血蓝蛋白

OBJECTIVE:To observe the characteristics of Th1/Th2 imbalance in splenocytes derived from Balb/C mice immnnized by keyhole limpet hemocyanin.

目的：分析钥孔戚血蓝蛋白诱导Balb/C小鼠脾细胞Th1/Th2失衡的特点。

Methods PIWIL4 peptide was synthesized using chemical method, and conjugated to Keyhole limpet hemocyanin as immunogen.

方法合成特异性PIWIIA多肽，以马来酰胺活化匙孔血蓝蛋白作为载体构建多肽免疫原，通过致敏大白兔，制备兔抗人PIWIIA多克隆抗体，然后用亲和层析法纯化抗体。

When Dr. Yao Cong, a postdoctoral researcher in the laboratory of Dr. Wah Chiu, displays the computer representation of hemocyanin, it glows like a four-part jewel on the computer screen.

当与Wah Chiu 博士同在一个实验室的姚聪博士后，用计算机展示了血蓝蛋白的图像时，它简直就像由四部分组成的宝石在计算机屏幕上闪烁不定。

Specific polyclonal antibodies against SBP117 are successfully raised by synthesized peptides which were conjugated with Keyhole Limpet Hemocyanin and used as antigens to immunize rabbits.

人工合成SBP117的两个肽段与匙孔嘁血蓝蛋白载体偶联后免疫兔子，获得了抗SBP117的专一性抗体。

In the paper, a preliminary study was made to analyze the interaction between hemocyanin, which was purified from shrimp P. vannamei obtained from Shantou, and anti -human antibody. The methods of Sephadex G-75 column chromatography, PAGE, Dot-ELISA, SDS-PAGE and Western-blotting were used.

以采自汕头的南美白对虾血清为研究对象，运用Sephdex-75柱层析、PAGE、Dot-ELISA、SDS-PAGE和Western-blotting等方法，探索血蓝蛋白与抗人IgG之间的相互作用。

Mice immunized with MAD coupled with keyhole limpet hemocyanin could produce specific antibodies against MAD, and the antibodies obtained could bind not only native HCMV particles, but also the recombinant gM30～78 peptide.

将MAD与钥孔血蓝蛋白偶联免疫小鼠可产生抗MAD多抗，该多抗不仅结合天然HCMV病毒粒子，而且特异结合重组表达的gM30～78多肽。

METHODS: Female BALB/c mice were treated with CCK-8 and keyhole limpet haemocyanin, splenocytes were acquired and incubated in vitro with KLH restimulation, the productions of Th1 cytokines, interferon-γ, interleukin-2 and Th2 cytokines, IL-4, IL-5 in cell culture supernatants were detected by enzyme-linked immunosorbnent assay. The mRNA expressions of IFN-γ, IL-2, IL-4 and IL-5 in splenocytes were detected by reverse transcription-polymerase chain reaction. The levels of Th1 antibody IgG2a and Th2 antibodies IgG1 in serum were also detected by ELISA.

给予BALB/c小鼠钥孔戚血蓝蛋白免疫同时体内给予不同剂量的CCK-8，酶联免疫吸附试验检测其脾细胞培养上清中Th1型细胞因子γ-干扰素、白细胞介素-2（IL-2）和Th2型细胞因子白细胞介素-4（IL-4）、白细胞介素-5（IL-5）水平，逆转录聚合酶链式反应法检测脾细胞中IFN-γ、IL-2、IL-4、IL-5 mRNA表达；ELISA法检测血清中Th1型抗KLH抗体IgG2a和Th2型抗KLH抗体IgG1水平。

Methods PIWIL polypeptide was synthesized and conjugated to Keyhole limpet hemocyanin as an immunogen. Then PIWIL-KLH conjugations were injected into rabbits subcutaneously to produce polyclonal antibodies.

合成特异性PIWIL多肽，以马来酰胺活化匙孔血蓝蛋白作为载体构建多肽免疫原，通过致敏大白兔，制备兔抗人PIWIL多克隆抗体，用亲和层析法纯化抗体。

Methods Fifteen healthy, female SD rats were employed in the experiment. Sensitized systematically with keyhole limpet hemocyanin, the animals were inoculated with the same antigen through cochlea basal turn into the labyrinth. Adminstrated 5-bromo-2′-deoxyuridine intraperitoneally, the rats were sacrificed and the temporal bones were harvested at 3, 7,14 day after labyrinth vaccination respectively. The frozen sections of the decalcified samples were dealt with H-E staining and immunohistohemical methods to investigate the cellular infiltration, BrdUrd and IgG positive cells in the ES.

选用SD大鼠15只，以钥孔虫戚血蓝蛋白（keyhole limpet hemocyanin，KLH）全身免疫后，经耳蜗底回钻孔以相同抗原进行内耳免疫，然后分别在内耳免疫后3、7和14 d腹腔注射溴脱氧尿嘧啶核苷（bromodeoxyuridine，BrdUrd）后处死动物，取颞骨经组织学处理制作冰冻切片，用免疫组织化学技术观察内淋巴囊的细胞浸润、增殖和IgG细胞的分布状况。

Methods AGOs polypeptide was synthesized by chemical method, and conjugated to Keyhole limpet hemocyanin as immunogen. Then AGO-KLH conjugations were injected into rabbits subcutaneously to produce polyclonal antibodies.

合成特异性AGO多肽，以马来酰胺活化匙孔血蓝蛋白(keyhole limpet hemocyanin， KLH)作为载体构建多肽免疫原，通过致敏大白兔，制备系列兔抗人AGO多克隆抗体，然后用亲和层析法纯化抗体。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了