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血清酶

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Key words] Thyroid peroxidase antibody;Autoimmune thyroidism disease;Factors inducting into disease

性别、年龄、血清TPOAb滴度均与血清甲状腺过氧化物酶抗体阳性患者甲状腺疾病产生有一定的相关性。

Of them 54 were in wheezy disease group, and 20 in control group. Sandwich ELISA was used to measure the level of serum tIgE, and EUROLINE was used to measure serum sIgE.

喘息性疾病组54人,对照组20人;采用酶连免疫吸附方法检测血清tIgE,采用欧蒙印迹法检测血清sIgE。

Methods: Blood samples were taken from 40 RRTI children (26 males and 14 females, mean age of 3.68±1.47 years) and 35 normal children. Enzyme linked immunosorbent assay method was used to detect the levels of serum tIgE and Western blotting method was used to detect positive percentage of sIgE.

RRTI患儿40例,对照组健康儿童35例,采用酶联免疫吸附法检测血清tIgE水平,欧蒙印迹法检测血清吸入与食物组抗原sIgE抗体阳性率。

Objective To study the content of uric acid in serum, to screen the uricemia patients, and to avoid the diseases caused by uricemia. Methods An enzymetic colorimetric method was used to determine uric acid of 2 525 middle school students in Shanghai.

目的 了解中学生血清尿酸含量,以便尽早发现高尿酸血症患者,避免由此引起的疾病方法采用酶比色法,应用日立-7150型全自动生化分析仪上对上海市2 525名中学生的空腹血清进行尿酸测定。

Eventually, the highly specific anti-melamine polyclonal antibodies were acquired from antiserums through the purification of affinity chromatography.

采用酶联免疫吸附法测定各血清的效价,最终以亲和层析法纯化血清获得高特异性的抗体。

Methods Using a combinatorial approach of enzymatic digestion and mechanical separation to allow isolation and culture of GER cells from P1 rat cochleae.

方法取生后1d大鼠耳蜗,利用机械分离和酶消化相结合的方法分离出纯GER细胞,分别于无血清和含10%胎牛血清的DMEM培基中进行培养,观察细胞生长特性,并对培养物进行溴脱氧尿苷、鬼笔环肽、Z01、钙视网膜蛋白及肌球蛋白VIIa免疫组化鉴定及扫描电镜观察。

The serum-free medium is obtained by: the purified human transferring was directly dissolved in the DMEM. Following detoxicating, BSA, cholesterol, insulin and hydrogen peroxidase were directly added in the DMEM, and diluted to a needed concentration.

所用无血清培养基血清替代物是将纯化人转铁蛋白直接溶于DMEM培养基中,再将去毒后的BSA、超声波粉碎后的胆固醇、胰岛素及过氧化氢酶直接加入DMEM培养基中稀释至所需浓度。

Methods: The model of acute myocardial ischemia in rats was developed by ligaturing the left descending anterior coronary artery. Forty male Wister rats were divided into normal control group, ischemia model group, Xuesetong group and Verapamil group. The effect of Xuesetong on the CPK, LDH, SOD and MDA in serum and myocardium and myocardial infarct range was observed.

采用结扎大鼠冠状动脉左前降支复制出大鼠心肌缺血再灌注损伤模型。40只Wister大鼠分为正常对照组、模型组、血塞通组及维拉帕米组,观察血塞通对大鼠血清磷酸肌酸激酶、乳酸脱氢酶、血清及心肌组织超氧化物歧化酶和脂质过氧化物丙二醛及对心肌梗死范围的影响。

MethodShengneng reagent and Shenneng control serum Ⅰ,Ⅱ(Lot NO.3229, Lot NO.4339) and patients' serum were used to evaluate the precision, linearity, accuracy and the schlepped polluting rate of 7180 biochemistry autoanalyzer by detecting these items as alanine aminotransferase, total protein, calcium, glucose, creatine Cre) and triglycerides.Hitachi 7600 autoanalyzer was used as the control analyzer to evaluate the correlation.

采用上海申能试剂,申能质控血清Ⅰ、Ⅱ(批号NO.3229,NO.4339)和病人血清,选定包括丙氨酸胺基转移酶、总蛋白、葡萄糖、钙、肌酐、甘油三酯在内的六个项目对7180进行精密度、线性、准确度及携带污染率的评价,并且与日立7600型生化仪进行相关性评价。

MethodShengneng reagent and Shenneng control serum Ⅰ,Ⅱ(Lot NO.3229, Lot NO.4339) and patients' serum were used to evaluate the precision, linearity, accuracy and the schlepped polluting rate of 7180 biochemistry autoanalyzer by detecting these items as alanine aminotransferase, total protein, calcium, glucose, creatine Cre) and triglycerides.

采用上海申能试剂,申能质控血清Ⅰ、Ⅱ(批号NO.3229,NO.4339)和病人血清,选定包括丙氨酸胺基转移酶、总蛋白、葡萄糖、钙、肌酐、甘油三酯在内的六

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