血管形成的

To observe method of chick embryo allantois membrane, judge neovascularisation, research the effect of angiogenesis by drugs by means of chick embryo allantois membrane model.

学会观察鸡胚尿囊膜血管的方法，判断新生血管形成，通过鸡胚尿囊膜模型探讨药物对血管生成作用的影响

(1) No blood vessels were found to differentiate and become the lymphatics in the mesentery of fistar rat, and this suggested that the lymphatic might develop from the mesenchymal cells in the tissue. Our results showed the mesenteric lymphatics developed """"""""centripetally"""""""".

结论(1)没有发现肠系膜血管分化形成淋巴管，淋巴管可能发生于血管以外的间充质细胞，即"向心"性发生。

Mallory trichrism staining exhibited light blue samples. Eight weeks following repair, CT showed round blunt defect edges in the coronal position, which had bony pustute connection with gel materials. Density at the defect region significantly increased. 3D reconstruction suggested that defects became small. After hematoxylin-eosin staining, abundant fibrous connective tissue appeared in defect regions, with bony tissues. Mallory trichrism staining revealed that maroon mature bone tissues were found, surrounded by light blue chondroid tissues. Twelve weeks following repair, coronal defect regions were filled with callus. 3D reconstruction showed that defects were repaired. Defect region and surrounding bony tissues had bony connection, with thick bone trabecula and mature Haversian system.

修复后8周，冠状位CT显示缺损边缘圆钝，与凝胶材料有骨性突起连接，缺损处密度增高明显，三维重建显示缺损范围较之前有所减小；标本苏木精－伊红染色后缺损部可见大量含血管成分的纤维结缔组织，有骨样组织结构形成，Mallory三色染色显示有褐红色成熟骨组织形成，其旁边有淡蓝色软骨样组织修复后12周，冠状位CT显示缺损区基本被骨痂填满，三维重建示缺损基本修复；缺损区域和周围骨组织形成骨性结合，骨小梁粗大，哈弗氏系统成熟。

Elastic fibers were well-distributed,and vascular endothelial cell did not immigrate.We suggested that expression of ectogenic VEGF triggered paracrine and autocrine of VEGF of chondrocyte and co-acted with VEGF receptor 2 to enhance permeability of chondrocyte and improve internal construct of engineering cartilage,and prevent vascularize proceed.

转VEGF基因软骨细胞作为组织工程的种子细胞与pluronic F-127复合后可于裸鼠体内形成转基因组织工程软骨，与对照组相比，转VEGF基因组织工程软骨具良好的生物学特性，结构均一且与正常软骨组织相似，软骨ECM的GAG、COLⅡ、COLⅩ增多，RunX2、Sox9表达增高，细胞处于增生期的肥大状态，初步分析其原因可能是转染后外源性的VEGF持续表达触发了软骨细胞VEGF自分泌，并通过VEGFR-2作用于软骨细胞，提高了软骨细胞活性，促进其存活与增殖，但未在软骨组织内引起血管内皮细胞的迁移及小血管形成。

Recent study pointed out:vascular endotheliocyte andangiogenesis have an important role in hemangiomas formation.

近年来的研究表明，血管内皮细胞和血管形成在血管瘤中起重要作用。

Topography map of the hip bone displays the depth of all parts of the hip bone and the projection of important blood vessel and nerve onto the hip bone imaginarily and directly.

将坐标系、等厚线和重要血管神经的投影图同时绘制在髋骨上，形成3张地形图，1张为髋骨内侧面地形图，1张为没有血管和神经结构的髋骨外侧面地形图，1张为有血管和神经结构的髋骨外侧面地形图。

PartⅢExperimental study of autologous BM-MNCs by intramyocardial transplantation into ischemic myocardium of AMI in swines Objective: To evaluate the effectiveness and safety of BM-MNCs by intramyocardial transplantation. Methods: 11 swines were divided into two groups: BM-MNCs group (n=6) and control group (n=5), total 2×10~8 cells.

实验动物分为心肌内移植MNCs组(n=6)及心肌内盐水对照组(n=5)，心肌内移植细胞数2×10~8；移植后4周观察移植细胞归巢情况、小血管密度、心功能及冠脉侧枝血管形成情况及可能发生的副作用。

DAPI can label the nucleus of MSCs effectively and has no obvious influence on the morphous and growth of MSCs.

应用MSCs+中药移植与MSCs组均可以促进血管新生、侧枝血管形成和增加缺血组织的血流灌注。

VEGF and the classification that its accept put oneself in another's position and action mechanism VEGF say blood-vessel is connected again appear factor (Vascular Permeabilityfactor, VPF) or blood-vessel are opsonic , it is right blood-vessel forms those who have main effect to grow factor.

VEGF及其受体的分类和功能机制VEGF又称血管通透因子(vascular permeabilityfactor，VPF)或血管调理素，是对血管形成具有重要功能的生长因子。

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色：翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成；基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质，在翼状胬肉体部深层基质中存在粗糙的纤维组织；正常球结膜组织细胞排列整齐；基质为疏松结缔组织，胶原纤维平行排列，其间可见成纤维细胞，散在少量中性粒细胞、毛细血管；Masson染色：翼状胬肉浅层基质中存在致密的胶原纤维染色，深层基质中的胶原纤维存在变性样改变；VG染色：翼状胬肉组织深层基质中存在大量变性的胶原纤维，其间夹杂黑色的弹性纤维；免疫组化染色法：MMP-3在翼状胬肉上皮细胞中呈强表达，成纤维细胞中呈中等强度表达，血管内皮细胞中未见表达；LN在血管壁中呈强表达，在上皮细胞基底膜中呈中等强度表达，在整个基质中未见明显表达；col Ⅲ在整个翼状胬肉基质中呈强表达。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。