血清酶

In vitro, we cultured human and rabbit retinal Müller cells by way of mixed enzyme digestion and tissue adherence, using human retina of an abortus of 6 months and healthy rabbit.

取孕6个月引产胎儿，死后2h内无菌条件下摘取眼球，剥取视网膜组织，混合酶消化法培养人视网膜Müller细胞；取健康新西兰大白兔，死后以最快的速度无菌条件下摘取眼球，组织块贴壁法培养兔视网膜Müller细胞；利用含胎牛血清的高糖DMEM作为培养液，不添加生长因子。

Objective To explore the diagnostic value of serum adenosine deaminase activity in pulmonary tuberculosis.

目的 探讨血清腺苷脱氨酶对肺结核的诊断价值。

Methods Observation on the anti-inflammatory effects of 11-sodium deoxyglycyrrhetinic acid in three models i.e. induced mice's ear swelling, chronic granuloma by filter paper and air sac synovitis. The NOS activity and contents of PGE2 in mice serum and inflammatory humour were measured.

通过二甲苯致小鼠耳片肿胀、纸片致小鼠慢性肉芽肿及气囊性滑膜炎3个模型观察11-脱氧甘草次酸钠的抗炎作用，并测定小鼠血清和炎症液中前列腺素E2(PGE2)的含量和一氧化氮合酶活性。

To study the diagnostic value of serum alanine aminopeptidase activity in both liver and biliary tract diseases.

为观察血清丙氨酸氨基肽酶水平对肝胆疾病的临床诊断价值。

The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.

采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型，取健康大鼠每日灌服不同剂量的鲜姜有效部位(200，400，800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1)，取含药血清作为受试药物，用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力；逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平；酶联免疫吸附法测定MCP-1蛋白表达水平。

IL-6,TNF-α levels in sera were determined by means of enzyme linkage immuno-sorption assay, 3H-TdR release assay was used to measure NK cell activity, and APAAP bridge enzyme label assay was used to type subpoppulation of T lymphocytes.

用酶联免疫吸附法检测血清IL-6、TNF-α浓度，3H-TdR掺入法检测NK细胞活性，APAAP桥联酶标法进行淋巴细胞亚群分型。

The results showed that ①after the treatment with 0. 3% Triton X-100 5 min or standard binding buffer 20 min,the proteins on NCM could be detected with first specific antiserum from the titer 1:100 diluted to 1: 1000;②the blocking effect of Tween 20 was more effective than that of other blockings;③both HRP-labelled conjugate and colloid gold-labelled c...

结果表明：①经（TritonX－100）＝0.3％，5min和SBB20min的蛋白质复性处理后，可使第一免疫血清稀释度1：100改为1：1000使用；②应用（Tween20）＝0.05％作为封闭液效果最好；③金标法及酶标法都可用于Western印迹技术，酶标法主带清晰，而金标法灵敏度高。

The results show the agglutinative activity, SOD activity, CAT activity and lysozyme activity in serum of Cyprians carpio increased in contrast to that of control group. The activity of fed lectin from Porphyra yezoensis group was 3.0, 0.8, 3.9 and 1.0 times, respedctively, as much as that of control group. The activity of fed lectin from Undaria Pinnatifida group was 1.0, 0.4, 3.0 and 0.7 times, respectively, as much as that of control group. The activity of infusing lectin from Undaria pinnatifida group was 3.0, 0.3 and 1.8 times, respectively, as much as that of control group. The activity of infusing lectin from Porphyra yezoensis group was 3.0 and 0.7 times, respectively, as much as that of control group.

实验结果表明，投喂和灌喂凝集素后，鲤血清中凝集素、超氧化物歧化酶、过氧化氢酶、溶菌酶活性与对照组相比都有提高，其中投喂条斑紫菜凝集素组比投喂对照组依次提高3.0倍、0.8倍、3.9倍、1.0倍；投喂裙带菜凝集素组比投喂对照组依次提高1.0倍、0.4倍、3.0倍、0.7倍，灌喂裙带菜凝集素组比灌喂对照组依次提高3.0倍、0.3倍、1.8倍；灌喂条斑紫菜凝集素组比灌喂对照组依次提高3.0倍、0.7倍。

The AKP activities of experimental groups in serum and haemocytes were much higher than control grou...

血清和血细胞实验组的碱性磷酸酶活力仅在1h时，溶菌酶活力分别在1h和15 h时显著高于对照组。

Methods Phosphotyrosine was first combined with hemocyanin from keyhole limpets. Then, rabbits were immuned with the complete antigen and antisera were gained. The ELISA system consisted of purified antiphosphotyrosine antibody as first antibody and sheep antirabbit lgG-HRP as second antibody. Results CV within the batch was 8.4%.

应用合成的磷酸酪氨酸-钥孔帽贝血蓝蛋白免疫家兔得到抗血清，将纯化的抗磷酸酪氨酸抗体作为第一抗体，羊抗兔Ig酶结合物作为第二抗体，建立双抗体夹心酶联免疫吸附测定法。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！