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血清酶

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The control group of 30 patients were given routine treatment, while the test group of 34 patients were given routine treatment combined with the injection of erigeron.

视网膜静脉阻塞患者64例64眼分为灯盏花素治疗组34例(50mg×4wk)和对照组30例,采用酶法测定治疗前后血清同型半胱氨酸水平。

A Study of the Relationship Between Serum Esterase Polymorphism and Reproductive Performance of Saba Pig.

撒坝猪血清酯酶多态性与繁殖性能关系的研究。

Seroepidemiologic Studies ;; Measles ;; Rubella ;; Exanthema ;; Antibodies, Viral ;; Fever ;; Disease ;; Immunoglobulin M ;; Enzyme-Linked Immunosorbent Assay

血清流行病学研究;;麻疹;;风疹;;疹;;抗体,病毒;;发热;;疾病;;免疫球蛋白M ;;酶联免疫吸附测定

This study aimed to compare the effeciveness of ELISA and FAVA in detection of antibody against rabies vaccine.

比较酶联免疫吸附试验与细胞培养病毒中和试验检测狂犬病疫苗免疫后血清抗体。

objective to investigate the factors influencing the estrogen production of functional ovarian hyperandrogenism.methods 18 patients with functional ovarian hyperandrogenism were divided into two groups according to the number of the follicles measuring 2~8mm in diameter,one group had 8 to 11 follicles together in two lateral ovaries (named as foh ⅰ),and the other group had 12 to 24 (named as foh ⅱ).eight ovulatory females were recruited as controls.an ovary stimulation test with hcg and hmg was administered to evaluate the level of testosterone and estradiol (e2) in serum.then,the functional state and functional activity of aromatase were calculated.

目的 探讨功能性卵巢高雄患者雌激素合成的影响因素。方法 18例foh,患者分为2组:双侧小卵泡(φ2~8?mm)数量为8~11的为foh ⅰ组(9例),数量为12~24的为foh ⅱ组(9例)。8名正常妇女作为对照组。用人绝经期促性腺激素(150?u)和绒毛膜促性腺激素(5000?u)进行卵巢兴奋试验,试验后3,8,12,18,24?h时检测血清睾酮和雌二醇(e2)水平,计算芳香化酶功能状态和活性。

ABSTRACT:Objective To investigate the glutamic acid decarboxylase-reactive T cells in diabetes mellitus.

目的 探讨糖尿病患者血清中谷氨酸脱羧酶反应性T淋巴细胞的阳性反应及临床意义。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因,并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列,通过对克隆的降钙素序列的比较研究,结果显示同一科的鱼降钙素序列保守性较高,同时,根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究,结果显示在分类学上,分类地位较远的鱼,其降钙素相似性较低,分类地位越接近的鱼,其降钙素相似性越高;我们利用谷胱甘肽S-转移酶(Glutathions S-transferase,GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究,应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索,并将纯化的融合蛋白作为抗原,获得了高效价的兔抗鲑鱼降钙素免疫血清;生物活性研究表明,大肠杆菌表达的融合蛋白具有显著的降血钙作用。

Here we found the optimum situation to determine LAP: the concentration of Tris-HCl is 125 mmol L^(-1) and the optimum pH is 7.2, temperature is 37℃, under this condition, Michaelis constant is 0.15 mmolL^(-1), ground substance concentration is 6.0 mmol L^(-1), with 157 health people serum samples (81 from male, 76 from female),x± s=35.7 UL^(-1)±8.2 UL^(-1), we established the method and reference value for clinical application and automatic analysis.

测定亮氨酰氨基肽酶的最适条件:选用浓度为125mmolL^(-1)的Tris-Hcl缓冲液,最适pH为7.2,温度取37℃,得出在此实验条件下的米氏常数Km为0.15mmolL^(1),亮氨酰对硝基苯胺浓度取6.0mmolL^(-1),取健康体检者血清157例(男81例,女76例),测得x±s为35.7UL^(-1)+8.2UL^(-1),建立了适合于临床应用及有动生化分析仪的方法和参考值。

Penicillinase, hematein and nano gold colloid were immobilized,as a modified membrane,on a plane Pt electrode by glutaraldehyde-bovine serum albumin.

用戊二醛-牛血清白蛋白交联法将青霉素酶、纳米金胶和氧化苏木精同时修饰在铂电极上,制成电流型电化学生物传感器。

In a pilot hennery, chitosan agents were applied to approach their functions on meat chicken's anti-oxidation activity and production performance by determining the superoxide dismutase and glutathione peroxidase activities and malondialdehyde concentration in blood serum, and the immune organ index, percentage of abdomen fat, and ratio of chicken meat mass to feedstuff. The experiment as designed as 6 groups, with 100 one-day-old chickens for each.

为了探讨壳聚糖对家禽肉鸡抗氧化能力及生产性能的作用,采用1日龄黑脚大麻鸡6组各100只进行分组实验,喂食壳聚糖复合剂,测定了肉鸡血清中超氧化物歧化酶活力、丙二醛含量、谷胱甘肽过氧化物酶活力、免疫器官指数、腹脂率和料重比等指标。

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