血清酶

A set of spectific primers was syn thesized according to HBV DNA sequence of Chinese strain, the whole X region was amplified by PCR method from the serum of 9 patients with chronic HBV infection , and then the PCR products were subcloned into pGEM Teasy vectors. Clones were randomly selected to be sequenced. Comparison of the cloned sequence was made to find the difference. After being compared, each sequence of selected clones is o f difference. The point mutation scattered through X region. Deletion mutations were detected in 19 clones of 37(51.4%), which caused different carboxyl endings of X protein. There is a hot region (after 123 aa code) where deletion mutation frequently happens.

以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，自9例慢性HBV感染患者血清中扩增HBV X基因，克隆入pGEM Teasy质粒，随机挑选克隆进行D NA测序以确定病毒的变异程度。37例测序结果提示来源于不同患者HBV X基因序列高度保守，但每个序列均不一致。X区除了存在广泛的碱基点替换突变外，序列的缺失突变占测序克隆总数的51.4％（19／37）；氨基酸缺失及移框突变多发生于123位氨基酸残基之后，可导致X蛋白多种羧基端形式。

The concentrations of serum thyrotropin in 48 normals, 20 hatients with hyper thyroidism,10 patients with hypothyroidism and 121 inhabitants in the endemic goiter area were measured by monoclonal-antibody two-side solid-phase EIA and immunoradiometric assay.

应用单克隆抗体双面固相酶联分析法和免疫放射分析法测定48例正常人，20例甲亢病人、10例甲低病人和121例地方性甲状腺肿流行区居民的血清TSH.TSH—EIA法的结果平均值在各组中均低于IR MA法的结果。

The method was demonstrated by the separation of tryptic digestion of bovine serum albumin.

该方法以牛血清白蛋白的胰蛋白酶酶解液为样品进行分离分析。

Methods After enzymatic digestions of rat testis,the suspension passed the BSA uncontinuous gradient medium in a gravity unit by velocity sedimentation.

方法酶消化法制备大鼠睾丸组织单细胞悬液，采用牛血清白蛋白不连续密度梯度沉降法分离Sertoli细胞和精原细胞。

MethodsThrough enzymelinked immunosorbent Assay,the the serum sICAM1,sVCAM1 and sC5b9 levels were compared in 28 healthy pupils、26 asthmatic children (uninhaled glucocorticoids group) and asthmatic children after inhaled glucocorticoids 3,6 and 12 months.

方法采用酶联免疫吸附法，检测了200201—200412于青岛大学医学院附属医院儿科就诊的26例哮喘患儿吸入糖皮质激素治疗前及治疗后3个月、6个月、12个月后血清细胞间粘附因子1(sICAM1)、血管内皮细胞粘附因子1(sVCAM1)和补体激活片段(sC5b9)的质量浓度变化，并与28名健康儿童比较。

The results show that the properties of different acetylated chitosan membranes have little difference. But they all have high moisture adsorption and transparency, a certain extent permeation and adsorption of bovine serum protein, and can be degradated by lysozyme in vitro.

结果表明乙酰化壳多糖膜的性质随着乙酰化程度的不同性质会有所不同，但它们都有很好的吸水性和透光性，有比较高的结晶性，在体外可被溶菌酶降解，对于小牛血清蛋白有一定的吸附性。

Methods The asphyxiated neonates were randomly divided into two groups. Neonates in control group were cured by conventional therapy, and in adenosine group were cured by adenosine at the same time.

60例窒息新生儿随机分为治疗对照组和腺苷治疗组，均予常规治疗，腺苷治疗组同时予腺苷治疗，分别在1、7 d测定各组新生儿血清心肌酶水平。

Activity of alkaline phosphatase and ability of formation for calcifying nodules were determined.

抽取健康成人骨髓组织，用Percoll分离液分离出骨髓中的单个核细胞，在含体积分数为10%小牛血清的高糖DMEM培养液中，置于37℃、含体积分数为5%的CO2湿化空气孵箱中培养，通过传代培养扩增骨髓基质干细胞，传三代时改用含地塞米松、β-甘油磷酸和维生素C的条件培养基培养，用倒置显微镜、HE染色观察增殖和分化情况，并测定碱性磷酸酶活性和钙结节形成能力。

Methods Among the McAb against DEN1-NS1, antibody pairs were analyzed to choose the optimal coaling McAb and detecting McAb by determinating the detection sensitivity and specificity.

目的以登革病毒特异性非结构蛋白1（NS1）单克隆抗体为基础建立Ⅰ型登革病毒（DEN1）抗原检测的酶联免疫吸附法，并探索从病人早期血清样品中检测DEN1-NS1的可行性。

METHODS An experimental method was developed which allowed the isolation and culture of GER cells from P1 rat cochleae using a combinatorial approach of enzymatic digestion and mechanical separation.

取出生后第1天的大鼠耳蜗，利用机械分离和酶消化相结合的方法分离出纯的GER细胞，于含10%血清OMEM培基中进行培养。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。