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血细菌培养

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Totally 31 patients with bacteriologically or histologically confirmed extrapulmonary tuberculosis in Peking Union Medical College Hospital received T-SPOT.TB assay to detect early secreting antigen target 6 or culture filtrate protein 10 peptides-specific T cells in the peripheral blood mononuclear cells.

对北京协和医院根据细菌学或/和组织学诊断的肺外结核患者31例,应用酶联免疫斑点技术检测6kD早期分泌靶向抗原和10kD培养滤过蛋白肽段库刺激后外周血中释放γ-干扰素的特异性T细胞。

Simultaneously responsible pathogen was detected by analyses of BAL, sputum and blood specimen, with regard to cell differential count, cytopathologic examination and cultures for bacteria, fungi and viruses.

并通过支气管肺泡灌洗液、痰、咽拭子及血标本的检查进行病原学诊断,包括细胞分类、病理学检查及细菌、真菌和病毒的培养等。

A virus strain with high hemagglutinin titer was isolated out from embryonated egg and MDCK cells after they were inoculated with the lung tissues of the died marmosets.

在排除细菌感染的基础上,通过死亡狨猴肺组织匀浆接种鸡胚和MDCK细胞的分离培养,分离出一株具有很高血凝效价的病毒株。

A virus strain with high hemagglutinin titer was isolated out from embryonated eggs and MDCK cells after inoculating with the lung tissues suspension of the dead marmosets.

在排除细菌感染的基础上,通过死亡狨猴肺组织匀浆接种鸡胚和MDCK细胞的分离培养,分离出一株具有高血凝效价的病毒株。

The PCR based assay was tested on 19 strains isolated in throats from patients with guttate psoriasis. The results demonstrated that 6 strains were positive in 15 streptococus strains among which there were 3 streptococus pyogenes, 1.S.agalactiae, 1 S. eguisimilis and 1 S. uberis, and 4 non- streptococus strains were negative. Emm6 was found not only in suppurative hemolytic streptococus, but also in S.uberis.

应用聚合酶链式反应方法对点滴型银屑病患者咽部培养出的19株细菌中的M6蛋白基因进行检测,结果,15株链球菌中6株为阳性,其中化脓链球菌3株,无乳链球菌、似马链球菌和乳房链球菌各1株,4株非链球菌均为阴性。M6蛋白基因不仅存在化脓性溶血链球菌中,在其它链球菌如乳房链球菌中也存在。

Objective To study the relation of hemolytic culture and bacterial-L-form's test.

目的 研究溶血培养法与细菌L型检出之间的关系。

Objective To study the relation of hemolytic culture and bacterial-L-form's test.

目的 溶血培养法与细菌L型检出之间的关系。

Objective: To investigate the distribution and drug resistance of pathogenic bacteria in blood culture in order to provide evidence for the treatment of diseases in clinical practice.

目的:了解我院血培养中的细菌分布和耐药情况,为临床治疗提供依据。

If the results of blood culture proven bacterial infection, it should be fluid, probably will be good for 10 days.

如果血培养的结果证明有细菌感染,那就要输液,大概10天就能好。

Methods The purified general bacteria in broth culture for46hourswerepreserved in glycerol-physiological saline,the purified streptococcus and Neisseria scraped from the agar culture were preserved in fresh sheep-blood which was added suitable glycerol-physiological saline,the purified fungi were cultivated in half-solid culture;-20℃general bacteria and fungi,-80℃streptococcus and Neisseria.

将普通细菌的菌种纯化后接种于普通肉汤管,4~6h增菌后,加入适量甘油-生理盐水保存液,-20℃冰箱保存;链球菌属和奈瑟氏菌属的菌株纯化后,用接种环取菌置于新鲜脱纤维绵羊血中,加入适量保存液,-80℃低温冰箱保存;真菌菌种纯化后接种于带硅胶塞的半固体培养基,25℃培养24h后,-20℃冰箱保存。

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