血管活性的

AIM: To explore the role of poly-polymerase in the cultured endothelial cell apoptosis induced by angiotensin Ⅱ.

目的： 探讨体外AngⅡ在引起血管内皮细胞凋亡过程中细胞内多聚聚合酶的活性变化情况。

Epithelization of control group appeared earlier and faster than experimental group, wound healing rate of experimental group were decreased significantly at 2nd and 3rd week（P＜0.05）as well. The cell proliferation activity of wounds surface were higher in dermis and epidermis.

分离并切断SD大鼠胸9～腰1右侧脊神经，造模后在背部对称制作两个直径为1.0 cm的圆形创面，分别观察两侧创面愈合时间、创面大体和组织形态学、创面细胞增殖活性、创面修复过程中血管再生情况，并对数据进行统计学分析。

Isoflavone can increase cooperativity of adenosine, reduce blood vessel resistance, increase blood flow; and restrain blood platelet agglutination, decrease cell factor, active endogenesis fibrinolysin decrease Catecholamine chroma to improve cochlea microcirculation.

异黄酮可协同腺苷的作用，降低血管阻力，增加血流量；同时也能有效抗血小板聚集，抑制其细胞因子释放，促进内在纤溶酶活性，亦降低血浆儿茶酚胺浓度作用，进一步改善耳蜗微循环。

The protein expressed in the form of inclusion body must be renatured to gain its biological activity.

为研制开发rhK1-3，本实验通过建立体内、体外血管生成模型来检测rhK1-3的生物学活性。

The design, synthesis, and angiotensin Ⅱ antagonist activities of several quinazolinone and quinazoline phenoxyphenylacetic acids are described.

本文设计、合成了一系列喹唑啉酮和喹唑啉苯氧基苯乙酸类化合物，并检测了它们的血管紧张素Ⅱ受体拮抗活性。

Plasma renin activity is a powerful risk factor for vascular and kidney dysfunction.

血浆肾素活性是血管和肾脏功能不全的一个强烈危险因素。

High magnetic filter: Use KLQ unique high magnetic field E + E, complexification of magnet technology, so that has been activated small molecules of water with stronger activity, more time to maintain the water activity that was called not reducing property small molecules of water , constant magnetic field of 10,000 gauss systems, instantaneous release of 9600-10300 gauss of magnetic field lines, so that an orderly arrangement of small molecules of water take of various trace elements needed by human body,delay cell senescence, activation of T cell growth, modulation of human blood fat, improve body microcirculation, reducing blood vessel wall fat deposition, improve immunity, have a preventive of hyperlipidemia and other kinds of chronic diseases, diabetes, high blood pressure.

高磁化过滤器：使用KLQ独特的E+E高磁场，复化磁钢技术，使已经活化的小分子团水活性更强，具有磁记忆能，更长时间维持水的活性被称为不还原的小分子团水，10000高斯生物恒磁场系统，瞬间释放9600-10300高斯的磁力线，使小分子团有序排列摄取人体所需的各种微量元素，延缓细胞衰老，激活T细胞生长，调节人体血脂，改善身体微循环，减少血管壁脂肪沉积，提高免疫能力，可保健肝胃具有预防高血脂高血压糖尿病等种慢性疾病。

The expression of AT2R gene can be regulated efficiently in vivo by local delivery of MSC with dual-stable expression of AT2R gene, thus reduces the formation of intimal hyperplasia.④AT2R gene can influence the expression of ECM and significantly promote the cellular apoptosis in the injury rat carotid artery.⑤These data demonstrate the clinical potential of AT2R regulatable expression to prevent restenosis after PCI.

研究结论：(1)与VSMC直接接触可以诱导MSC向血管成分细胞分化；(2)构建的Dox-on可调控的MSC细胞株诱导活性可靠，使外源AT2R基因的表达处于有效的主动控制下；(3)局部导入MSC不影响球囊损伤后大鼠颈动脉新生内膜增生；(4)由MSC介导的AT2R可调控表达受到Dox的良好调控，可有效抑制球囊损伤后大鼠颈动脉新生内膜增生；(5)AT2R影响血管损伤后修复时ECM成分的表达，并促进细胞凋亡的发生，可能是AT2R介导抑制新生内膜形成的机制。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

In the previous studies the constituents from Cinnamomum philippinense reported to have the biological activities such as antiplatelet aggregation, vasorelaxation, cardiac muscle contraction, antioxidation and free radical scavenger activities etc. Furthermore, MA-1 a ligan isolated from Cinnamomum philippinense is considered to be an important prophylaxis to treat ischemic stroke due to its antioxidation and free-radical scavenger activities. The present project aims to develop MA-1 as a pre-medicine to treat ischemic stroke.

在过去的研究中，发现菲律宾樟树所含的成分对心脑血管相关的活性诸如抑制血小板凝集、血管松弛、影响心肌收缩、抗氧化、自由基清除等具有不错的活性作用，而由菲律宾樟树所分离得到的一种新木质酚MA-1，具有抗氧化作用且是自由基捕捉剂，获选为开发成缺血性中风之预防与治疗先导药物。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。