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The studies on the capacities of immune responses of lymphocytes in peripheral blood and immune organs of Grass Carp (Ctenpharyngoden idellus Cuvier et Valencinnes) to PHA and SPA with incorporation of 〓H-TdR and active reaction of Acid α-Naphthyl Acetate Esterase indicated that there were T and B lymphocytes in peripheral blood of grass carp, but only T lymphocyte and no B lymphocyte in the thymus of grass carp. It was justified that the T lymphocytes in peripheral blood was originated from the thymus, and B lymphocyte could be from the spleen and anterior kidney of grass carp.

运用〓H-TdR掺入法和酸性醋酸萘酯酶活性检测法分别研究了草鱼外周血以及免疫器官中的淋巴细胞对PHA和SPA的免疫应答以及化学标志特征,发现草鱼外周血中存在着类似于哺乳动物的T、B两类不同的淋巴细胞亚群;同时也发现胸腺中只有T淋巴细胞,而无B淋巴细胞,提示了胸腺中淋巴细胞可能是外周血中T细胞的来源,而B淋巴细胞可能来自脾脏或前肾。

Methods The presence and localization of the dilatant nasal mucosa capillaries in proband was determined by endoscope of nasal cavity; clinical diagnosis of HHT was established by obtaining the patient's bleeding history, family history, and observation of bleeding sites.

完善血常规、肝肾功能和胸片、腹部B超、血氧饱和度等检查;进行出凝血时间、血块回缩率、血小板聚集功能等检查;对与先证者有血缘关系的家系成员进行胸片、腹部B超、血氧饱和度检查,以排除和诊断HHT,绘制HHT遗传系谱图。

Methods The contents of many kinds of elements such as Ca, Zn, Cu, Fe, Se, Pb, Cd, Hg were respectively detected in cerebra, gluteu, thymus, occipital bone, liverof congenital malformed and inevitable abortion foetus at the age of five to eight months and correlative gravida's blood, umbilic blood, placenta, and the contents of vitamin A from gravida's blood and umbilic blood.

分析5~8个月龄先天性畸形胎儿和难免流产胎儿大脑、臀肌、胸腺、枕骨、肝脏组织及与其相关的母亲血、脐血、胎盘中钙、锌、铜、铁、硒、铅、镉、汞以及其母亲血、脐血中维生素A的含量,并与正常对照组比较。

Objective To compare the changes of the number of the blood cell and coagulatory functions in the intracavitary blood before the use of Autologous Blood Recovery System and after.Methods The RBC,Hct,Plt,PT,APTT,FIB,TT of the intracavitary blood were measured in thirty-two patients with acute closing intraabdominal and intrathoracic hemorrhage.

目的 比较自体血液回收机处理后胸/腹腔内积血血细胞量和凝血功能变化的特点方法 32例急性闭合性胸/腹腔内出血病人,分别检测自体血液回收机处理前后体腔内积血的红细胞数、红细胞压积、血小板、凝血酶原时间、部分凝血酶原时间、纤维蛋白原、凝血酶时间。

Results 305 patient underwent VATS procedures. The overall incidence of perioperative complications (hemopneumothorax: 3 cases) was 0.98%(3/305), and no patient died during the operation.

结果 305例患者均痊愈出院,围手术期死亡率为0,并发症(血胸3例)发生率为0.98%(3/305)。

Methods 150 patients with pulmonary contusion were analyzed. The CT image and the clinical data was collected, and analyzed the correlativity between delayed pulmonary contusion and the occurred time, the position, previously lung disease or the delayed hemopneumothorax.

分析150例迟发性肺挫伤的CT和临床资料,对迟发性肺挫伤与发生时间、发生部位、与既往肺部疾病以及迟发性血胸的相关性进行研究,对迟发性肺挫伤的CT表现及其动态变化等进行分析。

Methods Twelve dogs were randomly divided into experimental and control groups (n=6). They were treated with two kinds of thoracic cavity closed drainage system respectively after artificial hemopneumothorax. The time of thoracic cavity closed drainage, anti-tensile strength,and hemoglobin contents in peripheral venous blood before operation and after reinfusion were observed.

