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(1) cerebral ischemical reperfusion injury rats'limbs motor function is variable. Acupuncture could promote lims'functional recovery.(2) PCNA masc cells is visible in cerebral ischemical semidarkness region. There is cell regeneration phenomenon. Acupuncture could strengthen injury region's PCNA expression, could profit injury recovery and functional reconstruction.(3) In ischemia semidarkness region for the model group and acupuncture group, PCNA masc cells percentage of 14days group is lower than 7days group. Along with the recovery of injury, cell multiplication is weaken.(4) In cerebral ischemia semidarkness region, there is VEGF masc cells and regeneration phenomenon. Acupuncture could strengthen injury region's VEGF expression, could profit protection after injury and blood vessel regenerate.(5) In ischemia semidarkness region for the model group and acupuncture group, VEGF masc cells percentage of 14days group is lower than 7days group. Along with the recovery of semidarkness region, ischemia and anoxemia state is getting improved, and VEGF is reduce.(6) As there are PCNA and VEGF masc cells in brain injured region, we could conclude that, after brain ischemical reperfusion injury, there are blood vessel regeneration phenomenon. Acupuncture could promote blood vessel regeneration, recovery blood supply sufficiently and quickly, and promote the recovery of brain injury region.(7)The VEGF masc cells percentage of inhibitor group is lower than acupuncture group. It state that the effect of acupuncture promote VEGF is partly depend on the existing of eNOS.

实验结论:(1)脑缺血再灌注损伤后大鼠的肢体运动功能发生改变,针刺可以促进肢体功能恢复;(2)脑缺损伤区可见PCNA阳性细胞,存在细胞再生现象,针刺可以增强损伤区PCNA的表达,有利于损伤的修复和功能重建;(3)针刺组和模型组14d时缺血损伤区PCNA阳性细胞百分比低于7d组,随着损伤逐渐得到修复,细胞增殖现象减弱;(4)脑缺血损伤区可见VEGF阳性细胞,存在内皮型细胞再生现象,针刺可以增强损伤区VEGF的表达,有利于脑损伤后保护和缺血区血管再生;(5)针刺组和模型组14d时缺血损伤区VEGF阳性细胞百分比低于7d组,随着缺血损伤的修复,缺血缺氧状态得到改善,产生的VEGF减少;(6)由于脑损伤区同时出现PCNA阳性细胞和VEGF阳性细胞,前者是增殖细胞的标志,后者是促进血管再生的重要因子,可以推断,脑缺血再灌注损伤后脑内存在血管再生现象,针刺可以促进损伤区的血管再生,更迅速而充分的恢复损伤区的血供,促进脑损伤区的修复;(7)抑制剂针刺组脑损伤区VEGF阳性细胞百分比与针刺组相比有不同程度的降低,说明针刺促进缺血损伤区VEGF表达部分依赖eNOS的存在。

Areas of its research include Applied Exercise Science, Cell Physiology, Comparative Physiology of Exercise, Developmental Neurobiology, Ecophysiology, Environmental and Comparative Endocrinology, Exercise Biochemistry, Human Cardiovascular, Integrative Vascular Biology, Locomotion, Motor Behavior, Neural Control of Movement, Neuroimmunophysiology, Reproductive Endocrinology, Sleep and Chronobiology.

其研究领域包括应用锻炼科学、细胞生理学、比较锻炼生理学、发育神经生物学、生态生理学、环境和比较内分泌学、锻炼生物化学、人类心血管学、综合血管生物学、运动、运动行为、运动的神经控制、神经免疫生理学、生殖内分泌学、睡眠以及时间生物学等。

This study evaluated the effect of two-month Tai Chi Chuan training on the brachial artery distensibility and compliance in the elder adults. Fifteen TCC practitioners and fifteen TCC trainees were recruited in this study.

目的:血管硬化是当今科学界研究老化的重要生理指标之一,本研究之目的正是探讨太极拳运动对於中老年人肱动脉血管弹性度的效应。

Six cadavers were prepared for necroscopy, operative procedure of ILIF were simulated on L3-S1, and all related important anatomic structure and their relationship were observed and record. ILIF with transpedicle screw fixation were also performed with cage insertion.3. Twelve fresh frozen bovine lumbar functional spinal units were prepared for biomechanical testing. According to the different treatment order, the specimens were devided into 7 groups, which included: 1intact specimens, 2specimens were treated by left unilateral facetectomy and had homolateral anatomical threaded cages inserted, 3 TLIF with homolateral pedicle screw fixation, 4 TLIF with bilateral pedicle screw fixation, 5 specimens had anatomical threaded cages inserted on the left by intertransverse process approach, 6 ILIF with homolateral pedicle screw fixation, 7 ILIF with bilateral pedicle screw fixation.

