血清酶

The thymuses, spleens, sera and anticoagulant blood were collected after the rats were killed, to carry out these works:(1) Zinc concentrations were analyzed by atomic absorption spectrophotometry;(2) Body weight and the weight of thymuses and spleens were measured, the organ index were calculated;(3) Changes in pathology of thymus and spleen were observed;(4) Using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thmocytes and spleen lymphocytes was checked;(5) The expression of bcl-2、bax mRNA in thymus and spleen were detected by RT-PCR (reverse transcription polymerase chain reaction);(6) The expression of p56〓, a signal transduction protein in thymus and spleen were detected by immunohistochemistry;(7) Two-color cytofluorometric analysis was used to assess the expression of CD4, CD8, CD45RA and CD45RC in peripheral blood lymphocytes.

动物处死后留组织、抗凝血及血清，进行以下检测：(1)原子吸收法测血清锌浓度；(2)测胸腺、脾脏脏器重量并与大鼠体重比较，计算脏器指数；(3)HE染色对胸腺、脾脏进行病理观察；(4)原位末端标记法测胸腺、脾脏中淋巴细胞的自发凋亡，并通过图像分析进行比较；(5)逆转录聚合酶链式反应检测凋亡调控基因bcl-2、bax mRNA在免疫器官中的表达；(6)免疫组化方法检测信号转导蛋白p56〓在免疫器官中的表达；(7)流式细胞术分析外周血淋巴细胞中CD4+、CD8+、CD45RA+及CD45RC+CD4+、CD45RC-CD4+细胞的数量和比例。

The results showed that: 1 SOD and GSH-PX activity of hepatopancreas and serum were significantly or extremely significantly increased when 5% or 10% alfalfa meal was added to the diet, whereas the MDA content of the two groups decreased. 2 The concentration of TC and TG in serum, hepatopancrac and muscle dropped as the amount of alfalfa meal increased.

结果显示，1添加5%和10%的苜蓿草粉能显著或极显著提高黄河鲤鱼血清和肝胰脏中超氧化物歧化酶和谷胱甘肽过氧化物酶活力，同时也能显著或极显著降低丙二醛含量，说明苜蓿草粉能有效地提高黄河鲤鱼的抗氧化能力。2血清、肝胰脏和肌肉中总胆固醇和甘油三酯含量都随着苜蓿草粉添加量的增加依次降低。

Experiment 3: By the 30d feeding method of toxicology in vivo test, it was detected that the effect of olaquindox on blood corpuscle, immunity of erythrocyte, antioxidase system activity of blood serum, some biochemistry item of blood serum, histopathology, subcellular structure and deletion of liver mitochondria DNA and so on, Based on Kunming mice divided randomly into 6 groups .

试验三：采用毒理学体内试验30d 喂养法，将小鼠随机分成6组，检测喹乙醇对血细胞、红细胞免疫功能、血清抗氧化酶系活性、血清多种生化指标、组织病理学、亚细胞结构及肝脏线粒体 DNA 缺失等产生的影响。

Objective To investigate the changes and significances of neurocytes apoptosis and serum nerve growth factor after cerebral contusions.

目的 探讨大鼠脑挫裂伤后海马区神经细胞凋亡及血清神经生长因子的变化及意义方法按照改良Feeney自由落体脑损伤模型方式制作大鼠左顶叶脑挫裂伤模型，应用酶联免疫吸附法和原位细胞凋亡法，分别对脑挫裂伤后0.5，1，3，6，12，24，72，120，168，240h各时相点血清NGF含量及脑组织细胞凋亡进行检测。

blood serums of the crowd are collected, antibody corresponded to the four virus examined by elisa、pa and colloidal gold strip. western blot is the confirmation test of positive for antibody of hiv. result: in the 3852 cases of paid blood donor there are 7 curriers of hiv are detected and they are all paid blood donor in past.

采集有偿献血员的血清，通过酶联免疫吸附实验、明胶颗粒凝集实验和胶体金实验来检测血清中的这四种传染病病原体的相应抗体，抗－hiv阳性确认实验是蛋白印迹实验。

Then, the hapten LBc and LBs was conjugated to bovine serum albumin with CDI method to form immuno-antigen; the haptens LBc, LBs and LBy was conjugated to ovalbumin with mixed anhydride method to form the the coating antigens.The new zealand rabbits were immunized by conjugate of LBc-BSA and LBs-BSA, and titres of anti-bifenthrin serum (5.12×10~4, 3.2×10~5) were determined by non-competitive indirect enzyme-linked assay procedure.

通过碳二亚胺法将LBc和LBs交联于牛血清蛋白作为免疫抗原，通过活泼酯法将分别LBc、LBs和LBy交联于卵清蛋白作为包被抗原，LBc-BSA和LBs为免疫原制备了联苯菊酯的兔抗血清，非竞争间接酶联免疫吸附分析方法测得其效价达5.12×10~4和3.2×10~5。

Objective To analyze HIV serotypes and find the sensitivity of ELISA to detect antibodies against different serotypes of HIV.

目的 分析HIV的血清型并比较不同HIV抗体酶联免疫诊断试剂检测不同血清型HIV抗体的敏感性。

Methods Several known quality contro1 blood serums and 4 2l4 unknown blood serums or blood plasms of patients were tested by both DIGFA ca1ories and EIA. The sensitivity, specificity, physical proportics and agreement of the calories were assessed.

对多份质控血清和4 214份患者血清或血浆分别采用三个厂家的DIGFA试条和酶免疫法进行检测，以EIA技术检测结果为参照对DIGFA检测试条的灵敏度、特异性、检测效能和物理性能等参数进行评价。

Using dual energy X-ray bone density instrument XR-36 model to detect the bone mineral density of the left femur of the rats. Using the strain test system, we examined the biomechanics index according the tension and three-spot bending test; we also examined the bone calcium of the lumbar vertebra through plasma emission spectroscopy . We examined the serum calcium and magnesium by using the nomal biomechanics method,and examined the serum acid phosphatase and osteocalcin by using the Elisa method.

使用NORLAND公司XR-36型双能X线骨密度仪，采用小动物骨密度测定软件，测定大鼠左后肢股骨的骨密度；使用应变测试系统，按股骨拉伸和三点弯曲试验，测定股骨生物力学指标；以等离子体发射光谱法测定腰椎骨骨钙含量；以常规生化法测定血清钙、镁含量及血清酸性磷酸酶含量；以Elisa法测定骨钙素含量。

The beginning of the two easily confused, but has a history of exposure to hepatitis, after dynamic observation, jaundice early serum aminotransferase increased, jaundice more than 2 ~ 3 weeks after the station began to dissipate, serum alkaline phosphatase values do not rise.

初起两者易混淆，但肝炎有接触史，经动态观察，黄疸初起时血清转氨酶增高，黄疸多在2～3周后逐渐消退，血清碱性磷酸酶多不高。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。