血清酶

A method for determination of Secoisolariciresonol diglucoside, secoisolariciresinol, enterolactone and enterodiol in serum by LC/MS/MS was established. In this method,β-Glucuronidase was used to hydrolyze the serum samples, the electrospray ionization sourse and negative ion MRM scan were employed.

本实验用β葡萄糖醛酸苷酶酶解血清样品，采用电喷雾离子源，负离子MRM扫描，建立了血清中司可异罗叶松甘油二酯、司可异罗叶松酯素、肠内二醇、肠内脂的IC/MS/MS分析方法。

The digestion process includes the following steps: adding 0.15 % concentration collagenase II solution compounded with DMEM into blood vessel smooth muscle tissue block and acting in water bath for 1.5 hr until forming floccular tissue; cleaning with 0.02 % EDTA solution, adding 0.125 % trypsase solution and water bath vibrating to digest at 37 deg.c to obtain dispersed smooth muscle cells; terminating enzyme action with 10 % new born ox serum culture liquid; regulating cell concentration with 20 % new born ox serum culture liquid to 50,000-500,000/L, inoculation, and culturing in culture tank with 5 % CO2 and first 37 deg.c to cell fusion of 70-80 %; and subculturing conventionally.

向血管平滑肌组织块中，加入用DMEM配制的0.15％胶原酶II溶液，水浴箱中作用1.5h，至组织呈絮状，用0.02％的EDTA清洗，然后，加入0.125％胰蛋白酶溶液，于37℃水浴振荡消化，即可得到分散的平滑肌细胞，用10％新生牛血清的培养液终止酶的作用。加入含20％新生牛血清的培养液，调节细胞密度为5×104～5×105个/L，接种，放入5％CO2、37℃培养箱中培养。当细胞达到70％～80％融合时即可按常规方法传代。

Objective To evaluate diagnostic value of anti-deoxyribonuclease B microtitration test in rheumatic fever. Methods Anti-DNase B antibodies of 87 patients with acute rheumatic fever and 202 rheumatic heart disease patients with rheumatic activity were detected with anti-DNase B antibody microtitration test,and compared with antistreptolysin "O" test.

目的 评估A组溶血性链球菌感染的血清学测定方法对诊断风湿热的临床价值方法采用抗DNA酶B微量法和自制试剂测定急性风湿热87例、活动期风湿性心脏病202例病人血清的抗DNA酶B抗体，与抗溶血素O抗体对照研究。

Results:There were no abdominal pain and fever,jaundice faded away gradually postoperation.Biliary drainage was 300ml-1000ml/d more or less.Colour of bile changed into normal by degrees.Intra peritoneal drainage was open and the colour was light bloody.Amount of drainage dropped off day after day.Whole blood cell test, serum glutamic pyruvic transaminase,serum glutamic oxalacetic transarninase,total serum protein,serum albumin and serum globulin returned to normal in first week postoperation.Serum bilirubin,serum glutamyltranspetidase and serum alkaline phosphatase declined gradually in first week postoperation.T-tube was clipped at twelfth day postoperation.T-tube Cholangiography was clear at 24~ day postoperation.

结果：术后无上腹部绞痛、高热，黄疸逐渐减退，每日引流胆汁300ml-1000ml不等，由淡黄色混浊、内有胆泥过渡到金黄色清亮的正常胆汁，腹腔引流通畅，引流液为淡红色，量从最初的200ml迅速过渡到10ml，血常规、血清谷丙转氨酶、谷草转氨酶、总蛋白、白蛋白、球蛋白等1周内均恢复正常，血清总胆红素、直接胆红素、间接胆红素、谷氨酰转肽酶、碱性磷酸酶逐渐下降，术后12d夹闭T管，术后24d T管造影提示左、右肝管及胆总管下端通畅，无狭窄。

Objective: To investigate the relationship among cholinesterase and Child -Pugh score of cirrhosis. To investigate the clinical significance of serum cholinesterase of patients with liver cirrhosis.

目的：探讨肝硬化患者Child-Pugh分级与血清胆碱酯酶(cholinesterase，ChE)活性的相互关系，以及肝硬化患者血清胆碱酯酶活性测定的临床意义。

However, there were no significantly differences on serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase between these four groups.

然而在肝功能方面，不论是血清麸草酸转胺基酶或血清麸丙酮酸转胺基酶，四组都没有显著性的差异。

Results Patients who had at least one abnormal result of liver function test accounted for 70.99% in untreated group and 61.07% in treated group. The most common abnormal hepatic function parameter in patients with thyrotoxic hepatic lesion was increased alkaline phosphate enzyme, which was 57.3% in untreated group and 51.0% in treated group. Abnormalities of alanine transaminase, aspartate transaminase, gamma-glutamyl tyanspeptidase and total bilirubin were 28.2%(37/131), 18.3%(24/131), 10.7%(14/131) and 21.4%(28/131) in untreated group and 21.5%(32/149), 15.4%(23/148), 4.7%(7/149) and 20.1%(30/149) in treated group, respectively. The possibility of thyrotoxic hepatic dysfunction was correlated with the patients' age and FT3 and FT4 levels, but not with the genesis and course of disease, sex, family history of hyperthyroidism, or levels of TGAb and TMAb. Conclusion Abnormal results of liver function test are commom in patients with hyperthyroidism.

结果 在未治组甲亢性肝功能损害的发生率为70.99%，经治组为61.07%；甲亢性肝功能损害最常见的异常指标是碱性磷酸酶增高，未治组、经治组分别为57.3%、51.0%；血清谷丙转氨酶异常在两组的发生率分别为28.2%、21.5%；血清谷草转氨酶异常在两组的发生率分别为18.3%、15.4%；总胆红素异常在两组的发生率分别为21.4%、20.1%；谷酰胺转肽酶异常的发生率未治组和经治组分别为10.7%、4.7%；甲亢性肝功能损害的发生与病程、甲状腺疾病家族史、性别、TGAb水平、TMAb水平无关，与年龄、甲状腺激素水平有关。

Methods Three serologic methods including domestic-made enzyme-linked immunosorbent assay reagents, the toluidine red unheated-serum test and treponema pallidum particle agglutination test reagents were compared. The transudate fluid of the syphilis patient in genital duct was detected by polymerase chain reaction for analysis of infectivity.

采用国产酶联免疫吸附试验试剂与甲苯胺红不加热血清试验和梅毒螺旋体颗粒凝集试验进行血清学方法比较，采用聚合酶链反应对梅毒患者生殖道皮损渗出液进行检测，分析其传染性。

All the cases were phlebotomized venously 8ml under fasting condition in the morning.Serum No and IL-6 were determined by colorimetry and radioimmunoassay method respectively.

血清NO采用硝酸还原酶法检测，血清IL-6 采用放免法检测，血浆D-二聚体和GMP-140采用酶联免疫吸附双抗体夹心法原理定量测定。

All the cases were phlebotomized venously 8ml under fasting condition in the moming.Serum No and IL-6 were determined by colorimetry and radioimmunoassay method respectively.

血清NO采用硝酸还原酶法检测，血清IL-6采用放免法检测，血浆D-二聚体和GMP-140采用酶联免疫吸附双抗体夹心法原理定量测定。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。