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血清阴性

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Results: A total of 250 serums of measles uncertain patients were detected.

结果:250份血清样本中阳性122份,阴性128份,阳性率为48.8%。

Results 162 blood serums were all negative, accordant with the control.

结果 162份皮毛加工人群的血清样本检验均为阴性,与对照的63份样本相一致。

Quote pregnancy test first morning urine, such as positive, on the note you are pregnant, and if negative, can go to the hospital for blood serum human chorionic promote gland hormone tests, such as positive, you can clear diagnosis.

引用早孕测清晨第一次小便,如为阳性,就说明您怀孕了,如为阴性,可去医院抽血作血清人绒毛膜促腺激素化验,如为阳性,即可明确诊断。

In vivo treated sera from ATG-treated patients produced positive test results for T CDC and T/B FXM, while the B-cell CDC crossmatch remained negative.

来自于ATG治疗的病人血清测试发现,引起T细胞的补体依赖细胞毒性反应和 T/B的流式细胞计数阳性结果相关,而同时,B细胞补体依赖细胞毒性交叉配血保持阴性。

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样,利用PCR和SOE技术,获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因,以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗:二基因融合(pCDNA3.1-MPB64-Ag85B,简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6,简称pCMAE)DNA疫苗;二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗,免疫BALB/c小鼠,以牛分枝杆菌BCG免疫组为阳性对照,以pCDNA3.1及PBS免疫组为阴性对照,共免疫3次,每次间隔2周,BCG组仅初免时皮下免疫1次。1免后每周,以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原,以间接ELISA方法检测血清IgG水平及lgG亚类。

Results The body weight, visceral fat and body weight ration, as well as the levels of blood glucose, insulin, triglyceride, total cholesterol, LDL, and total FFA, were significantly lower in high fat-fed group than in control group (P.01). The aortic tunica intima was intact, the number of foam cells was decreased, and inflammatory cells infiltrated into the med-intima smooth muscle of the n-3 PUFA group. However, with no such changes were observed in control and n-3 PUFAs groups.

结果 n-3脂肪酸组大鼠体重第1~11周均明显降低,空腹血糖、血清胰岛素、TG、TC、LDL-C、总FFA和内脏脂肪占体重百分比均明显低于HF组,HDL-C则明显高于HF组,与对照组无明显差别;n-3脂肪酸组大鼠主动脉主动脉内膜完整无内皮细胞脱落现象,内膜下泡沫细胞及炎细胞浸润减少,内膜的NF-κB呈弱阳性表达,IκBα呈阳性表达,ICAM-1和MCP-1均呈阴性或弱阳性表达。

The aortic tunica intima was intact, the number of foam cells was decreased, and inflammatory cells infiltrated into the med-intima smooth muscle of the n-3 PUFA group. However, with no such changes were observed in control and n-3 PUFAs groups. Little or no expression of NF-κB, ICAM-1 and MCP-1, and a high expression level of IκBα were detected in aortic tunica intima of the n-3 PUFA and control groups, while high expression levels of NF-κB, ICAM-1 and MCP-1, and little expression of IκBα were detected in aortic tunica intima of the high fat-fed group.

结果 n-3脂肪酸组大鼠体重第1~11周均明显降低,空腹血糖、血清胰岛素、TG、TC、LDL-C、总FFA和内脏脂肪占体重百分比均明显低于HF组,HDL-C则明显高于HF组,与对照组无明显差别;n-3脂肪酸组大鼠主动脉主动脉内膜完整无内皮细胞脱落现象,内膜下泡沫细胞及炎细胞浸润减少,内膜的NF-κB呈弱阳性表达,IκBα呈阳性表达,ICAM-1和MCP-1均呈阴性或弱阳性表达。

Sera from 18 Chinese haemophiliacs treated with factor Ⅷ produced in USA from 1982 to 1984 were tested for antibody to LAV/ H T L V-Ⅲ by ELISA. Four patients ( 22.2%) showed strong positive results which were confirmed by immunofluorescence test and Western blot,but none of them has developed symptoms related to AIDS.

对浙江省1982~1984年注射了美国产浓缩Ⅶ因子制剂的18例血友病患者,用酶联免疫吸附法检测了血清中淋巴腺病病毒/人T细胞Ⅲ型病毒抗体,发现4例阳性,并经免疫荧光试验和Western印迹法证实。2例应用了国产浓缩Ⅷ因子者抗体阴性。

METHODS: 39 full-term placentae of HBsAg sero-positive and 4 full-term placentae of HBsAg sero-positive pregnant women were enrolled.

收集血清学HBsAg阳性孕妇足月胎盘组织39例,阴性4例,用SABC免疫组化法检测HBsAg、FcγRIII。

Taken the tuberculin skin test as the reference test,560 serum samples from four local cow herds in the Middle of China were assayed with iELISA.

以结核菌素皮内试验为参考方法,检测了华中地区四个奶牛场的560头中国荷斯坦奶牛和90份进口奶牛结核阴性血清,结果iELISA与TST的总符合率为87.32%(489/560)。

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