血清酶

Methods:The level of E2 was evaluated by Progesrone Serozyme,the expression of ER was tested by Immunohistocheminal assay,ER gene expression was tested by RT-PCR in 35 cases with mammary hyperplasia of stagnation of Liver-qi type,15 cases with mammary hyperplasia of disorder of thoroughfare vessel and conception Vessel type and 30 cases of healthy people were taken as control group.

分别用磁分离酶联免疫测定法、免疫组化法和逆转录—聚合酶链反应法，检测35例肝郁气滞型乳腺增生病患者及15例冲任失调型患者和30例正常人黄体期血清雌激素水平（E2 ）、乳腺组织雌激素受体及受体基因的表达。

We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术，分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因，以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，分别自43例、51例慢性HBV感染患者血清中扩增出目的片段，构建质粒Teasy-BCP和Teasy-pre S2，酶切鉴定后，采用聚丙烯酰胺凝胶电泳技术展示缺失突变，再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

The strategy was firstly optimized and evaluated by using the tryptic peptides of bovine serum albumin. Then the method was applied with a relatively complicated sample from a five standard protein mixture.

首先以牛血清白蛋白的酶切肽段为模型，对富集条件进行了优化和考察，并在此基础上通过5种蛋白质酶切肽段混合物的富集对该方法进行了验证。

Fourteen weeks later all the rats were sacrificed underwent serological test and immunohistochemical stain of liver. Results Serum level of γ-Glutamyltransferase, alkaline phosphatase, bile acid were significantly higher in DEN exposure rats than those in normal rats, and radiated rats were lower than unradiated rats, but has no significant difference.

结果诱导的肝癌大鼠，血清中γ-谷氨酰转肽酶、碱性磷酸酶、胆汁酸较正常大鼠组明显升高，毫米波照射肝癌大鼠γ-GT、AKP、BA与肿瘤对照组相比略下降，统计学上各组间无明显差异。

The LDH total activity and its isoenzymes patterns of cancer tissue in untreated and recurreut NPC groups were different from those in unrecurrent NPC and benign lesions of heads and necks.

鼻咽癌放疗后复发组血清和组织LDH总酶活力及其同工酶谱均与鼻咽癌初诊组相似，而未复发组则接近于良性病变组，与复发组显著不同。

Contents of heat chock protein 70 （expressed with integral scanning area） and blood uria nitrogen as well as activities of alanine aminotransferase and aspartate aminotransferase were measured in each group.

将各组大鼠麻醉处死取血，分离血清，测定各组大鼠热休克蛋白70、尿素氮含量及丙氨酸氨基转移酶、天冬氨酸氨基转移酶活性。

The results showed that the electrophoretogram patterns of isozymes, serum and muscle protein of Bumper crucian carp were the same as those of its female parent and significantly different from its male parent.

结果发现：异源子代的血清、肌肉蛋白和同工酶的电泳图谱与母本彭泽鲫相同而与父本海鲤显著不同，推测异源精子的作用可能是在DNA水平上对后代产生影响，这种影响没有表现在成体同工酶的表达上。

Itamin D from the skin and diet is metabolized in the lier to 25-hydroxyitamin D (Figure 1), which is used to determine a patient's itamin D status1,2,3,4; 25-hydroxyitamin D is metabolized in the kidneys by the enzyme 25-hydroxyitamin D-1-hydroxylase (CYP27B1) to its actie form, 1,25-dihydroxyitamin D.1,2,3,4 The renal production of 1,25-dihydroxyitamin D is tightly regulated by plasma parathyroid hormone leels and serum calcium and phosphorus leels.1,2,3,4 Fibroblast growth factor 23, secreted from the bone, causes the sodium–phosphate cotransporter to be internalized by the cells of the kidney and small intestine and also suppresses 1,25-dihydroxyitamin D synthesis.5 The efficiency of the absorption of renal calcium and of intestinal calcium and phosphorus is increased in the presence of 1,25-dihydroxyitamin D (Figure 1).2,3,6 It also induces the expression of the enzyme 25-hydroxyitamin D-24-hydroxylase (CYP24), which catabolizes both 25-hydroxyitamin D and 1,25-dihydroxyitamin D into biologically inactie, water-soluble calcitroic acid.2,3,4

从皮肤和食物来的维生素D在肝中代谢为25-羟基维生素D（图1），被用来决定病人体内维生素D情况的1，2，3，4；25-羟基维生素D在肾中被25-羟基维生素D1羟化酶（CYP27B1）转变为有活性的1，25-二羟基维生素D 。1，2，3，4由肾产生1，25-二羟基维生素D是被血浆甲状旁腺激素和血清钙，磷水平紧密调节。1，2，3，4由骨分泌的成纤维细胞生长因子23使钠磷协同转运蛋白被肾和小肠细胞内化及抑制1，25-二羟维生素D合成。5 在1，25-二羟基维生素D作用下肾和小肠吸收钙及磷的效率增高(图1)。2，3，6 它也包括25-羟四- 24 -羟化酶的表达(CYP24)，且将1，25二羟基维生素D和25羟基维生素D异化成无生物活性，水溶性的维生素D3-23羧酸。2，3，4

Enzyme activity assay demonstrated that the Trx-BTGase expressed in E. coli could be functional in polymerizing other proteins, and no influence on the biological activities of BTGase to polymerize BSA was found in case of the thrombin cleavage of this fusion protein.

酶活性分析表明表达的Trx-BTGase融合蛋白具有交联蛋白的活性，并发现Trx-BTGase融合蛋白和经凝血酶酶切后得到的BTGase单体都能催化牛血清白蛋白的聚合反应。

Serum alt, ast, bile acid and albumin levels were measured with automatic biochemistry analysator during the procedure.

采用ccl4造模，全自动生化分析仪检测血清丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、胆汁酸及白蛋白水平；he染色和vg染色观察肝脏病理及纤维化面积的变化，分析黄芪对肝功能及肝纤维化形成的影响。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！