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In order to definitude the effect of CGRP on diabetic angiopathy , we studied the function of CGRP in different concentration on proliferation and cell cycle of diabetic rat VSMC compared with normal control.

为明确CGRP在糖尿病血管并发症中的作用,本文研究了不同浓度CGRP对培养的糖尿病大鼠和正常大鼠VSMC的增殖和细胞周期的作用。

The purpose of this study was to investigate the vasorelaxing effect of vasonatrin peptide on human intramammary artery.The vasorelaxing effect of VNP on HIMA was measured by means of perfusion in vitro. The effects of HS-142-1, TEA, 8-Br-cGMP and methylene blue were also observed.

为了研究血管钠肽对人乳内动脉(human intramammary artery, HIMA)的舒张作用及其机制,采用离体血管灌流的方法,观察VNP对内皮完整和去内皮HIMA的舒张作用,以及HS-142-1、TEA、8-Br-cGMP和镁蓝对这一过程的影响。

We compared the spectral and its secondary structure of endostatin with and without of zinc, heparin and lanthanides. It demonstrated that the pH has strong effect on the interaction of endostatin and heparin.

研究了内皮抑素与锌离子、肝素、稀土离子作用后蛋白质的光谱学性质和二级结构的变化,首次发现偏酸性pH对肝素与内皮抑素的结合作用有特殊影响,为研究内皮抑素特异抑制肿瘤组织新生血管的生成提供了实验依据。

The recovery of blood circulation of ischemia semidarkness region, improvement of partly hypoxia state and prevention of more serious of impaired are key point in the stroke treatment. This experiment is designed to observe the blood vessel regeneration of ischemia injured region, and observe the variance of partly PCNA and VEGF expression. Combine with limbs functional recovery, we validate the therapeutical effect of acupuncture and investigate the possible mechanism of action.

尽早恢复缺血半暗区的血液循环,改善局部缺氧状态,防止损伤的进一步加重是脑卒中治疗过程中的重点,本实验从缺血损伤区血管再生为切入点,观察局部PCNA、VEGF表达的变化,结合肢体功能恢复情况,验证针刺的治疗作用,并探讨可能的作用机制。

The major results obtained are: PCR-RFLP method is suitable for molecular identification of Shihu; 205 compounds were isolated from 10 species of Dendrobium, including 21 new compounds, and 28 compounds isolated from genus Dendrobium for the first time. Each of the extracts and compounds were screened for anticoagulant and antitumour activities separately. The results indicated that the ethyl acetate extract from Dendrobium chrysanthun has significant anticoagulant function; the antitumour function of the 4 bibenzyl components from Dendrobium chrysotoxum is stronger than that of phenanthrene, of which the erianin has significant anti-angiogenic effect both in vivo and in vitro; components with moderate or stronger antitumour activities were found in the extracts of D. thyrsiflorum, D. chrysanthun and D. trigonopus; quality standard for 5 species of Dendrobium was drawn up use active or indicative components as control.

重要研究结果有:经方法学研究,认为PCR-RFLP法适于作为石斛属药材分子标记鉴定方法;对10种石斛进行化学成分分离鉴定,共分得205个化合物,其中新化合物21个,首次从石斛属分得的28个;对提取物和各单体化合物分别作抗凝血、抗肿瘤等活性筛选,结果说明束花石斛乙酸乙酯部位有显著的抗凝血作用,鼓槌石斛的4个联苄类成分抗肿瘤作用强于菲类,联苄类毛兰素在体内外有显著抗血管生成活性,球花石斛、束花石斛及翅梗石斛均筛到中等或较强抗癌活性的成分;选择有活性或指标性成分作对照品,对5种石斛草拟了质量标准。

Bilirubinic decomposition product has more powerful antioxidation than vitamin E;dissociative ferrum also plays roles on harmful product such as hydroxy radical and superoxide anion etc;As an important signal molecules,CO play significant roles on neuron information transfer and vascular tone regulation;people pay attention to HO,which is the main enzymatic system producting endogenous CO increasingly.

