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Experiments show that Ang Ⅱ activates JNK mediated by AT〓, and JNK increases transcription activity of c-Jun by phosphorylating c-Jun amido-terminal, and induces expression of c-Fos gene. c-Jun and c-Fos form transcriptional factor activated protein-1 complex. AP-1 may stimulates genes which correlate with cardiovascular remodeling expression, such as transforming growth factor, collagen type Ⅰ and Ⅲ, skeletal muscle α-actin,β-MHC, atrial natriuretic factor.

实验表明,在心肌和血管平滑肌细胞Ang Ⅱ通过AT〓介导激活JNK;JNK通过磷酸化c-Jun氨基末端增加c-Jun的转录活性并诱导c-Fos基因表达,c-Jun和c-Fos蛋白形成转录因子复合物激活蛋白-1(AP-1),并刺激各种心血管重塑相关基因如转化生长因子-β1(TGF-β1)、Ⅰ,Ⅲ型胶原、骨骼肌α-actin、β-MHC(β-myosin heavy chain,β-肌球蛋白重链)、心钠素的表达。

Methods: A dual-chamber culture system was prepared. Porcine ovarian follicles were divided into healthy and atretic groups. Granulosa and thecal cells were isolated, and cultured with gonadotropin in the dual-chamber system. After 48 hours, angiotensin II, renin activity, estrodiol(E2), progesterone and testosterone in the media were determined by radioimmunoassay.

制备人胎盘羊膜双池培养系统,分离了猪卵巢颗粒细胞和卵泡膜细胞,并分为健康和闭锁两组,在促性腺激素刺激下协同培养。48 h后应用放射免疫测定法测定两种细胞培养液中的血管紧张素II、肾素活性、雌二醇(E2)、孕酮以及睾酮水平。

Fifteen patients undergoing hysteromyomectomy with 0 75% bupivacaine epidural anesthesia were studied to observe the alterations of the levels of plasma atral natriuretic peptide, renin activity, angistensin Ⅱ and aldosterone systems.

研究了15例子宫肌瘤切除术患者应用0.75%布比卡因硬膜外麻醉血浆心钠素和肾素活性、血管紧张素Ⅱ-醛固酮系统的变化及相互调控。结果表明,血浆ANP水平在麻醉10min后各时点明显低于麻醉前(P<0.05),PRA在麻醉10min后亦明显低于麻醉前(P<0.01),而AⅡ和AL无显著改变(P>0.05)。

Arsenic poisoning have magma role to paralysis small blood vessels,mercapto Jimei inhibit the activity of the liver and fat necrosischange Hepatic Lobules Centre, heart, liver, kidney, intestinecongestion, epithelial cell necrosis, telangiectasia.

砒霜有原浆毒作用,能麻痹细血管,抑制巯基梅的活性,并使肝脏脂变肝小叶中心坏死,心、肝、肾、肠充血,上皮细胞坏死,毛细血管扩张。

Arsenic poisoning have magma role to paralysis small blood vessels, mercapto Jimei inh ibit the activity of the liver and fat necrosis change Hepatic Lobules Centre, heart, liver, kidney, intestine congestion, epithelial cell necrosis, telangiectasia.

砒霜有原浆毒作用,能麻痹细血管,抑制巯基梅的活性,并使肝脏脂变肝小叶中心坏死,心、肝、肾、肠充血,上皮细胞坏死,毛细血管扩张。

Objective: To investigate the anti-angiogensis activity of naja naja venom.

目的:研究眼镜蛇毒在抗血管生成方面的生物活性。

The ACE activity in the arterial blood increased 30 rain after injection and declined 45 min later.

在血清和肺淋巴液ACE活性变化的同时,肺淋巴流量和肺跨血管蛋白清除率增加2~3倍。

Samples of N-methyl-2-arylbenzothiazolines (1) and 11 dimmers (2) were evaluated in vitro VEGF inhibitory activity in human breast cancer cell MDA-MB-231 and most of them showed potent VEGF inhibitory activity with the EC50 values of sub-millimolar range.

这些新的化合物(1)和(2)进行了体外人乳腺癌细胞(MDA-MB-231)血管内皮生长因子靶点筛选,大部分化合物都表现出了抑制活性。

The fact that urokinase can hydrolyse plasminogen to generate angiostatin without the participation of free sulfhydryl donor L-Cys suggests that there might be another pathway of angiostatin generation, in which urokinase hydrolyse plasminogen to generate angiostatin directly.

我们还比较了pH7.4、pH8.0、pH9.0三种pH条件下尿激酶酶切纤溶酶原生成血管抑素的反应结果,发现酶切活性在pH9.0时速度最大,pH8.0时反应速度最慢,pH7.4时反应速度介于二者之间,由此表明pH对上述酶切反应速度确实存在影响。

Then the recombinant retroviruses were used to infect endothelial cells and NIH3T3 cells.

分别经ELISA和MTT法测定TNF-αD11a在血管内皮细胞中的表达及其生物学活性。

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