血清酶

Serum creatine kinase and isodynamic enzyme, lactate dehydrogenase and isodynamic enzymel (LDH1),α-hydroxybutyrate dehydrogenase in 3 group were detected with electrocardiography and clinical analysis was made.

分别检测各组血清肌酸激酶及其同工酶，乳酸脱氢酶及其同工酶1(LDH1)，α-羟丁酸脱氢酶水平，同时行心电图检查。

To investigate the ability of ADA isozyme expression to discriminatebetweem lymphoid and nonlymphoid cell types in acute leukemia,the gelfiltration with FPLC system was used as micromethod for ADA isozymeanalysis.

为进一步阐明白血病细胞ADA同工酶生化性质，探讨制备ADA同工酶抗体的可能性，我们采用分子筛FPLC和阴离子交换FPLC系统二步法分离纯化健康人血清、单个核细胞和急性粒单核细胞白血病细胞ADA同工酶，获得部分纯化ADA1和ADA2，ADA1纯化71.62倍，活力62.2u/mg pr，ADA2纯化37倍，活力42.8u/mg pr，ADA总回收率44%，2步FPLC层析法酶活力回收率分别为68.4%和81.5%。

After feeding of 20 d, 40 d and 60 d, the activities of nitric oxide synthase, bacteriolytic, superoxide dismutase, alkaline phosphatase and acid phosphatase in the serum were determined and the relativities between them were also analyzed.

在饲料中添加不同剂量的嗜酸小球菌投喂凡纳滨对虾，并在投喂后的20、40和60d，分别测定凡纳滨对虾血清中的一氧化氮合酶、溶菌活力、总超氧化物歧化酶、酸性磷酸酶、碱性磷酸酶，并分析了这5种酶之间的相关性。

Methods Myocardial ischemia was induced by subcutaneous injection of ISO (30 mg/kg for two days, once a day). The experimental animals were randomly divided into the normal group, ISO group, positive control group and three crocetin groups (25, 50, 100 mg/kg). Cardiac indexes were examined. The level of LDH, CK, MDA in serum were measured. The content of MDA, GSH-Px and the ATPase activity heart and mitochondria were assayed by colorimetric analysis. The histopathological change of myocardia was investigated by HE staining.

采用大剂量ISOsc诱导产生大鼠急性心肌缺血模型(1SO30mg／kg，每日一次，连续2d)，实验大鼠随机分为正常组、ISO模型组、阳性对照组和3个西红花酸给药组(25，50，100mg／kg)，测定心脏系数和血清中乳酸脱氢酶、磷酸肌酸激酶和丙二醛含量，观察心肌组织谷肮甘肤过氧化物酶、Na^ ，K^ —ATP酶和Ca^2 ，Mg^2 —ATP酶活性及心脏病理改变，观察西红花酸的保护作用。

The antisera against Chaetoceros debilis, Heterosigma akashiwo, Prorocentrum micans and Gymnodinium sanguineum were used to establish the icELISA respectively.

本研究选取特异性较好、具有高效价的抗柔弱角毛藻抗血清、抗赤潮异弯藻抗血清、抗海洋原甲藻抗血清和抗红色裸甲藻抗血清，分别建立了相应海藻的竞争型酶联免疫检测方法。

Serially observed the serum active enzymatic of 5′-NT changing in process of hepatic diseases might be a biological prognostic marker, together with ALT,γ-GGT and ALP, it can be used to distinguish and diagnose most hepatic and osteal diseases.

2联合检测血清5′-核苷酸酶(5′-NT)，谷丙转氨酶和γ-谷氨酰转肽酶，碱性磷酸酶酶活性的检测有助于鉴别诊断肝脏疾病、胆道疾病和骨骼疾病。

objective:to investigate the influence of soybean isoflavones on the antioxidative effect and improving the ability of study and memory in climacteric rats.methods:forty-eight female sd climacteric rats were randomly divided into 4 groups with one control group and the other low,medium and high dose groups,which were given soybean isoflavones 0 mg/kg.bw,42 mg/kg.bw,83 mg/kg.bw and 249 mg/kg.bw by superalimentation daily for 60 days respectively.the levels of sod,gsh-px and mda in serum were measured,and the ability of study and memory in each group was deterimined by adopting the law of diving platform.results:the content of serum mda in medium and high dose groups was lower than that in control group.the activity of serum gsh-px im medium and high dose groups was significantly increased,so was the activity of serum sod in low,medium and high dose groups.conclusion:soybean isoflavones do have the obvious effect of antioxidation,and improve the ability of study and memory effectively in female sd climacteric rats.

