血清阴性

By using the above established ELISA,we Examined 35 cases of HSV-1 infected serum and 43 cases of negative serum.The results showed 91.4%of sensitivity,97.6%of specificity,97.0%of positive predictive value,93.3%negative predictive value,accuracy rating 94.5%.

以上述条件建立的ELISA对35份HSV-1感染的阳性血清和43份阴性血清进行检测，其敏感度为91.4%；特异度为97.6%；阳性预测值为96.9%；阴性预测值为93.3%；准确度为94.8%。

Kala-azarpatients were examined by ELISA with three Leishmania strains antigen, the results were 901 strain with 80.92%, 801 strain with 92.25% and 951 strain with 89.92%. The result was notable difference between 901 strain and 801 strain. 334 healthy persons serum of endemic areas were tested by ELISA with three Leishmania strains antigen, the positive rates were notable differenceamong three antigens.

结果：提取的三株抗原的蛋白浓度分别为：901株1000ug/ml、801株800ug/ml、951株1000ug/ml；工作浓度均为10ug/ml；三株抗原分别检测24份肺结核病人血清，均为阴性；三株抗原分别检测60份非疫区健康人血清，801株与951株的检测结果均为阴性，901株检测出一份阳性；三株抗原分别检测131份黑热病病人血清，阳性率分别为：901株80.92%、801株92.25%、951株89.92%。801株明显高于901株。

Results The testing results showed that this method with 100% specificity and sensitivity in 300 positive and 100 negative samples of mammalian sera respectively. However, the positive serum samples of Francisella tularensis, yersinia pseudotuberculosis and yersinia enterocolitis could not be deceted by dipstick of ICA.

结果 间接血凝法检测阳性300份血清标本，胶体金法检测全部为阳性，间接血凝法检测阴性100份血清标本，胶体金法检测全部为阴性，两种方法符合率100％；并且鼠疫胶体金法快速诊断盒不与假结核耶尔森氏菌等相关细菌的免疫血清发生交叉反应。

ELISA method was used for comparison. Results The positive rate of DIGFA in testing the serum from 50 infected rats was 96.0%(48/50). The negative rate of DIGFA in healthy rats was 100%(50/50). Cross reaction with Enteroclysis (13 samples) and Tapeworm (11 sqamples) was not observed. The cross reaction rate in 18 Strongyloidiasis rats was 5.6%. The sensitivity and specificity of DIGFA is comparable with ELISA (96.7%).

结果 用DIGFA检测广州管圆线虫实验感染鼠血清50份，其阳性检出率为96.0%，50份正常鼠血清的阴性符合率达100%，DIGFA分别检测蛲虫阳性鼠血清13份，绦虫阳性鼠血清11份和粪类圆线虫阳性鼠血清18份，前二者均为阴性，后者交叉反应率为5.6%；DIGFA与ELISA平行检测30份广州管圆线虫阳性鼠血清，两法符合率达96.7%。

The results showed that in evaluation of the method by detecting 50 RHD 1227A positive and 50 RHD 1227A negative individuals, the genotyping method displayed a sensitivity of 100% and a specificity of 100%; in evaluation of the method by detecting 33 DEL positive and 89 DEL negative individuals, the sensitivity was 100%, however, there were two serologically negative samples which were confirmed as positive using genotyping method.

结果表明：在50例RHD 1227A阳性和50例RHD 1227A阴性的Rh阴性样本中基因分型方法的灵敏度和特异性都是100％；在33例DEL阳性样本和89例DEL阴性的样本中，基因分型方法的灵敏度为100％，有2例样本血清学结果为阴性而基因分型结果为阳性，重新用血清学方法和序列分析方法复核这2例样本，发现2例都是血清学漏检，因而基因分型方法的特异性是100％。

The results showed that in evaluation of the method by detecting 50 RHD 1227A positive and 50 RHD 1227A negative individuals, the genotyping method displayed a sensitivity of 100% and a specificity of 100%; in evaluation of the method by detecting 33 DEL positive and 89 DEL negative individuals, the sensitivity was 100%, however, there were two serologically negative samples which were confirmed as positive using genotyping method. After re-testing these two samples with serological method and sequence analysis, it was found that original serological method gave false negative results and genotyping method still showed 100% specificity. The minimal target DNA concentration of this genotyping method is 8.13 ng/μl.

结果表明：在50例RHD 1227A阳性和50例RHD 1227A阴性的Rh阴性样本中基因分型方法的灵敏度和特异性都是100％；在33例DEL阳性样本和89例DEL阴性的样本中，基因分型方法的灵敏度为100％，有2例样本血清学结果为阴性而基因分型结果为阳性，重新用血清学方法和序列分析方法复核这2例样本，发现2例都是血清学漏检，因而基因分型方法的特异性是100％。

Results The level of AAG,AAT,HPT and CER in patients with RA and seronegative spondyloarthropathy and all kinds of arthritis was significantly higher than that in normal controls (P＜0.001) and was positively correlated with that of ESR and CRP.

结果 RA、血清阴性脊柱关节病及关节炎综合组患者该4项指标浓度均显著高于正常对照组(P＜0.001)。并与血沉、C-反应蛋白呈正相关。

Anti-MOG and anti-MBP antibodies predicted more frequent and earlier relapses. Of 39 seronegative patients, nine (23%) had a relapse, and time to relapse was 45 13.7 months. Of 22 patients with anti-MOG and anti-MBP, 21 (95%) relapsed, and mean time to relapse was 7.5 4.4 months. Of 42 patients with only anti-MOG, 35 (83%) relapsed within 14.6 9.6 months P .001 for both comparisons with antibody-seronegative patients.

研究显示，抗MOG和抗MBP抗体可以预测更多和更早期的发作，39名血清呈阴性的患者中，9位(23%)复发，而重新发作的时间是45 ± 3.7个月，22名带有抗MOG和抗MBP的患者中，21名(95%)复发，复发时间为 7.5 ± 4.4个月，另外42名患者只有抗MOG，35人(83%)在 14.6 ± 9.6个月之内复发（P 。001，二组相较于血清呈阴性的患者）。

Results The sensitivity,specificity,positive predictive value,negative predictive value,Youden index,accuracy ...

结果检测血清特异性IgG的灵敏度为100·0%，特异度为97·7%，阳性预测值为97·2%，阴性预测值为100·0%，约登指数为0·977，符合率为98·7%，试验的一致率为98·5%；检测血清中总的特异性Ig灵敏度为97·1%，特异度为95·3%，阳性预测值为94·4%，阴性预测值为97·6%，约登指数为0·925，符合率为96·2%，试验的一致率为97·8%；检测唾液中特异性sIgA的灵敏度为96·2%，特异度为94·5%，阳性预测值为89·3%，阴性预测值为98·1%，约登指数为0·907，符合率为95·1%，试验的一致率为97·5%；检测粪便标本中的特异性sIgA的灵敏度为92·0%，特异度为90·2%，阳性预测值为85·2%，阴性预测值为94·9%，约登指数为0·822，符合率为90·9%，试验的一致率为98·6%，CV值均小于15%。

Objective To understand the distribution and immune activation status of T lymphocyte subsets in highly exposed HIV1 and persistently seronegative individuals, and explore the immunologic mechanism of nonsusceptibility to HIV1 by sexual transmission in HEPS individuals.

目的 了解经性传播途径高暴露HIV1持续血清阴性者淋巴细胞亚群表达及其免疫活化状态，探讨经性途径高暴露HIV1不易感的免疫基础。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。