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Evaluation of IEM103 in rabbit animal model.The evaluation was based on the control-challenge study (IEM101 as control). Rabbits were laparotomized, injecteded 10〓 cfu directly into proximal small bowel, and 28 days later, challenged with 0.25--2ug CT and 10〓-10〓 cfu 4 wild toxic strains with different biotypes and serotypes. Experimental group developed antitoxic and significant vibriocidal immune responses to vaccination, and could protect no less than 2ug CT and 10〓-10〓 cfu all wild strains challenge.

IEM103的免疫原性评价:经兔肠段结扎免疫〓cfu IEM103,7天后在外周血中可检测到外周淋巴细胞分泌性抗CT IgG,血清中可检测到抗CT抗体、杀弧菌抗体,P/N均≥2,且可持续至28天。28天后用0.25-2ug CT毒素,四种毒株滨-43,吴江-2,395,1119〓cfu菌量攻毒,实验组可保护至少2ug CT攻毒,而对照组可保护1ug以下CT攻毒,两组均可保护〓cfu四种毒株攻毒(以肠积液量≥1.0ml/cm为不保护判断标准)。

Methods: To made the rabbit model of virosis fever using Sendai virus,and treat them with niuhuang chengqi tang b...

采用仙台病毒气管注射建立兔病毒性发热模型,应用牛黄承气汤灌胃治疗,观察其解热效果和对脑肠组织及血浆中脑肠肽P物质和神经降压素及胸腺中CD4+、CD8+及外周血中γ干扰素的含量的影响。

The tissue samples of small intestine, liver and blood samples from peripheral and portal vein of 4 macaques of 6-month fetus, 2-day neonate, 45-day neonate and adult were obtained after anesthetization.

通过手术途径获得胚胎6月、新生2 d、新生45 d和成年猕猴的回肠、肝脏、门静脉和外周血等标本,应用放射免疫分析法测定各标本中的VIP含量;通过免疫组化方法观察VIP在肠、肝组织内的分布;利用原位杂交法检测VIP受体1(VIP receptor 1,VIPR1)的表达。

(1)Both the liver regeneration and apoptosis are influenced by conspicuous bilirubinemia, hepatectomy and hepatic artery resection in rats after obstructive jaundice, especially by the liver resection volume and conspicuous bilirubinemia.(2) Under the condition of conspicuous bilirubinemia, the influence of 70% PH with HAR on liver regeneration and apoptosis is severer than its counterpart of 42% in the rats. Thus, the former is not safe and should be avoided and the later can be used.

高胆红素血症时,肝切除量是影响大鼠肝切除联合肝动脉切除实施安全性的重要因素,42%肝切除联合肝固有动脉切除、胆肠再通内引流,对肝细胞再生和凋亡影响较小,安全可行;70%肝切除联合肝动脉切除、胆肠再通内引流后肝细胞再生显著受抑制,凋亡增多,死亡率高,应避免实施。

Las multiplied again in the hemocoel and then invaded other host tissues, including hemocytes, nervous tissue, fat body, trachea, and muscle. Eventually, Las invaded the salivary gland and the Las-infected psyllids were enabled to transmit Las to other host plants. Symptoms of serious pathogenesis occurred in the muscle tissue.

黄龙病菌必需先穿过中肠上皮细胞并於中肠细胞内进行增殖,接著进入血腔继续进行增殖,并逐渐移行至其他组织包括血球、神经、脂肪体、气管上皮细胞及肌肉等,最后抵达唾腺,届时柑橘木虱即具有传播黄龙病的能力。

There is plenty of polyphyly anastomosis of sural nerve's nutrient vessels and there will be better for offering the flood through the sural nerve's nutrient vessels design retrograde skin flap.

腓肠神经营养血管有多源性、吻合丰富的特点,以腓肠神经营养血管设计逆行皮瓣,血供较好,修复足部软组织缺损能获得较好效果。

There is plenty of polyphyly anastomosis of sural nerve's nutrient vessels and there will be better for offering the flood through the sural nerve's nutrient vessels design retrograde skin flap. We can get a better result through the way of repairing the lack of foot soft tissue.

腓肠神经营养血管有源性、吻合丰富的特点,以腓肠神经营养血管设计逆行皮瓣,血供较好,修复足部软组织缺损能获得较好效果。

Results The PGE2 levels in the portal vein increased, while the PGE2 levels in peripheral blood had no changes significantly, the PGE2 levels in the tissues of the intestinal mucosa, placenta, amnion and amniotic cells were increased significantly; Ricinoleic acid stimulated the synthesis of PGE2 in the above tissues invitro, which had the positive correlations with the dose of ricinoleic acid and its lasting time.

结果 实验组大鼠门静脉PGE2水平升高,外周血PGE2水平无变化;实验组大鼠肠粘膜、胎盘、羊膜组织和羊膜细胞PGE2合成明显升高;蓖麻酸刺激肠粘膜、胎盘、羊膜等组织和细胞PGE2合成,其刺激合成能力与蓖麻酸浓度及其作用时间呈正相关。

The liver cell cord was obvious,but there was no apparent acini hepatic and central veins,however,the hepatic sinusoid in liver was developed.

肝细胞索明显,但无明显的肝小叶,亦无明显的中央静脉存在,但肝血窦发达;胰腺主要弥散分布于肝脏中以及胃与肠交接之处,其他还伴随着肠呈纵行分布。

The total 48 Haemophilus parasuis strains isolated from Guangdong province in 2007-2008 were serotyped by Indirect Haemagglutination Assay, and respectively belonged to 7 serotypes, 5 serotype with 23.68%.

采用肠杆菌基因间重复序列-PCR(Enterobacterial repetitive intergenic consensus-PCR)对保存的2007~2008年广东地区发病猪群中分离的48株副猪嗜血杆菌进行了分析,用间接血凝进行血清分型,PHYLIP-3.68软件对电泳图谱进行基因分型及遗传关系的分析。

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这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。