血清阴性

The negative rate of DIGFA in healthy people was 100%(40/40). The cross reaction rate in 20 cysticercosis cases and 25 schistosomiasis cases were 5%(1/20) and 4%(1/25), respectively.

DIGFA分别检测正常人血清 40例、囊虫病患者血清 2 0例、日本血吸虫病患者血清 2 5例，前者均为阴性，后两者的交叉反应率分别为 5 %和 4%。

YMDD mutation: HBV YMDD mutation were developed in 12 lamivudine recipients, whereas the treated group had no incidence of HBV YMDD variation, there were significant difference between two groups (p=0.000). YMDD mutation resulted in increase of the mean HBVDNA levels (p=0.000), but no contribution to serum ALT diversification (p＞0.05). 2. Precore nt1896 mutation: Precore nt1896 mutation incidence had no difference between lamivudine recipients and objects who received combination therapy of Kidney-invigorating Granule and Marine Injection, and patients of HBeAg negative and HBeAg positive had a close incidence of precore mutation as well as. Precore nt1896 mutation had no influence on serum ALT level. The occurrence of precore nt1896 mutation contributed to serum HBVDNA load increased and worse response of HBVDNA extenuation. 3. HBV genotype: The baseline of serum HBVDNA gradually increase in turn of genotype B, genotype C and genotype B/C. Genotype B seemed to obtain higher rate of serum HBVDNA loss and HBeAg/anti-HBe seroconversion after combination therapy of Kidney-invigorating Granule and Marine Injection, differencing with lamivudine therapy.

疗效相关因素研究：1YMDD变异：对照组有12例检出YMDD变异，治疗组无1例检出，两组比较有显著性差异（P=0.000）；发生YMDD变异者血清HBVDNA和ALT水平均有所升高，HBVDNA平均水平高于非变异者（P=0.000）；血清ALT与非变异者无明显差异（P＞0.05）。2前C区nt1896变异：前C区nt1896变异发生率在治疗组和对照组以及HBeAg阴性和HBeAg阳性患者之间均无明显差异（P＞0.05）；1896c变异对血清ALT水平无明显影响；治疗组发生1896c变异者，其HBVDNA平均水平较之未变异者明显升高（P＜0.05），治疗后HBVDNA下降幅度明显低于未发生1896c变异者（P=0.000）。3HBV基因型：血清HBVDNA基础水平在B、C、B/C混合型间存在明显差异（P＜0.05），按B型、C型、B/C混合型依次升高；治疗组HBVDNA阴转率、HBeAg/抗-HBe血清转换率，B型似乎高于C型和B/C混合型，但无统计学意义（P＞0.05）；对照组HBVDNA阴转、HBeAg/抗-HBe改变在B、C、B/C混合型间无明显差异（P＞0.05）。

Methods: Applying TP-ELISA, TRUST and colloidal gold test to test 102 positive sera, 220 negative sera and 5 indeterminable sera were tested by TPPA, respectively. Then we compared the sensitivity and specificity of the three methods.

分别用TP-ELISA、TRUST、金标法对102份TPPA确证阳性血清，5份TPPA可疑血清，220份TPPA确证阴性血清进行实验，比较3种梅毒初筛方法的敏感度和特异度。

Methods Sera derived from 226 SLE cases and 183 healthy controls were tested for ANA and all parameters were compared such as sensi- tivity,specificity,accuracy,positive predictive value,negative predictive value,positive likelihood ratio,nega- tive likelihood ratio,result consistency,rank correlation coefficient and kappa of ANA detected by IFA...

