血脑屏障

Hooper, previous studies by his group and other researchers have shown that blood-brain barrier permeability is critical in the development of MS.

为了研究血脑屏障的通透性，他和他同事们观察到多发性硬化进展过程中血脑屏障的损伤起到关键作用。

Methods: Mice were inoculated intracerebrally with herpes simplex virus type1(HSV-1) to form herpes simplex encephalitis. MMP-9 gelatinolytic activity was assessedby zymography from brain tissues 3 days and 7 days after HSV-1 infected.

第一部分单纯疱疹病毒性脑炎相关病理机制的研究实验一MMP-9在单纯疱疹病毒性脑炎中的表达及对血脑屏障的影响目的：研究单纯疱疹病毒感染小鼠脑组织后MMP-9的变化及对血脑屏障通透性的影响。

Neuroglia, the basic integrant of blood-brain-barrier is defective in brain metastatic tumor tissue, thus there is no blood-brain-barrier existing in brain metastatic tumor lesions.

脑转移癌组织缺乏构成血脑屏障的神经胶质膜，血管内皮也非均一的紧密连接，所以脑转移癌组织不存在完整的血脑屏障。

MethodsBorneol contained serum was prepared and using Matin-Darby canine kidney epithelium cell line as the in vitro BBB model to observe the effects of borneol on intercellular tight junction and pinocytosis vesicles of BBB model.

方法制备含冰片的家兔血清，应用马丁达比狗肾上皮细胞系作为血脑屏障的体外模型，观察冰片对血脑屏障细胞间紧密连接、细胞吞饮囊泡的影响。

We discovered that the mechanisms of borneol opening BBB involved (1) promoting the tight junction of endothelial cells opening:(2) increasing the quantity of pinocytosis vesicle in endothelial cells and enhance the pinocytosis;(3) inhibiting the activation of P-glycoprotein (P-glycoprotein is an efflux pump of drugs) and increase the permeability of BBB;(4) reducing the expression of intercellular adhesion molecule-1 in brain microvessel endothelial cells;(5) increasing the concentration of Ca〓 in brain microvessel endothelial cells;(6) increasing the activation of eNOS in brain microvessel endothelial cells.

随后，在对冰片开放血脑屏障的机制作进一步的研究时又发现，冰片开放血脑屏障的机制包括以下几个方面：(1)冰片可使血脑屏障内皮细胞间的紧密连接开放；(2)冰片能使内皮细胞内的囊泡数量增加，吞饮功能增强；(3)冰片能抑制P-糖蛋白的活性（P-糖蛋白是一种药物外排泵），而使血脑屏障的通透性增加；(4)冰片能使脑微血管内皮细胞ICAM-1表达量减少；(5)冰片使脑微血管内皮细胞内的Ca〓浓度升高；(6)冰片可升高脑微血管内皮细胞eNOS的活性。

Part 1: Permeability of Exogenetic BDNF through placental barrier of pregnant rats and blood-brain barrier of fetus with transient uteroplacental ischemiaObjective To investigate the potential of BDNF to penetrate the placental barrier and get into the fetus and pass through the blood-brain barrier of fetus to play neuroprotective function in the condition of transient ischemia.

第一部分：外源性脑源性神经营养因子在宫内缺氧环境中对胎盘及胚鼠血脑屏障通透性的实验研究目的：探讨外源性脑源性神经营养因子在宫内缺氧环境下能否通过胎盘屏障进入胎鼠体内，再通过胎鼠血脑屏障进入胎鼠脑组织而发挥其生物功能。

Methods Brain microvascular endothelial cells and neuroglial cells of newborn rats were cultured in vitro, and treated with 1,2-dichloroethane at a dose of 0 μl/ml, 5 μl/ml, 10 μl/ml or 20 μl/ml.

方法］在体外建立了血脑屏障细胞模型，并从形态学上观察1，2-二氯乙烷对血脑屏障细胞的损伤作用。

Interpretation NMO-IgG is a specific marker autoantibody of neuromyelitis optica and binds at or near the blood-brain barrier.

NMO-IgG抗体为视神经脊髓炎的特异性自身抗体标志物，在血脑屏障或血脑屏障附近结合。

They are already being evaluated in clinical trials as potential therapeutic agents for major human neurodegenerative diseases, such as Alzheimer's disease, Parkinson's disease and motor neuron disease. However, delivery of polypeptide growth factors to compromised neurons in the CNS is problematic because their plasmatic half-life are very short and their abilities to cross the blood-brain barrier are theoretically null.

然而由于中枢神经系统中存在血脑屏障，神经营养因子类物质难以通过血脑屏障而到达靶部位；同时又因为神经营养因子在体内生物半衰期较短，使得全身性用药效果不够理想，另外还往往产生意想不到的副作用，如何解决这些问题对于深入研究神经营养因子在神经损伤及神经系统退行性疾病中的作用及开展临床应用前景研究有着十分重要的意义。

Objective To study the dynamic changes of transendothelial electrical resistanceonourin vitromodelofbrain blood barriermade from primary culture ofBALB/cmouse brainmicrovascu-lar endothelial cells,and to explore the relation between TEER and BBB permeability,and searchfor the best culture condition.

目的 应用原代分离培养BALB/c小鼠脑微血管内皮细胞(brain microvascular endothelial cells， BMVEC)建立体外血脑屏障模型，探讨跨血脑屏障(blood brain barrier，BBB)电阻与屏障渗透功能的动态关系以及最佳构建条件。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。