血清酶

Applied rabbit anti-5-HT and mouse monoclonal anti-TPH antisera, the localization of 5-HT and its synthesizing enzyme TPH in the visual system, midbrain, and suboesophageal ganglion of the beetle Harmonia axyridis (Coleoptera: Coccinellidae) was studied by Streptavidin-Peroxidase immunohistochemical method on Colophony-Paraffin embedded serial sections.

运用树脂石蜡组织包埋切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶双染法，采用兔抗5-HT抗血清和鼠抗TPH单克隆抗体，初步构建了异色瓢虫Harmonia axyridis视觉系统、脑中部及咽下神经节5-HT及其合成酶TPH抗原系统的免疫组织化学反应模式。

Applied rabbit anti-5-HT and mouse monoclonal anti-TPH antisera, the localization of5-HT and its synthesizing enzyme TPH in the visual system, midbrain, and suboesophagealganglion of the beetle Harmonia axyridis (Coleoptera: Coccinellidae) was studied byStreptavidin-Peroxidase immunohistochemical method on Colophony-Paraffin embeddedserial sections. An overall observation reveals that the brain structure of the beetle is relativelydistinct.

运用树脂石蜡组织包埋切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶双染法，采用兔抗5-HT抗血清和鼠抗TPH单克隆抗体，初步构建了异色瓢虫Harmoniaaxyridis视觉系统、脑中部及咽下神经节5-HT及其合成酶TPH抗原系统的免疫组织化学反应模式。

Molecular weight and molecular weight distribution and dissociative sulfate content were determined by these methods respectively.

结果表明在所选择的实验条件下，916对溶菌酶和胰蛋白酶是稳定的，可以推定，在血清中916不会被这两种酶降解。

Methods The concentrations of serum PCⅢand angiotensinⅡ were measured by radioimmunoassay in 43 patients with AMI, the serum concentrations of creatine kinase isoenzymes MB also were measured by enzym immounoassay at the same time.

采用放免法检测 4 3例AMI患者血清PCⅢ、血管紧张素Ⅱ(angiotensinⅡ，AngⅡ)水平，酶免疫测定法检测肌酸磷酸激酶同工酶MB水平。

The changes of enzymic activity or zymogram might reflect the development of avian TD.

组织及血清中酶活性及同工酶谱的变化反映了TD的发生、发展。

The article reviews the advanced research on screening sheepbreeds with high resistance against fasciolosis. Screening sheep breeds with high resistance against fasciolosis has been tested by measuring parameter of plasma such as packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin concen tration, glutamate dehydrogenase, gamma glutamyl transferase and aspartate trans ferase and percent take of metacercariae. High resistance of Indonesian thin tail sheep against Fasciola gigantica has been confirmed. The mechanism of high res istance and the time of killing Fasciola gigantica has also been described.

本文综述了抗片形吸虫病绵羊品种的研究概况，通过红细胞压积、红细胞平均体积、红细胞平均血红蛋白浓度、血清谷氨酸脱氢酶、γ谷氨酰胺转移酶、天冬氨酸转氨酶等血液学参数，囊蚴感染成活率的测定等方法来筛选抗片形吸虫病的绵羊品种，研究证明印度尼西亚细尾绵羊对大片吸虫感染具有较高的抵抗力，并概述了其抗虫感染的机制及杀虫作用时间。

Methods 2BS cell lines were treated with Tuber Fleeceflower Root serum. The activity of c-myc mRNA was detected by semi-quantitive RT-PCR. A TRAP-ELISA method was used to measure the telomerase activity.

采用含何首乌血清对2BS细胞株进行处理，用黄嘌呤氧化酶法检测SOD活性，RT-PCR检测c-myc mRNA表达，TRAP-ELISA检测端粒酶活性。

In this study,an indirect immunoperoxidase technique based on hyperimmune serum was developed to detect avian nephropathogenic infectious bronchitis virus in paraffin_embleded tissue sections of immune organs.

以鼠抗鸡肾型传染性支气管炎病毒的高免血清为一抗，辣根过氧化物酶标记的羊抗鼠IgG为二抗，建立了检测石蜡切片肾型IBV抗原的间接酶标抗体染色技术。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂（β-甘油磷酸钠，地塞米松，维生素C）、成脂肪细胞诱导液（地塞米松，甲基异丁酸黄嘌呤，牛胰岛素，吲哚美辛）及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化，经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物（碱性磷酸酶、骨钙素mRNA、钙结节）、脂肪标志物（脂滴、PPARγ-2mRNA）、以及类神经标志物（尼克氏体、神经烯醇化酶、神经丝蛋白）。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！