血红蛋白

METHOD:Porcine hemoglobin with high purity and activity was prepared by centrifuging,micro filtration and weak anion exchange chromatography.

方法通过离心、微滤和弱阴离子交换层析法，制备高纯度高活性的猪血红蛋白，并建立合理的检验方法(紫外可见光扫描、电泳、高效液相色谱、血凝试验抑制试验和血气分析等)，以确定纯化产品的含量、纯度和活性。

In the present study, four derivatives of PEG with different activation groups, and several PEGs with different molecular weights were covalently bound to porcine hemoglobin.

比较了4种方法活化的PEG衍生物对猪血红蛋白的修饰效率、修饰产物的携氧功能和稳定性等。

The laboratory research and chinical trials to prove.courage lcecontains active material that it can quickly penetrate through body's cell.so to directly provides for the cell,fill the skin intercellular space the crevice which is senile withered because of the cell stays benind supply the active elements necessary for the body cells metabolism,to activate the function of endothelium cells of blood vessels,promote the blood vessels balance, compose and secrete various blood vessels active elements,contains abundantsulfuric cartilage element,sulfuric amido etc.,all of fatty acid in human body.and it can accelerete the re-generation of blood cells,add the quantity of the hemoglobin and red blood corpuscle so to improve the blood parameter,and fundamentally improve the body cells metabolism and nutrition supply for all the organs,as a result,you will thoroughly refresh yourself and bloom vitality of youth.

实验室研究和临床试验证明，LCE所含活性物质能够迅速穿透人体细胞，直接为细胞供养，填补肌肤细胞间因细胞衰老干瘪而留下的空隙全面补充人体细胞新陈代谢所必须的活性物质，激活血管内皮细胞功能，促进血管平衡，合成和分泌多种血管活性物质：富含的硫酸软骨素、硫酸氨基葡萄糖等能改善和调节人体脂代谢，有效清除体内血液中多余的脂质和脂蛋白清除在血管壁内膜沉积的脂类物质，保持人体脂肪酸均衡：能够加快血液细胞更新，提高血液中的血红蛋白和红血球数量，改善血液参量，从根本上改善人体细胞的新陈代谢，改善人体各组织器官的营养供给，使人由内而外脱胎换骨，重新焕发青春活力。

The laboratory research and chinical trials to prove.courage lcecontains active material that it can quickly penetrate through body's cell.so to directly provides for the cell,fill the skin intercellular space the crevice which is senile withered because of the cell stays benind supply the active elements necessary for the body cells metabolism,to activate the function of endothelium cells of blood vessels,promote the blood vessels balance, compose and secrete various blood vessels active elements,contains abundantsulfuric cartilage element,sulfuric amido etc.,all of fatty acid in human body.and it can accelerete the re-generation of blood cells,add the quantity of the hemoglobin and red blood corpuscle so to improve the blood parameter,and fundamentally improve the body cells metabolism and nutrition supply for all the organs,as a result,you will thoroughly refresh yourself and bloom vitality of youth.

实验室研究和临床试验证明，LCE所含活性物质能够迅速穿透人体细胞，直接为细胞供养，填补肌肤细胞间因细胞衰老干瘪而留下的空隙全面补充人体细胞新陈代谢所必须的活性物质，激活血管内皮细胞功能，促进血管平衡，合成和分泌多种血管活性物质：富含的硫酸软骨素、硫酸氨基葡萄糖等能改善和调节人体脂代谢，有效清除体内血液中多余的脂质和脂蛋白清除在血管壁内膜沉积的脂类物质，保持人体脂肪酸均衡：能够加快血液细胞更新，提高血液中的血红蛋白和红血球数量，改善血液参量，从根本上改善人体细胞的新陈代谢，改善人体各组织***的营养供给，使人由内而外脱胎换骨，重新焕发青春活力。

During support with the PUCA pump, mean arterial pressure, systolic blood pressure and diastolic blood pressure increased gradually and close to the baseline at the end of experiment. No significant change in platelet and plasma free hemoglobin were found during the whole period of the PUCA pump support.

成功的7只绵羊在支持期间血流动力学逐步恢复并趋向稳定，血压接近基线值；血小板和血浆游离血红蛋白在辅助期间无明显变化；肾、隔膜泵及导管均未见明显的血栓形成。

Methods After heart failure model was induced successfully in 10 sheep, PUCA pump was inserted through aorta and the aortic valve orifice with its tip located in the left ventricle. It was then activated to support the hemodynamics for 3 hours. Hemodynamic parameters were monitored and recorded before thoracotomy, heart failure, and every 60 min after the support. Platelet and plasma free hemoglobin were tested during the experiment.

方法对10只绵羊诱导左心衰竭模型建立成功后行PUCA泵辅助3h，监测并记录血流动力学参数，采动脉血人工计数血小板并测定血浆游离血红蛋白；经辅助3h后检查肾、隔膜泵及导管血栓形成的情况。

The results showed that the hypoglycaemic and the urine glucose lowering effect of PCE-C in alloxan-diabetes rabbits were excellently and long-lasting. PCE-C could also reduce the serum total cholesterol and the serum triglyceride in experimental rabbits to normal level, and delay the loss of body weight of the rabbits. The glycosylated hemoglobin is the true index that reflects the long-term controlling effect for diabetes, and the experiment result affirmed the veracity and reliability of the results of the blood glucose.

结果表明，PCE-C对实验家兔血糖和尿糖的降低效果具有高效性和持续性；当血糖控制不良时，可并发血脂水平的升高，PCE-C可以使血清总胆固醇和甘油三酯恢复正常水平，同时能减缓实验家兔的体重下降；糖化血红蛋白是真正反映糖尿病长期控制效果的指标，PCE-C对实验家兔GHb的作用影响充分证实了血糖测定结果的准确性和可靠性。

Free haemoglobin in plasma is bound by haptoglobin with high affinity. The complex is internalised by monocytes/macrophages and degraded.

血浆中的游离血红蛋白与结合珠蛋白紧密结合，这种复合物被单核细胞/巨噬细胞吞噬并降解。

Methaemoglobin loses its ability to bind oxygen and causes hypoxia in the affected individuals. The condition is characterized by headache, weakness and breathlessness, and a bluish discolouration of the skin and mucous membranes called cyanosis.

这是因为亚硝酸盐使体内红血球素中的铁由亚铁氧化成正铁，正铁血红蛋白失去携氧能力，造成血氧过少，令人感到头痛，虚弱，呼吸困难，皮肤和黏膜会变成紫蓝色，医学上称为发绀。

The main work and corresponding conclusions are outlined as follow:1. The character of Hb on a Lauric Acid modified GC electrode was analyzed by the method of CV, which results show that the electrochemical deoxidization of Hb on modified electrode was an irreversible charge transfer system.

本论文研究了血红蛋白在玻碳修饰电极上的电化学行为，并对所得实验测试信息进行了较为详细的分析，主要的研究结果如下： 1。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。