血清酶

We have measured the serum levels of total cholic acid in 103 samples of obstructive jaundiced patients and 83 samples of gallbladder stone patients without jaundice by enzymeconjugated colorimetric analysis method.

采用酶偶联比色法测定47例（103例次）阻塞性黄疸和53例（83例次）无黄疸单纯胆囊结石患者血清总胆酸的水平，并探讨其与黄疸程度及肝细胞损害的关系。

A polyclonal antibody against vitellin was prepared in male rabbits and the specific antibody against vitellin was made through absorbtion by male worm homogenate. After purification, HRP was used to mark antibody, then ELISA was set up to detect vitellin content in the Chrysopa septempunctata fat-body, hemolymph and ovary.

提取了大草蛉的卵黄蛋白，免疫家兔，并将所得抗血清经雄虫匀浆液吸收，获得抗卵黄蛋白的特异抗体，经纯化后，用辣根过氧化物酶标记抗体，建立了ELISA法测定大草蛉脂肪体、血淋巴和卵巢中卵黄蛋白含量的方法。

Methods; In APS treatment group and control group, levels of insulin, C-peptide, myocardial enzymes, glycosylated serum protein and angiotensin were measured; Expression levels of chymase and angiotensin-coverting enzyme mRNA were detected by RT-PCR.

检测APS治疗组和对照组DM仓鼠的胰岛素、C肽、心肌酶谱、糖化血清蛋白和血浆。

Results As compared to DM control group, levels of GSP, myocardial enzymes and myocardial Ang Ⅱ were much lower in APS group, while levels of insulin, C-peptide and plasma Ang Ⅱ were the same. Levels of expression of collagen Ⅰ and the ratio of collagen Ⅰ/collagen Ⅲ in APS group were lower than those in DM group. APS group showed lower levels of chymase mRNA expression and activity than those in DM control group, while there was no diference in ACE mRNA expression and activity between the two groups.

结果 APS组血糖、糖化血清蛋白、心肌酶谱和心肌Ang Ⅱ水平较DM组显著下降，胰岛素、C肽、血浆AngⅡ水平和DM组无差异；APS组心肌Ⅰ型胶原表达和Ⅰ/Ⅲ型胶原比值较DM组显著下降；APS组chymase mRNA表达和活性均显著低于DM组，ACE基因的表达和活性与DM组无显著性差异。

Methods In APS group and diabetes mellitus control group, levels of serum glucose, insulin, C peptide, myocardial enzymes, glycosylated serum protein and angiotensin Ⅱ in plasma and myocardial were tested; Immuno-histochemistry was used to measure expression levels of type Ⅰ and type Ⅲ collagen; Expression levels of chymase Mrna and angiotensin-converting enzyme mRNA were detected by using fluorescent quantitative polymerize-chain-reaction; Activities of chymase and ACE were tested by radioimmunoassay.

检测APS治疗组和DM对照组仓鼠的血糖、胰岛素、C肽、心肌酶谱、糖化血清蛋白水平，血浆和心肌组织的AngⅡ含量；免疫组化法检测心肌Ⅰ、Ⅲ型胶原；荧光定量PCR法检测心肌chymase和ACE mRNA表达；放免法检测chymase和ACE活性。

The differences of the biochemical indicators and cnemis compression experiments of all these groups were tested.

目的：通过生化指标和力学实验研究，测定不同周龄SD大鼠血清中钙离子和碱性磷酸酶变化趋势，以及不同周龄大鼠胫骨抗压缩破坏载荷的变化。

Forty-eight rats of 9 months old were randomly divided into the normal group, the suspending group, the suspending load group, the ovariectomizing group and the ovariectomizing load group. The differences of the biochemical indicators and cnemis compression experiments of all these groups were tested.

选用48只清洁级9个月月龄 SD 大鼠，随机分为正常组、悬吊组、去势组和假手术组，以断头的方式处死实验动物，测定血清钙离子和碱性磷酸酶指标和测量整根胫骨在静载压缩破坏情况下的组间差异。

Methods An enzymetic colorimetric method was used to determine uric acid of 2525 middle school students in Shanghai.

采用酶比色法，应用日立－7150型全自动生化分析仪上对上海市2525名中学生的空腹血清进行尿酸测定。

Objective:To study the changes of Creactive protein,Creatine Kinase,Creatine KinaseMB and CKMB/CK on patients with coronary heart disease effectively with salvia mihiorrhiza composita,and their relativity.

目的：探讨应用复方丹参注射液后冠心病患者血清C反应蛋白、肌酸激酶、肌酸激酶同工酶及 CKMB/CK的变化情况及其相关性。

Influence of salvia mihiorrhiza composita on coronary heart disease′s Creactive protein,Creatine Kinase,Creatine KinaseMB

核心提示：目的：探讨应用复方丹参注射液后冠心病患者血清C反应蛋白、肌酸激酶、肌酸激酶同工酶及 CKMB/CK的变化情况及其相关性。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！