血细菌培养

Retrospective investigation was used to analyses the correlation factors of bacteremia. The results of bacterial culture and drug susceptibility test of blood in our hospital in 2004 were analyzed.

采用回顾性调查，分析菌血症发生的相关因素，对2004年我院住院病人血液细菌培养和药敏试验结果进行分析。

Abstract］ objective to evaluate the effect of regulating bloodmobile sterilization management.methods making a statistical contrast to the number of bacterial in the air flow,on object surface in the bloodmobile car before and after regulating sterilization management.results before regulating management,the total excess rate of bacteria in the air is 41.67% while the rate of object surface is 20.83% in the car.after regulating management,the total excess rate of bacteria in the air is 4.17% while the rate of object surface is 0.00%.the differences were significant.conclusion to realize regulative and effective sterilization management,consolidate sterilization measures,guarantee the quality of blood and the safety of blood collection is of important significance.

摘要］ 目的评价规范流动采血车消毒灭菌管理的效果。方法对流动采血车内的空气、物表在规范消毒灭菌管理前后细菌培养数量的统计对比。结果规范管理前车内空气细菌总数超标率达41.67%，物表达20.83%。而规范管理后车内空气细菌超标率是4.17%，物表细菌超标率是0，差异有统计学意义。结论对流动采血车实施规范有效的消毒灭菌管理，务实消毒灭菌措施，对保证血液质量和采血安全具有重要的意义。

After washing, 5 ml of the blood was taken to culture, and 200～300 ml of the blood was taken to add with staphylococcus aureus, bacillus pyocyaneus and colon bacillus.

留取处理血液200～300 ml，从中取血样5 ml进行细菌培养，在余血中加入金黄色葡萄球菌、绿脓杆菌和大肠杆菌。

Methods From Feb 2006 to Jun 2006,188 hospitalized children in Shenzhen children s hospital, were collected deep tracheal aspirate at the time of hospitalization. The respiratory tract secretions were immediately sent for bacterial culture with 3 kinds of medium:ordinary medium, Hemophilus influenzae selective medium, Streptococcus penumoniae selective medium. Then we extracted the total nucleic acids from secretions, and detected Mycoplasma pneumoniae by single fluorescent quantitation PCR. Simultaneously, 14 respiratory tract pathogenic bacterium and Mycoplasma pneumoniae were detected by Target Enriched multiplex PCR. Amplification products were identified by the Luminex100 suspension array.

确诊为社区获得性肺炎的患儿188例，在入院当天采集深部呼吸道吸引物，用普通培养基和肺炎链球菌、流感嗜血杆菌选择性培养基进行细菌培养，然后提取深部呼吸道吸引物中病原体的DNA，采用荧光定量单PCR的方法检测肺炎支原体，并对同一标本采用靶序列富集多重PCR技术同时扩增肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、肺炎克雷伯菌、大肠杆菌、嗜肺军团菌、铜绿假单胞菌、鲍曼氏不动杆菌、脑膜炎奈瑟氏菌、阴沟肠杆菌、奇异变形杆菌、化脓链球菌、粪肠球菌及屎肠球菌14种呼吸道病原菌和肺炎支原体的靶基因，扩增产物用Luminex100多功能悬浮点阵仪检测。

For Streptococcus penumoniae、Hemophilus in0fluenzae、Staphylococcus aureus、Acinetobacter baumannii、Pseudomonas aeruginosa,the sensitivety of Tem-PCR was 100%、41.7 %、11.1%、50.0 %、66.7 % respectively; the specificity was 84.0%、81.7%、98.9%、95.2%、98.9% respectively. Tem-PCR was more sensitive than culture in the detection of Streptococcus penumoniae 36/19.1% vs.

3以细菌培养作为参考标准，Tem-PCR对14种病原菌总的敏感性为51.0%，特异性为68.0%，符合率为58.3%，对肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、鲍曼氏不动杆菌、铜绿假单胞菌的敏感性分别为100%、41.7%、11.1%、50.0%、66.7%，特异性分别为84.0%、81.7%、98.9%、95.2%、98.9%。

Methods Under aseptic condition, mechanically blocking of the hepatoduodenal ligement was adopted in rabbit model, both endotoxin level of the portal blood and positive bacterial cultures in the mensenteric lymph nodes were observed at 15, 20, 30 and 60 min respectively after the blocking.