其中胆绿素的分解产物具有较维生素E更强的抗氧化作用;Fe~(2+)参与催化生成有害的活性氧代谢物,如羟自由基,超氧阴离子等;CO作为一种气体信使分子,在神经元信息传递和血管紧张度调节中起重要作用,HO作为产生内源性CO的主要酶系统日益受到关注。

The hypercholesterolemic rats were selected to imitate early stage of human atherosclerosis The effects and mechanism of n-3 PUFA on endothelium were investigated with the following parameters: the morphological appearance and arrangement of endothelium stained by Ag, in vitro endothelium-dependentrelaxation of aortic ring, endothelial permeability test using Even′s Blue, blood lipid and body weight in one-week intervals, excitative and emissive spectrum of RBC membrane labeled by DPH (1, 6-Diphenyl-1, 3, 5-hexatriene), fluidity of RBC membrane, plasma and RBC membrane LPO

在整体和离体水平,选用大鼠高脂血症模型模拟动脉粥样硬化早期状况。用114只大鼠多指标着重研究n-3多不饱和脂肪酸在高脂血症状态下对内皮的保护作用,以探讨其预防动脉粥样硬化的机理。所采用指标包括:浸银法观察内皮细胞排列与形态;离体血管环观察内皮依赖性舒张;伊文氏蓝染料法观察内皮通透性。为探讨作用机理,进行了血脂和体重的动态监测、DPH(1,6-二苯基-1,3,5,乙三烯)标记红细胞膜的荧光光谱测定、红细胞膜流动性测定、血浆和红细胞膜脂质过氧化物测定。

The digestion process includes the following steps: adding 0.15 % concentration collagenase II solution compounded with DMEM into blood vessel smooth muscle tissue block and acting in water bath for 1.5 hr until forming floccular tissue; cleaning with 0.02 % EDTA solution, adding 0.125 % trypsase solution and water bath vibrating to digest at 37 deg.c to obtain dispersed smooth muscle cells; terminating enzyme action with 10 % new born ox serum culture liquid; regulating cell concentration with 20 % new born ox serum culture liquid to 50,000-500,000/L, inoculation, and culturing in culture tank with 5 % CO2 and first 37 deg.c to cell fusion of 70-80 %; and subculturing conventionally.

向血管平滑肌组织块中,加入用DMEM配制的0.15%胶原酶II溶液,水浴箱中作用1.5h,至组织呈絮状,用0.02%的EDTA清洗,然后,加入0.125%胰蛋白酶溶液,于37℃水浴振荡消化,即可得到分散的平滑肌细胞,用10%新生牛血清的培养液终止酶的作用。加入含20%新生牛血清的培养液,调节细胞密度为5×104~5×105个/L,接种,放入5%CO2、37℃培养箱中培养。当细胞达到70%~80%融合时即可按常规方法传代。

These growth factors have the effects of neurogenesis, angiogenesis, neurotrophy, inhibitory excitoxity induced by glutamate and NMDA, and anti-oxidative, by these ways they can prevent neural cells from death.

这些生长因数均有保护神经细胞避免因为脑损伤而造成的死亡,其作用有神经生长性、血管生长性、神经滋养性、抑制麸氨酸盐与NMDA所引发的兴奋性毒性及抗氧化作用,但是这些仅限於动物模式,应用於临床还有一段艰辛的历程。

Cardiac hypertrophy and extracellular matrix remodeling happened as a result of chronic overrange of pressure in heart and hypervolaemia which lead to damage of relaxation and compliance of heart.Drugs including renin-angiotensin converting enzyme inhibitor,angiotensin receptor blocker,aderergic receptor blocker,aldrosteron antagonism,tatins endothelin receptor blocker pancreatic kiniogenase and calcalium channel blocker,can delay or reverse the remodeling by several mechanisms.

主要表现为心脏在慢性超负荷的压力、容量作用下发生的病理改变致使左室肥厚组织胶原结构发生改变,导致心肌顺应性降低,舒张功能受到损害;作用于肾素-血管紧张素系统的药物及肾上腺素受体阻滞剂、醛固酮拮抗剂、他汀类药物、内皮素受体拮抗剂、胰激肽原酶、钙通道阻滞剂等药物通过不同途径和机制延缓和逆转左心室重构。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。