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血红蛋白测定法

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The reference values of 5429 Chinese healthy toddler boy's hemoglobin tested in 45 areas are collected and processed in order to explore the relationship between the reference value of Chinese toddler boy's hemoglobin and altitude by using curvilinear regression analysis.

收集了中国45个单位用氰化高铁血红蛋白法测定的5429例健康幼儿前期男性血红蛋白参考值,用曲线回归分析的方法,研究其与海拔高度之间的关系。

Methods: The reference values of 4221 Chinese healthy infant boy's hemoglobin tested in 46 areas are collected, a research is made about relationship between the reference value of Chinese infant boy's hemoglobin and altitude by using curvilinear regression analysis.

方法收集了中国46个单位用氰化高铁血红蛋白法测定的4221例健康男性婴儿血红蛋白参考值,用曲线回归分析的方法研究了其与海拔高度之间的关系。

While erthrocyte, hemoglobin and Hela cell were under oxidative damage induced by the hydrogen peroxide (H2O2) or tert-butyl hydroperoxide on different concentrations of dihydromyricetin, hemolysis rate of erythrocyte and methemoglobin converted from hemoglobin was estimated by ultraviolet-visible absorption spectroscopy. Whereas mmalondialdehyde from erythrocyte was measured by thiobarbituric acid colorimetry, the cellular viability of Hela cell was examined using MTT 3-(4, 5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide assay.

在不同的二氢杨梅素浓度下,用过氧化氢或过氧化特丁烷对红细胞、血红蛋白和Hela细胞进行氧化伤害,以紫外-可见光分光光度法检测红细胞溶血率和由正常血红蛋白生成的高铁血红蛋白,硫代巴比妥酸法测定红细胞中的丙二醛,MTT法检测Hela细胞的活力。

Methods Tissue inorganic nitrate,malonaldehyde and NOS activity of the heart,liver,brain and kidney were determinated respectively by Cu2+-activated cadmium-reduction method,thiobarbituric acid test and oxyhemoglobin oxidation method.

采用铜离子活化镉还原法、硫代巴比妥酸染色法和血红蛋白氧化法测定组织NO、丙二醛含量和NOS的活性。

Presented in this article is a report of synchronous determination of acetylcholinesterase and butyrylcholinesterase in finger blood established on the basis of the regression equation (Y = 0.149 8X+22.483 5) where hemoglobin is highly correlated with hematocrit (r = 0.999 2, P.001), with quinidine sulphate as the specific inhibitor of BChE.

本文根据全血的血红蛋白含量与红细胞压积容量的高度相关性的回归方程式(Y=0.149 8X+22.4835),以及用硫酸奎尼丁作为丁酰胆碱酯酶的特异性抑制剂,建立了指血乙酰胆碱酯酶和BChE活性的同步测定法。

This study was aimed to evaluate the effect of benzyl alcohol on trehaloseloading red blood cells. The RBCs were incubated in 10, 30, 50 and 100 mmol/L concentrations of benzyl alcoholtrehaloe solution at 4℃ for 24 hours. The hemolysis rate of loaded RBCs was detected by using cyanohemoglobin kit, the intracellular trehalose level were assayed by sulfate anthrone method.

为研究苯甲醇对海藻糖负载红细胞的影响,在4℃条件下将红细胞孵育在浓度分别为10、30、50、100 mmol/L的苯甲醇海藻糖溶液中24小时,用氰化血红蛋白试剂盒测定海藻糖负载红细胞的溶血率,用硫酸蒽酮法检测红细胞内海藻糖浓度水平。

The cyanmethemoglobin method recommended by WHO was used to detect hemoglobin, and the "Standard for Height and Body Weight of Chinese Students Aged 7~22 Years in 1995" was used to assess the nutritional status of these children.

对柳州市135所中小学校7~19岁学生80036名进行体格检查,采用WHO推荐的氰化高铁法测定血红蛋白和用《1995年全国学生7~22岁身高标准体重值》进行营养状况评价。

Hemoglobin values were determined by cyanmethemoglobin method according to the WHO1972 diagnostic criteria for anemia.

采用氰化高铁法测定血红蛋白值,诊断按WHO 1972年贫血筛查标准。

Methods:Thyroid function,thyroid microsome antibody,and thyroid globulin antibody were determined with radioimmunoassay in 23 CRF complicated by primary hypothyroidism patients,and the serum calcium,phosphorus,potassium, cholesterol, glucose, urea nitrogen, hemoglobulin,and endogenous creatinine clearance rate were also assayed.

用放射免疫法测定23例CRF合并甲减患者的甲状腺功能、甲状腺微粒体抗体、甲状腺球蛋白抗体及检测血清钙、血清磷、血清钾、血清胆固醇、血清肌酐、血糖、尿素氮、血红蛋白、内生肌酐清除率并与31例单纯CRF患者对照进行分析。

METHODS: Fasting serum levels of IL6, IL8, TNFα were measured in 30 cases of T2DM with insulin resistance and 30 cases of T2DM without insulin resistance and 26 normal control subjects by enzymelinked immunosorbent assay.

采用酶联免疫吸附法测定T2DM伴有IR患者30例和不伴有IR患者30例及正常对照26例的空腹血清白细胞介素6(IL6),白细胞介素8(IL8)及肿瘤坏死因子的浓度,并同时测定空腹血糖、糖化血红蛋白(GHbA1c)及空腹胰岛素水平,分析其与IL6,IL8,TNFα的相关性。

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