采用兔动物模型，在无菌条件下机械性阻断肝十二指肠韧带，观察阻断后15，20，30min和60min时门静脉血中内毒素值和肠系膜淋巴结细菌培养阳性数。

RESULTS Sixty fungal strains were isolated from blood.Candida was the predominant pathogenic organism(86.7%),6 cases had mixed infection causing by two fungal species(11.1%).Twenty two cases had concomitant bacteremia(40.7%).Overall mortality rate was 68.5%,directly related mortality rate in treatment group was significantly lower than that in nontreatment one(28.9%vs.88.9%,χ2=11.268,P＜0.01).Effective rate of amphotericin B was 68.8%,fluconazole 70.8%,combined treatment 80.0%.

结果 54例患者血微生物培养共获得真菌菌株60株，念珠菌是主要病原体，占86.7%；6例(11.1%)为两种真菌混合感染；22例(40.7%)伴随细菌血症；总病死率68.5%，真菌血症相关病死率治疗组28.9%，未治疗组88.9%，两组比较χ2=11.268，P＜0.01；两性霉素B及氟康唑治疗有效率分别为68.8%和70.8%，两者联合治疗有效率为80.0%。

This research determines the change that analysed patient of mobile sex tuberculosis to treat around CRP, medium clinical import is evaluated in order to discuss CRP in phthisical curative effect. 1.1 average data choose 1 data and method to come from January 2005 August 2007 the patient of mobile sex tuberculosis of my courtyard be in hospital 120, all case of illness all combine the phlegmy smear, diagnose such as germiculture according to sternum of line of clinical expression, X or bosom CT, the tuberculosis that chooses case of illness to all accord with China cure to learn tuberculosis to learn branch to make is diagnosed and treat a guideline [2] , 120 all are patient of mobile sex tuberculosis is treated first, male 78, female 18 ～ is 73 42; age years old, average age (40 ± 6) year old. 1.2 methods are all patient early morning is hollow vein collects blood, the United States is used after detached serum elegant earth up Ci8200 full automatic biochemical analyzer determines, use scattering immunity to determine than chaotic law serum CRP level, and determine at the same time red blood cell sedimentations rate , bacterium of phlegmy n/med tuberculosis is checked of education of n/med tuberculosis bacterium...

本探究测定分析了活动性肺结核患者治疗前后CRP的变化，以探索CRP在肺结核疗效评估中的临床意义。1资料和方法1.1一般资料选自2005年1月至2007年8月我院住院的活动性肺结核患者120例，所有病例均根据临床表现、X线胸片或胸部CT结合痰涂片、细菌培养等确诊，所选病例均符合中华医学会结核病学会分会制定的肺结核诊断和治疗指南[2]，120例均为初治活动性肺结核患者，男78例，女42例；年龄18～73岁，平均年龄(40±6)岁。1.2方法所有患者清晨空腹静脉采血，分离血清后采用美国雅培Ci8200全自动生化分析仪测定，采用散射免疫比浊法测定血清CRP水平，且同时测定红细胞沉降率，痰结核菌检查。。。

Methods Biles of 120 cases and the blood samples of 40 of all patients with cholelithiasis along with biliary infection were collected and cultured respectively during endoscopic retrograde cholangiopancreatography by deep cannulation and suking out bile through the catheter.

方法对浙江省象山县第一人民医院2003-01~2004-05胆总管结石并胆道感染患者120例通过ERCP胆管深插管抽取胆汁进行细菌培养加药敏试验，同时取40例进行血培养。

Results 371 bp DNA fragment was amplified from 24 different species. The sensitivity could be improved to 10-12 g. No signal was observed when human DNA,funguses and viruses were used as templates. 22 blood samples and 4 cerebrospinal fluid samples, being positive with culture, were positive by using PCR. The gramnegative and grampositive probes hybridized to clinic samples and different species, as predicted by Gram stain characteristics.

结果 对24株不同标准菌株进行PCR扩增，均出现371 bp长度的DNA片段，敏感性试验可检测出10-12 g的细菌DNA，与人类基因组DNA、真菌及病毒无交叉反应；22例血培养阳性标本及4例脑脊液培养阳性标本均扩增出371 bp长度DNA条带，反相杂交法区分革兰阳性/阴性细菌与培养结果相符。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。