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血清酶

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Methods:Prepared multilaminar liposome vaccine by film evaporaqtion combined with lyophilization; Immunized BALB/c mice with the obtained liposomeencapsulated influenza split vaccine and the routine influenza split vaccine, serum antibodies were assayed on weeks 213 by the haemagglutinationinhibition test and ELISA, the HI test for the special antiHA antigen and the ELISA test for the IgG. Protective immunity against intranasal virus challenge was determined at 12 weeks postvaccination.

采用薄膜法与冷冻干燥法相结合的方法制得多层脂质体;用制得的脂质体流感疫苗和普通疫苗分别免疫小鼠,用血凝抑制实验和酶联免疫吸附试验对免后2~13周的小鼠血清的抗体水平进行检测,HI检测其特异性抗体,ELISA检测IgG抗体水平并对小鼠的保护效率进行检测。

Methods Marrow cells from NH mouse were harvested and cultured in α-MEM with 10% fetal bovine serum.The appearance of tartrate-resistant acid phosphatase-staining multinuclear cells and formation of bone resorption pits were measured after 3,6,9 and 12 day exposure to various concentrations of 1,25-2D3 in culture. Results 1,25-2D3 (concentration higher than 10-9mol/L) could induce recruitment of OLCs and their bone resorption activity in a dose-dependent manner.

收集NH小鼠骨髓细胞于含10%胎牛血清的α-MEM培养基中行体外培养,设置不同的1,25-2D3浓度组和给药时间组,并于培养第3、6、9、12天观察记录抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,TRAP)阳性多核巨细胞[或破骨细胞样细胞(osteroblast-like cell,OLC)]以及骨磨片上骨吸收陷窝的数目。

PartⅡ:Effect of bicyclol on hepatocyte apoptosis in alcohol-intoxicated mice and related mechanismsThree consecutive intragastric administration of alcohol(6g/kg)in 12h interval resulted in an obvious liver injury and inflammation in mice,as indicated by the elevation of serum alanine transaminase(2.2 fold of control),increased hepatic COX-2 protein expression(2.2 fold of control),as well as liver pathological changes characterized by myelonic degeneration,vacuolar degeneration and inflammatory infiltration.

双环醇对酒精中毒小鼠肝脏细胞凋亡的保护作用及机制研究小鼠连续三次酒精(6g/kg)灌胃后可导致明显肝损伤,变现为血清ALT水平升高(为对照组的2.2倍),肝脏炎症相关蛋白环氧合酶-2(cyclooxygenase-2,COX-2)表达增加(为对照组的2.2倍),肝组织出现髓样变性、小空泡变性和炎性细胞浸润。

The venous blood was drawn to detect the expression of PMN CD11b, plasma MDA concentration and serum myocardiac enzymes.

在不同时间点采静脉血测定PMN CD11b表达、血浆MDA水平和血清心肌酶水平。

Objective To establish a colorimetric assay for detecting acid alpha naphthyl acetate esterase in serum.

目的 建立比色测定血清酸性α醋酸萘酯酶总活性的方法。

Methods 84 nasopyaryngeal carcinoma patients,57 nasitis patients and 60 healthy control were selected. EBVCA-IgA and EBNA-IgA in serum were determined to evaluated the diagnostic value of the combined determination of the two indexes.

分别采用免疫酶法和ELISA间接法检测60例正常人,57例鼻炎患者和84例鼻咽癌患者血清中EB病毒壳抗原IgA和EB病毒核抗原IgA,评价两项指标联合检测对鼻咽癌的诊断价值。

Serum indicators-urea nitrogen, cholesterol total, alkaline phosphatase manifestly were heightened and some animals' hepatic cells, nephric tubules and mucosa emerged differently damage at histomorphology by giving crude high dose after 2 months.

连续给药2个月,可见生品的高剂量组的血清生化指标的尿素氮、总胆固醇和碱性磷酸酶明显升高,组织形态学检查部分动物的肝细胞、肾小管和胃黏膜均产生程度不同的损伤。

Objective To evaluate the prognostic value of the level of serum neurone specific enolase in patients with small cell lung cancer.

目的 评价血清神经元烯醇化酶水平对小细胞肺癌患者的预后影响。

Objective To investigate the changes of Neurone Specific Enolase concentration in serum of patients with transient ischemia attach and to evaluate its clinical significance.

检测血清神经元特异性烯醇化酶在短暂性脑血发作患者中的变化以探讨其临床意义。

Methods TORCH-IgM,TNF,IL-6 in 56 cases of recurrent spontaneous abortion, 20 cases of early pregnant and 20 cases of nonpregnant women were determind by ELISA.

方法采用酶联免疫吸附法检测56例流产患者、20例早孕妇女及20例非孕妇女血清中TORCH-IgM、TNF、IL-6的水平。

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