对比分析自行研制的新型多功能胸腔闭式引流装置与现有的胸腔闭式引流装置对实验犬(n=6)及对照犬(n=6)的多项指标的影响,如胸腔闭式引流术所用时间、两种装置的抗拉力、引流胸血及胸血回输情况和创伤前及回输后周围静脉血液血红蛋白含量的变化以及动物生存情况等。

RESULTSThe branches of aortic arch are the left and right innominate artery in pate of mini-pig.

结果小型猪主动脉弓部向头颈部分支为左、右无名动脉;双侧颈内动脉均来自右无名动脉,故右无名动脉对脑底供血十分重要;双侧有沿脊柱向尾侧走行的折返动脉;胸主动脉背侧由胸6至胸14平面共发出8条肋间动脉,为胸腰脊髓的主要供血动脉;无腰大动脉。

The thymuses, spleens, sera and anticoagulant blood were collected after the rats were killed, to carry out these works:(1) Zinc concentrations were analyzed by atomic absorption spectrophotometry;(2) Body weight and the weight of thymuses and spleens were measured, the organ index were calculated;(3) Changes in pathology of thymus and spleen were observed;(4) Using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thmocytes and spleen lymphocytes was checked;(5) The expression of bcl-2、bax mRNA in thymus and spleen were detected by RT-PCR (reverse transcription polymerase chain reaction);(6) The expression of p56〓, a signal transduction protein in thymus and spleen were detected by immunohistochemistry;(7) Two-color cytofluorometric analysis was used to assess the expression of CD4, CD8, CD45RA and CD45RC in peripheral blood lymphocytes.

动物处死后留组织、抗凝血及血清,进行以下检测:(1)原子吸收法测血清锌浓度;(2)测胸腺、脾脏脏器重量并与大鼠体重比较,计算脏器指数;(3)HE染色对胸腺、脾脏进行病理观察;(4)原位末端标记法测胸腺、脾脏中淋巴细胞的自发凋亡,并通过图像分析进行比较;(5)逆转录聚合酶链式反应检测凋亡调控基因bcl-2、bax mRNA在免疫器官中的表达;(6)免疫组化方法检测信号转导蛋白p56〓在免疫器官中的表达;(7)流式细胞术分析外周血淋巴细胞中CD4+、CD8+、CD45RA+及CD45RC+CD4+、CD45RC-CD4+细胞的数量和比例。

To study the effects on the body of animals after incorporation of 3H-TdR and its mechanism, the laws of distribution A retention and absorbed dose in blood , thymus, spleen, femur, heart, lung,kidney, liver, brain , spermary , small intestine and muscle were detected by liquid scintillation counter after 3H-TdR was injected into the tail vein of mouse; the process of dynamic incorporation of in the lymphocytic cell nucleus of blood , thymus , spleen and the nucleus of femur was monitored; the absorbed dose in the cell nucleus was estimated; the influence to RNA"s synthesizing and cell"s survival was observed using the technology of CLSM; A segment of Egr-1 gene was cloned with reverse transcriptase- polymerase chained reaction, the influence to the expression of Egr-1 gene in the spleen cells was observed.

为了探讨有机结合氚内污染对生物机体的影响及其作用机理,本课题应用均相液体闪烁测量技术研究了氚标记胸腺嘧啶核苷(~3H-TdR)经尾静脉注入小鼠机体后,在血液、脑、胸腺、心脏、肺脏、肝脏、脾脏、肾脏、小肠、睾丸、肌肉和股骨共12种组织器官的生物动力学分布规律及其吸收剂量;应用纸片法液体闪烁测量技术研究了~3H-TdR内污染在外周血、胸腺和脾脏淋巴细胞及骨髓细胞的动态滞留规律,估算了细胞核的吸收剂量;应用共聚焦显微镜观察了~3H-TdR内污染对脾细胞核酸合成及其存活率的作用,应用反转录PCR技术研究了~3H-TdR内污染对脾细胞早期生长反应基因-1(Egr-1)转录表达的影响。

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