血红蛋白分光光度计

By addition of suitable amount of sodium hydrosulfite into blood samples which were diluted by 0.1% Na2CO3, the absorption curve from 400 nm to 600 nm and absorbance at 419 nm and 425.5 nm of the blood samples were detected for qualitative and quantitative determination of carboxyhemoglobin.

检血用0.1% Na2CO3稀释后加入连二亚硫酸钠，用紫外－可见分光光度计测定500nm-600nm吸收光谱图和419nm及425.5nm处的吸光度值，对碳氧血红蛋白进行定性和定量分析。

The high potassium ferrocyanide solution was added to CAM homogenates and the absorbance was detected by ultraviolet spectrophotometer. The hemoglobin content of CAM homogenates was obtained by reference standards.

分离发育期鸡胚尿囊膜并将其匀浆，加入高铁氰化钾溶液，用紫外分光光度计测量A值，通过标准品计算样品中血红蛋白的含量。

Methods: The specifications of ultraviolet-visible spectrophotometers wereaffirmed to ensure that they can meet the requirements of the reference method, andthe reference measurement procedure was established. According to the guidelines ofmethodology validation in ICH and AOAC, we chose the selectivity, linearity,precision and accuracy to evaluate. The linearity and precision were validatedaccording to NCCLS EP6-P2 and EP5-A.

确认分光光度计的性能可满足血红蛋白参考方法的要求，确定参考测量程序；参照美国临床实验室标准化委员会文件EP6-P2和EP5-A评价该方法的线性和精密度，通过测定WHO国际标准物质验证我室血红蛋白参考方法测量结果的准确性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。