氧化物歧化酶、过氧化氢酶的活性。

Litopenaeus vannamei were continuously fed by basic diets containing three levels (1%, 0.5%, 0.1%) Ig-Guard respectively for 20 days, and the basic diets as blank control. Phenoloxidase, acid phosphatase, alkaline phosphatase, lysozyme activity of the serum and superoxide dismutase, catalase activity of the muscle, and protein concentration of the serum and the muscle were detected in the 5, 10, 15, 20 day.

采用连续投喂的方法，饲喂凡纳滨对虾 20 d，分别测定了第5，10，15，20 d血淋巴的酚氧化酶、溶菌酶、酸性磷酸酶、碱性磷酸酶及肌肉匀浆液的超氧化物歧化酶、过氧化氢酶等非特异性免疫因子活性，并对血清及肌肉匀浆液中蛋白进行定量。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。