方法同时采用IFA法和ELISA法检测血清ANA共409例，其中SLE 226例和健康对照183名，将两种方法检测结果的敏感度、特异度、准确度、阳性预测值、阴性预测值、阳性似然比、阴性似然比、结果符合率、判断一致性κ值及秩相关系数进行比较分析；通过ROC曲线比较两种方法的准确度；结果不一致标本进行可提取性核抗原抗体谱检测分析；同时将IFA法检测不同荧光模型样本分别与ELISA结果进行相关分析。

Circulating antigen was detected in 29 out of 70 cases with paragonimiasis with a sensitivity of 41.5%. The rate of cross reaction in cases with clonorchiasis sinensis and schistosomiasis was 25%（5/20） and 20%（4/20）, respectively, and it was negative in 60 casess with other parasitic infections and healthy subjects, with an overall specificity of 93.6%.

用CAg-dot-ELISA检测70份卫氏并殖吸虫病临床诊断患者血清，阳性29份，敏感性为41.5%（29/70），与华支睾吸虫病和日本血吸虫病患者血清分别有25%（5/20）和20%（4/20）的交叉反应，与其他寄生虫感染者血清和健康人血清（60份）均为阴性，特异性为93.6%。

The results showed that the 56kD and 26kD antigens had better immunogenicity and could not react with the sheep positive serum of Mycoplasma pneumonia and negative serum of E. ovis. Of the 64 samples detected, the positive rate was 66.7%(42/64), 22.9% higher than that of direct microscopic examinerion.

结果表明，56kDa和26kDa的羊附红细胞体抗原具有较好的免疫性，而且不与羊附红细胞体阴性血清和羊支原体病阳性血清发生交叉反应，具有较好的特异性；ELISA检测的64份血清样本的阳性率为66.7%，比涂片镜检高22.9个百分点。

Results:Serum CYFRA21-1 level was significantly higher in the patients with protopathic breast cancer before operation(the positive rate was 21%)than the patients with benign breast diseases (serum CYFRA21-1 were all negative).

结果：乳腺良性疾病无1例阳性；乳腺癌术前阳性率仅21%，但两者血清 CYFRA21-1 水平有明显差异；47例乳腺癌患者手术前与术后 4周血清 CYFRA21-1 水平相比有显著差异；56例乳腺癌患者术后随访 8例转移复发者中 6例阳性，无转移复发者均阴性，转移复发者血清 CYFRA21-1 水平较无转移复发者明显增高，两者相比差异有极显著性。

Results:Serum CYFRA21-1 level was significantly higher in the patients with protopathic breast cancer before operation(the positive rate was 21%)than the patients with benign breast diseases (serum CYFRA21-1 were all negative).

结果ˇ乳ˇ良性疾病无1例阳性ˇ乳ˇ癌术前阳性率仅21%ˇ但两者血清 CYFRA21-1 水平有明ˇ差异ˇ47例乳ˇ癌患者手术前与术后 4周血清 CYFRA21-1 水平ˇ比有ˇ著差异ˇ56例乳ˇ癌患者术后随访 8例转移复发者中 6例阳性ˇ无转移复发者均阴性ˇ转移复发者血清 CYFRA21-1 水平较无转移复发者明ˇ增高ˇ两者ˇ比差异有极ˇ著性。

Anti-MOG and anti-MBP antibodies predicted more frequent and earlier relapses. Of 39 seronegative patients, nine (23%) had a relapse, and time to relapse was 45 13.7 months. Of 22 patients with anti-MOG and anti-MBP, 21 (95%) relapsed, and mean time to relapse was 7.5 4.4 months. Of 42 patients with only anti-MOG, 35 (83%) relapsed within 14.6 9.6 months P .001 for both comparisons with antibody-seronegative patients.

研究显示，抗MOG和抗MBP抗体可以预测更多和更早期的发作，39名血清呈阴性的患者中，9位(23%)复发，而重新发作的时间是45 ± 3.7个月，22名带有抗MOG和抗MBP的患者中，21名(95%)复发，复发时间为 7.5 ± 4.4个月，另外42名患者只有抗MOG，35人(83%)在 14.6 ± 9.6个月之内复发（P 。001，二组相较於血清呈阴性的患者）。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。