目的：观察大豆异黄酮对更年期雌性sd大鼠抗氧化作用及学习记忆的影响。方法：选用健康更年期雌性sd大鼠，随机分成空白对照组和低、中、高剂量4组。空白对照组给予蒸馏水，实验组每日分别灌服42、83、249 mg/kg剂量的大豆异黄酮，连续60d。第60d取血测定血清过氧化脂质丙二醛含量、超氧化物歧化酶活力、谷胱甘肽过氧化物酶活力，用跳台实验方法测定大鼠学习记忆能力。结果：与空白对照组比较，中、高剂量组大鼠血清mda含量显著降低，低、中、高剂量组血清sod活力明显升高，中、高剂量组血清gsh-px活力明显升高。结论：大豆异黄酮具有明显的抗氧化作用，并有效提高更年期雌性sd大鼠的学习记忆能力。

Six and twelve boors after sepsis, leptin levels in liver homogenate were detected by radioimmunoassay serum alanine aminotransferase and 4 enzymes in liver homogenate. myeloperoxidase, glutathin-S-transferase, xanthine oxidase and superoxide dismutase, all related with synthesis of free radicals, detoxication and purine metabolism, were detected by 96 well spectrophotometry. H-E staining was used to examine the histopathologic changes of liver.

于脓毒症后6 h和12 h，采用放射免疫法测量肝组织匀浆液中Leptin水平，采用96孔分光光度法检测血清丙氨酸氨基转移酶及髓过氧化物酶、谷胱甘肽－S转移酶、黄嘌呤氧化酶、超氧化物歧化酶等4种与自由基合成、解毒和嘌呤生成代谢相关的酶的水平，同时以H-E染色法观察肝组织病理学改变。

At the endpoint, the thymus, spleen and uterus were weighed separately; the femoral dry weight, bone-calcium, phosphor, magnesium and hydroxyproline contents of femoral were measured; the histomorphological features of bone were observed and the biochemical indexes in serum (Ca, P, ALP, TRAP, E2, BGP) were determined after the mice were given SAR at the dose of 10, 30, 90 mgkg^(-1) respectively, and were compared with the model group, the normal control and the positive control. RESULTS: The osteoporosis of high turnover type was formed with feeding retinoic acid to experimental mice. Compared with the model group, the lessening of femoral dry weight, the short femoral transverse diameter, the decrease of bone mineral and bone-hydroxyproline of SAR groups were improved.

维A酸105mgkg^(-1致小鼠骨质疏松模型，SAR10、30、90mgkg^(-1灌胃给药，实验过程监测体重，2周后以比色法测定血清钙、磷含量和碱性磷酸酶、抗酒石酸酸性磷酸酶活性，竞争放射免疫法测定血清中骨钙素、雌二醇(s-E2)含量，处死动物后取脾、胸腺、子宫称重，取股骨进行骨组织形态学观察，测定并比较骨计量学和骨钙、骨磷、骨镁及骨弃脯氨酸含量。

Litopenaeus vannamei were continuously fed by basic diets containing three levels (1%, 0.5%, 0.1%) Ig-Guard respectively for 20 days, and the basic diets as blank control. Phenoloxidase, acid phosphatase, alkaline phosphatase, lysozyme activity of the serum and superoxide dismutase, catalase activity of the muscle, and protein concentration of the serum and the muscle were detected in the 5, 10, 15, 20 day.

采用连续投喂的方法，饲喂凡纳滨对虾 20 d，分别测定了第5，10，15，20 d血淋巴的酚氧化酶、溶菌酶、酸性磷酸酶、碱性磷酸酶及肌肉匀浆液的超氧化物歧化酶、过氧化氢酶等非特异性免疫因子活性，并对血清及肌肉匀浆液中蛋白进行定量。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。