血清酶

Methods Diabetic rat models were prepared by giving alloxan. After administration of sodium orthovanadate for seven days, the blood glucose levels of rats were determined. The glucose tolerances of diabetic rats were evaluated by the oxidation-enzyme method, and the insulin levels were determined by radioimmunoassay.

建立四氧嘧啶诱导的糖尿病大鼠模型，对正常大鼠及糖尿病大鼠给予原钒酸钠治疗7天后，采用葡萄糖氧化酶法测定大鼠的血糖值、糖耐量，并以放射免疫法测定血清胰岛素值。

In this study, the ovarial cancer stem cells were successfully obtained through the suspension culture of the ovarian cancer 0VCAR3 cell line in serum-free media. The stem cells formed spherical colonies, called "ovarispheres", and showed anchorage-independent ability. Furthermore, the bionomics and morphology of the stem cells were observed, and the expression difference of the tumor stem cells marker-ABCG2 between ovarispheres and anchorage-dependent cells was identified by using RT-PCR and Immunocytochemistry methods.

本研究通过无血清悬浮培养人卵巢癌细胞系OVCAR3，获得具有非锚着依赖能力的肿瘤干细胞球，观察其生物学特性及形态学的变化，并采用半定量逆转录—聚合酶链反应及细胞免疫化学的方法鉴定肿瘤干细胞标志ABCG2在贴壁细胞及肿瘤干细胞球中的表达差异。

In order to understand the mechanisms of livial in prevetion of bone loss in patients with premeture ovarin failure.12 cases pstients were treated with livial for two months.Then fasting urinary calcium creatinine ratio、 hydroxyproline creatinine natio、 Serum akaline phosphotase、 estradio(E2)、 Calcitonin、 bone gla-protein and interleukin-6(IL-6) were determined for these patients before and after treatment.

本文通过给12例卵巢早衰患者利维爱用药2个月，并于给药前后分别测定空腹尿钙与肌酐、羟脯氨酸与肌酐比值以及血清碱性磷酸酶、雌二醇(E2)、骨钙素、降钙素和白细胞介素-6(IL-6)的水平，以探讨Livial防止卵巢早衰患者骨量丢失的作用机制。

A acute partial obstructive hepatocholangitis model by selective ligation and injection of E coli into left hepatic bile duct was successfully founded in rat. Using parameters including mortality, mitochondrial glutamic oxalacetic transaminase and ornithine carbamoytransferase activity, pathological observation and blood culture of bacteria, we evaluated the model.

作者采用选择性左肝管穿刺注菌法成功地建立了Wistar大鼠急性部分肝胆管梗阻感染的脓毒症模型，并从胆管系统形态学、累计病死率、血清线粒体谷草转氨酶和鸟氨酸氨基转移酶活性、多器官大体病理观察以及血液细菌培养几方面对该模型的科学性、重复性进行了评估。

Methods Serological test and PCR method were used to detect 117 of 15 species of wild animals and 99 domesticated Paguma larvata .

对广西境内15种117只野生动物及99只人工养殖的果子狸采用聚合酶链反应和血清学方法检测其病毒核酸。

Experimental materials include CO2 incubator,M199, fetal calf serum,Ⅳ collagenase, 40 g/L of paraform, 20 g/L of cidex,ECGF, mouse-anti-human FⅧ monoclonal antibody(anti-FⅧ-RAg), multi-marked immune analyser(1 420, WALLAC BLCTOR2), centrifuge, inverted microscope, and immunofluorescence microscope, etc.METHODS: Three-step gradient sieve method was used.

实验材料为CO2培养箱，M199，胎牛血清，Ⅳ型胶原酶，40 g/L多聚甲醛，20 g/L戊二醛，ECGF，鼠抗人第八因子单克隆抗体，多标记免疫分析系统(1 420，WALLAC BLCTOR2)，离心机，倒置显微镜，免疫荧光显微镜等。

The bone mineral density and Z score in distal 1/3 radius were measured with dual energy X-ray absorptiometry in 185 he modialysis patients, meanwhile serum parathyroid hormone, alkaline phos phatase and hand X-rays were detected.

采用双能X线骨密度测定法检测185例慢性肾衰血透患者的桡骨骨密度，计算其Z值，同时检测其血清甲状旁腺素，碱性磷酸酶和指骨X照片。

The proportion of GGT complexed with low density lipoproteins plus very-low density lipoproteins in total GGT in serum is determinated with ratio colorimetery,sodium phosphotungstate and MgCl2 as precipitation.26 patients with liver cancer,18 with liver cirrhosis,22 with chronic hepatitis,14 with the other malignant tumors,16 with non-hepatobiliary disease of high GGT and 50 healthy subjects have been evaluated.

本文以磷钨酸－氯化镁作沉淀剂，速率法测定血清低密度脂蛋白，极低密度脂蛋白结合的r－谷氨酰转移酶（LDL－VLDL－GGT）占总GGT的百分比。对临床26例肝癌、18例肝硬化、22例慢性肝炎、14例其他恶性肿瘤、16例GGT升高的非肝脏疾病及50例健康人进行了检测，结果表明：肝癌组明显高于其他各组，差异显著（P＜0.01）。肝脏疾病升高幅度：肝癌＞肝硬化＞慢性肝炎，且每两者间有显著差异（P＜0.01）。

Methods:Activity of α-HBD in serum and saliva among 88 cases of oral malignant tumor,57 cases of oral cyst,52 cases of pleomorphic adenoma of parotid,62 cases of oral inflammation and 57 healthy men was detected by enzyme speed method.

利用酶速率方法测定88例口腔恶性肿瘤，57例口腔囊肿，52例腮腺多形性腺瘤，62例口腔炎症和57例健康人的血清及唾液中的α-HBD。

Serum ECP were measured with Pharmacia UniCAP system and the lung functions tests were examined with pneumometer.

两组患者在常规治疗基础上给予糖皮质激素：泼尼松30mg/d或相当剂量的氢化可的松。3组研究对象均采用荧光酶标法pharmaciaCAP检测系统测定治疗前、后血清ECP及1s用力呼气容积(FEV1)。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。