血清酶

Methods Three-dimensional gels of type I collagen containing rabbit keratocytes were incubated in the presence of different concentrations of exotoxin A (0.1,1.0,10 mg·L-1),cultivated for 24 h at 37℃, the change of keratocytes in morphology was observed under the light microscope,and the amount of lactate dehydrogenase was measured by enzyme linked immunosorbent assay.

含兔角膜基质细胞（1×105·mL-1）三维胶原凝胶表面添加提纯的不同浓度绿脓杆菌外毒素A（0.1、1.0和10 mg·L-1）后，放入无血清MEM培养液中37℃培养24 h，采用光学显微镜观察培养的兔角膜基质细胞形态学变化，采用酶联免疫吸附法检测损伤的兔角膜基质细胞释放的乳酸脱氢酶含量。

The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板，根据其鞭毛区与其它细菌高度同源性设计引物，利用PCR方法扩增鞭毛蛋白基因flic片段，将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定，再将其亚克隆与载体pGEx-kG分别双酶切和连接，构建重组表达载体pGEX-flic，并将其转化大肠杆菌工程菌BL21，进行原核表达。

The method uses CCl4 revulsive small rat liver injures a model, medicaments is preventive experiment, through determining transaminase of serum cereal third , millet straw and liver organization third 2 aldehyde content, yellow purine oxidizes peptide of pleasant of Guang of enzymatic, cereal is reductive enzymatic active and liver tectology change are index, observation grackle extracts the protective effect that content injures to liver.

方法采用CCl4诱导小鼠肝损伤模型，药物预防性实验，通过测定血清谷丙转氨酶、谷草转氨酶和肝组织丙二醛含量、黄嘌呤氧化酶、谷胱甘肽还原酶活性以及肝组织形态学变化为指标，观察白头翁提取物对肝损伤的保护功能。

Methods ADA was examined by enzyme in 78 cases of tuberculous hydrothorax and 30 cases of malignant hydrothorax.

对78例结核性胸腔积液和30例恶性胸腔积液标本进行腺苷脱氨酶测定，同时测定血清腺苷脱氨酶活性。

Hypoxanthine and a uricase inhibitor were combined into the model drug to work out the rat＇s animal model for hyperuricemia. A comparison was made between the effect of hypoxanthine and that of the model drug on the subject＇s serum uric acid levels. Also, such factors as the dose effect and time effect of the model-building drug were investigated.

采取合用次黄嘌呤和尿酸酶抑制剂为模型药物，制备一种尿酸生成增多型大鼠高尿酸血症动物模型，比较了单用次黄嘌呤及合用尿酸酶抑制剂对受试动物血清尿酸水平的影响，并对造模药物的量效、时效关系等因素进行了考察。

Objective To observe the fluctuation of uric acid in serum on mouse after replicating model by using hypoxanthine and uricase inhibitor. Make certain the most proper approach and solvent for using uricase inhibitor.

目的 本实验考察次黄嘌呤、尿酸酶抑制剂复制大鼠代谢性高尿酸血症模型，观察造模后受试动物血清尿酸的变化，以确定尿酸酶抑制剂造模的最佳造模途径和溶剂。

Methods Acute liver impairment models were made by intraperitoneal injecting 0.1 % CCl4(10 mL/kg).AST and ALT were used as indexes to observe liver protection effect of alcohol extract South Wuweizi steamed with vinegar comparing with raw Wuweizi.

方法腹腔注射0.1%的CC l4花生油溶液(10 mL/kg)制作小鼠急性肝损伤模型，以血清中天门冬氨酸氨基转移酶及丙氨酸氨基转移酶为指标，观察比较生品南五味子和醋蒸南五味子乙醇提取物对小鼠肝脏的保护作用。

After treatment for 4 weeks, the parameters, including blood glucose, glycosidase, maltase, total cholesterol, triglyceride, were determined.

复方中药糖脂清各剂量组，给药前与给药4周后相比各剂量组均可降低血糖，同时降低KK-Ay小鼠小肠的蔗糖酶与麦芽糖酶的活性、血清中总胆固醇和甘油三酯的含量。

Objective:To study LDH level and its isoenzymes in sera and neoplastic tissues of patients with nasopharyngeal carcinoma .

目的：研究鼻咽癌患者血清和癌组织中乳酸脱氢酶及其同工酶的水平。

61Healthy persons were selected as healthy group. The serum concentration of TGF-β1 was measured by ELISA method; nitre oxide was measured by nitrit reductase method, plasma Ang II was measured by radioimmunoassay.

对高血压病人和61例健康对照者应用酶联免疫吸附法测定血清TGF-β_1浓度，硝酸还原酶法测定NO的浓度，放射免疫分析法测定Ang Ⅱ水平。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。