方法1、对20名正常志愿者L3-S1节段进行CT增强+三维重建检查,在三维重建图形上测量横突长度、上下横突间距、椎间孔外椎间隙高度及斜径,观查横突间区域血管走形特点并测量血管内径,然后进行统计分析及评价。2、采用福尔马林固定尸体标本六具,模拟ILIF手术对L3-S1节段进行操作,观察横突间区域重要结构的位置和毗邻关系,并模拟同侧椎弓根钉和单枚椎间融合器的置入过程,探讨该术式存在的问题并改进。3、采用小牛脊柱运动节段标本12具,依序进行不同处理后分为以下7组:⑴正常对照组CG⑵左侧小关节切除+椎间融合器植入组(TLIF⑶TLIF附加同侧椎弓根钉固定组;⑷TLIF附加双侧椎弓根钉固定组;⑸左侧横突间入路椎间融合器植入组ILIF⑹ILIF附加同侧椎弓根钉固定组;⑺ILIF附加双侧椎弓根钉固定组。

Objective: To observe the motor function variation of focal cerebral ischemical reperfusion injury rats'limbs at different time. To observe the expression of PCNA and VEGF. To observe the effect to that index when electric acupuncture the"Quchi"and"Housanli". To observe the effect to the focal cerebral ischemical reperfusion injury rats, which are inject eNOS catastaltic, when electric acupuncture that two acupoints. Through observing of above all things , we study acupuncture's blood vessel regenerated effect to cerebral ischemia reperfusion injury region, approach the possibility of brain functional recovery angiogenesis which promoted by acupuncture, provide new consider and experiment accordings for deepening acupuncture therapy of the ischemia stroke.

研究目的:通过观察局灶性脑缺血再灌注损伤大鼠不同时间点肢体运动功能的变化及损伤脑组织核心增长蛋白、血管内皮细胞生长因子的表达情况,电针&曲池&、&后三里&穴对以上各指标的影响、电针&曲池&、&后三里&对给予eNOS抑制剂对局灶性脑缺血再灌注损伤大鼠的影响,研究针刺对大鼠脑缺血再灌注损伤区血管再生的作用,探讨针刺促进脑功能恢复的血管生成可能机制,为进一步深化针灸治疗缺血性中风的认识提供新的思路和实验依据。

On the base that has mensurable estimation to hemal three-dimensional motion, extraction gives important athletic parameter, the amplitude that is like athletic way, displacement and athletic contrail.

在对血管的三维运动进行定量估计的基础上,提取出重要的运动参数,如运动方向、位移的幅值和运动轨迹等。

Average following period was 7.6±3.9 months;EF was 63%±5%;The movement of infarction-related myocardial wall was completely normal in 7 of 9 patients and 2 patients only had slight hypokinesis;Q waves on electrocardialgram was decreased (3/9) or disappeared (6/9),Free-symptom living was 8 patients,only one patient had some tightness on his chest when he did moderate exercis.

除术中、术后各1例并发上消化道大出血外,术中术后无明显心血管并发症(No-Reflow现象、急性血管闭塞、死亡、搭桥术、靶血管再介入治疗)发生。平均随访7.6±3.9月,7例梗塞相关室壁运动完全恢复;Q波导联减少3例,消失6例;EF为63%±5%。

It is suggested that these nerves may participate in the relaxation and constriction of the nonvascular smooth muscles. Perivascular nerve fibers in the genital organs may play an im-ortant role in the regulation of the uterine vascular tone.

分析雌性大鼠生殖器官内一氧化氮合酶Ⅰ免疫反应阳性神经纤维的分布,提示一氧化氮可能与血管活性或非血管平滑肌运动调节有关。

The above-mentioned results illustrate: the mechanism of pseudobulbar palsy treated with nape needle was possible about brain plasticity after stroke, for example, it could relieve spasm of muscle, recovery muscle strength, facilitate pharyngeal muscle to move coordinated; formate long term protention with uninterrupt mechamism stimulations , reconstructe damaged reflex arch ; accelerate blood circulation, ameliorate ischemia and anoxia; influence metabolism of angeogenesis growth factors, improve angiogenesis and protect neurons.

项针治疗脑卒中后假性延髓麻痹的疗效肯定,其机理可能主要与缓解肌肉痉挛,恢复肌力,促进咽部肌肉的协调运动;对病变部位长期不断的机械刺激,形成长期强化作用,使受损反射弧得以重建;促进血液循环,改善病变局部缺血缺氧状态;影响血管生成生长因子的代谢,促进血管生成,保护神经元等,脑卒中后脑可塑性改变有关。

The expressions of VEGF in rats' skeletal muscles induced by acute exercise and/or hypoxia belong to the effect of immediate-early, with existing intensity-threshold, which recovery speed is inversely proportional to the expression amplitude;"training low-living high" may be able to enhance the adaptation of skeletal muscles to sports.

急性运动和/或低氧诱导的大鼠骨骼肌血管内皮生长因子的表达属早期速发效应,且存在强度阈值,其恢复速度与表达的幅度成反比,低练高住可能能增强骨骼肌对运动的适应。关键词:血管内皮细胞生长因子;低氧;低练高住;运动强度

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