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Reproducibility, recovery rate, filate range, interference of haematolysis and lipaemia were evaluated by NCCLC system, and then the results were compared.

用钒酸氧化法、改良J—G法和胆红素氧化酶法测定血清中的总胆红素,按NCCLC评价方案系统评价其重复性、回收率、线形范围及溶血、脂血对胆红素测定的干扰影响,然后对测定结果进行比较。

Tuber Fleeceflower Root serum can postpone senescence of 2BS, its mechanism may be associated with enhancement elimination of free radicals and activation of telomerase.

含何首乌血清对2BS细胞具有抗衰老作用,其机理可能与促进自由基的清除和激活端粒酶活性有关。

Such masses may also focally obstruct the biliary tract and lead to an elevated alkaline phosphatase .

此肿瘤还可引起胆管阻塞,而使血清中碱性磷酸酶含量增高。

Objective To explore the regulation effect of haematoxylin on immunogenic reaction of rats suffered from UC by serum TLC subgroup, IgG and lysozyme level examination.

目的 通过对溃疡性结肠炎大鼠的血清TLC亚群的检测和IgG、溶菌酶含量的测定,探讨苏木对UC大鼠免疫功能的调节作用。

Four field strains of infectious bronchitis viruswere isolated from chicken kidney, trachea and lung of the young layer flocks in some regions of Shaanxi province. Biological characterizations of the virus were investigated by virus pathogenicity to chicken embryo and healthy chickens, hemagglutination test, hemagglutination inhibition test, dwarfing embryonated chicken eggs, electron microscopy and reverse transcription polymerase chain reaction. It demonstrated that allantoic fluid of each passage virus could hemagglutinate chicken red blood cells by treatment with 1% trypsin. The standard positive serum to IBV could inhibited the hemagglutination. The isolates could make respiratory and kidney diseases in chickens.

采集陕西省宝鸡市、扶风县、兴平市和榆林市等地鸡场疑似传染性支气管炎发病鸡病料,通过9~11 日龄非免疫鸡胚进行了病毒的实验室分离和传代,并对分离毒株进行了病毒的血凝性、血凝抑制性、致病性、鸡胚矮小化、电镜特征等生物学特性鉴定,结果表明,4 株分离株经1%胰酶处理后的各代病毒尿囊液均可凝集鸡红细胞,标准阳性血清可特异性的抑制其凝集性,回归试验可复制出与自然发病相同的病例,病毒传代物有明显的致鸡胚矮小化作用,透射电镜下可见有近似球形、直径100 nm左右的冠状病毒粒子,将分离鉴定的4 株IBV 定名为BJ-04、FF-04、XP-03 和YL-04。

Objective : To detect the serum level of triglyceride、total cholesterol、low-density-lipoprotein-cholesterol、plasma viscosity,fibrinogen 、the level of plasma tissue plasminogen activator,plasminogen activator inhibitor-1(PAI-1) and calculate the score of nervous impairment of acute ischemia apoplexy patients ; so as to compre- hend changes of lipid metabolism、hemorheology and fibrinolysis function of AIA patients and it relation of the condition degree, and to investigate relationship between the above-mentioned microcosmic indexes and phlegm blood stasis syndrome.

目的:通过观察急性缺血性中风患者血清甘油三酯、胆固醇、低密度脂蛋白、血浆粘度、纤维蛋白原、血浆组织纤溶酶原激活物及其抑制剂-1(PAI-1)和评定神经功能缺损程度积分以了解急性缺血性中风患者脂质代谢、血液流变性及纤溶系统功能的变化及其与病情程度的关系,并探讨上述微观指标与痰瘀证的关系。

Methods Autologous cord blood serum was used to culture cord mesenchymal stem cells. An enzyme digesting method was used to isolate mesenchymal stem cells from the cord. Morphology, immunotyping and hepatogenic differentiation of these cells were assayed.

分离自体脐血清用于后续细胞培养,利用酶消化法从脐带组织中分离培养间充质干细胞,检测其形态和免疫表型,并采用两步法诱导其向肝细胞诱导分化。

Results Digestive disorder, elevated alanine transaminase and rash were the most common side effects of antiretrovirals, fatigue, loss of hair, sleep disorder, fat maldistribution, peripheral neuropathy and hypocytosis were also commonly seen, yet pancreatitis, color change of skin and lactic acidosis were seldom seen.

结果 消化道症状、血清丙氨酸氨基转移酶升高、皮疹为最常见的艾滋病抗病毒药物不良反应,疲倦、脱发、失眠、脂肪分布异常、四肢肢端麻木、血细胞减少为较常见的不良反应,而胰腺炎、皮肤色素沉着和乳酸性酸中毒则为较少见的艾滋病抗病毒药物不良反应。

Methods: Twenty-eight patients with advanced gastrointestinal tumors entered into this study. Octreotide was administered hypodermically at a dosage of 0.2 mg once even 12 hours, till the disease progressed or the patients became intolerable to the therapy. The CBR was assessed by comprehensive evaluation, the serum level of IGF-Ⅰ was detected by double-antibody sandwich enzyme-linked immunosorbent assay before and one month after treatment.

予28例晚期消化道肿瘤患者奥曲肽0.2 mg,每12h皮下注射1次,直至病情进展或不能耐受治疗为止,综合评估CBR;采用双抗体夹心酶联免疫吸附测检测治疗前和治疗1个月后的血清IGF-Ⅰ水平。

Result Cells growed well in DMEM that contain 15% fetal cattle serum, and inoculative density was 4×10^4cellIml, penicillin and streptomycin concentration was 0.05%, pH was 7.2. It was easy to control and have good digest effect that use Hanks washes the cells three times advance then apply 0.05% trypsin-EDTA-Na2H2O to digest for one minute when convergence degree reaches 95-100%.

结果 含15%胎牛血清的DMEM培养基,接种密度为4×10^4个/mL,双抗浓度为0.5%,pH为7.2;在汇合度达95~100%时,Hanks洗3次,0.05%胰酶-EDTA-Na2H2O消化1min,传代效果最好,条件易于控制,且细胞能顺利生长。

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