血蓝蛋白

Methods: Three polypeptides were synthesized based on the bioinformatics analysis of the CMTM7 and coupled with keyhole limpet hemocyanin. Balb/c mice were immunized with these mixed CMTM7 polypeptides.

通过生物信息学对CMTM7的蛋白序列进行分析，选取了3段序列合成多肽并与钥孔戚血蓝素(keyhole limpet hemocyanin， KLH)偶联，混合后免疫Balb/c小鼠。

Antioxidant activity of Se-PC was investigated and compared with phycocyanin by using four different free radical scavenging assays, namely, trolox equivalent antioxidant capacity assay, DPPH free radical scavenging assay, superoxide anion scavenging assay and assay for erythrocyte hemolysis mediated by peroxyl free radicals .

通过含硒藻蓝蛋白与藻蓝蛋白对4种不同的自由基清除能力的比较，即总抗氧化能力、清除DPPH自由基、过氧化物阴离子和引发红细胞溶血的过氧化氢自由基能力测定，从而对含硒藻蓝蛋白的抗氧化活性进行评价。

Methods:The peptide sequences of human CYP1A2 were analyzed according to its bioinformatics, and synthesized on the basis of hydrophilicity, antigenicity, accessibility and flexsibility. The synthesized peptides were crosslinked with keyhole limpet hemocyanin, which was used to immunize rabbits for production of Ab.

利用生物信息学方法分析CYP1A2蛋白的序列，根据亲水性、抗原性、柔韧性及表面性等指标选择多肽序列，合成CYP1A2多肽，与载体蛋白钥孔戚血蓝素（Keyhole limpet hemocyanin， KLH）偶联，免疫日本大耳白兔制备抗CYP1A2抗体。

METHODS: Two polypeptides named peptide 1 and 2 were synthesized based on the bioinformatics analysis of the sequence of hMR-1 by using software TMHMM and DNAStar, then coupled with keyhole limpet hemocyanin for immunization.

TMHMM、DNAstar等软件分析hMR-1蛋白序列，选取2段优势抗原序列合成多肽并与钥孔戚血蓝素偶联，混合后免疫家兔。

Methods ①Human peptides of CYP450 enzymes and their proteinic sequence were analyzed by means of bioinformatics, and certain sequences were chosen and synthesized according to four indexes (Hydrophilicity Plot, Flexible Regions, Antigenic Index and Surface Probability Plot) after homology analysis by software Blast. The synthesized peptides were conjugated to keyhole limpet hemocyanin to increase antigenicity.

①利用生物信息学方法分析CYP1A2、CYP3A4及CYP4A11蛋白的序列，利用DNAstar软件中蛋白质的亲水性结构、柔韧区、抗原指数及表面概率结构指标选择多肽序列，应用Blast软件对所选序列进行同源性分析并合成多肽，与载体蛋白钥孔戚血蓝素偶联，免疫大耳白兔制备抗CYP1A2、3A4及4A11抗体。

Methods Phosphotyrosine was first combined with hemocyanin from keyhole limpets. Then, rabbits were immuned with the complete antigen and antisera were gained. The ELISA system consisted of purified antiphosphotyrosine antibody as first antibody and sheep antirabbit lgG-HRP as second antibody. Results CV within the batch was 8.4%.

应用合成的磷酸酪氨酸-钥孔帽贝血蓝蛋白免疫家兔得到抗血清，将纯化的抗磷酸酪氨酸抗体作为第一抗体，羊抗兔Ig酶结合物作为第二抗体，建立双抗体夹心酶联免疫吸附测定法。

Mouse monoclonal antibodies against salicylic acid was produced and characterized for the first time. The McAb S2-8F, were derived from an immunogen in which 5-aminosalicylic acid (5-ASA) was conjugated to KLH (hemocyanin from keyhole limpets) through its -NH〓 group. The antibody showed high specificity to SA and even more high specificity to 5-ASA (the cross-reaction is 144. 8％), but very little cross-reactivity with 5-sulfosalicylic acid (0.9％) and 4-aminosalicylic acid (0.3％). Other compounds structurally similar to SA, such as 4-hydroxybenzoic acid, 4-aminobenzoic acid and salicylaldoxime, were not found to exhibit any cross-reactivity.

以5-氨基水杨酸(5-aminosalicylic acid，5-ASA)的5位氨基为偶联位点，血蓝蛋白(hemocyanin from keyhole limpets，KLH)为载体合成了SA-NH-CH〓-NH-KLH，以此为免疫原免疫小鼠，利用杂交瘤融合技术得到三株可产生针对SA抗体的细胞株，并对其中编号为S2-8F的细胞株产生的单克隆抗体的特性进行了研究，发现它对SA和5-ASA具有高亲和力。

Upon activation, there is an overall conformational change of the complex.

在活化的过程中，血蓝蛋白复合物会产生构象上的全方位的转变。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了