血清酶

Methods The serum sICAM-1 was measured by ELISA in 20 patients with pollinosis and in 2 0 healthy persons as normal control group.The serum tIgE was analyzed with the P harmacia Uni-CAP System for two groups but Artemisia pollen-specific IgE was a ssayed only for patents group.

对20例花粉症患者及20名健康人采用酶联免疫吸附实验测定血清sICAM-1，用荧光免疫法测定血清tIgE和蒿草花粉特异性IgE(W6)。

IGF-1 concentration in serum was measured with RIA. Protein concentration in urine was measured with ELASE. Urinary protein excreted in one day was measured with ponceau S. Total protein and albumin level in serum were measured with automatic biochemistry appliance.

以放射免疫法测血清IGF-1水平，用酶联免疫吸附试验测尿微量白蛋白浓度，S-丽春红测24h尿蛋白定量，全自动生化仪测量血清总蛋白和白蛋白。

To establish indirect ELISA method for detection of ribonuclease inhibitor antigen in serum and to analyze the dynamic change of RI in the blood of mice transinfected with RI gene.

建立间接ELISA法测定血清中核糖核酸酶抑制因子（ribonuclease inhibitor，RI）含量的方法，并测定在转RI基因小鼠血清中RI的表达情况。

Methods The RCAⅠidentifying Terminal Galβ14 GlcNAc glycoprotein was acquired by RCAⅠ(Ricinus communis agglutininⅠ) affinity chromatography method from nor mal person serum and was marked by the horseradish peroxidase.133 tumor patients,64 benign disease patients and 240 normal person serums were detected by the subtraction of agglutinin.

应用RCAⅠ亲和层析法从正常人血清中获得RCAⅠ相关末端Galβ14GlcNAc糖蛋白，用辣根过氧化物酶标记，采用凝集素差减法，对133例肿瘤患者，64例良性疾病患者和240例正常人血清进行检测。

In order to establish a sensitive immuno-PCR assay for detecting serai anti-P53antibodies, investigate the relation of anti-P53 antibodies in patients with breast carcinoma to P53 protein expressions in tissue and clinical significances and afford a practical tool in diagnosis of breast carcinoma clinically. SaHIgG was coupled to PNCa DNA by chemical cross-linker PEI and SaHIgG-DNA probe was constructed.

本研究建立了血清抗P53蛋白抗体免疫聚合酶链反应(Immuno-polymerase chain reaction，免疫PCR)检测方法，探讨了乳腺癌患者血清中抗P53蛋白抗体与组织中P53蛋白表达之间的关系及临床意义，旨在为临床乳腺癌P53蛋白检测提供实用的替代工具。

The model groups were fed with different-dose lycopene [10, 15, 20 mg/] for 12weeks. Then related indexes including length bone mineral density, bone mineral, serum estradiol, sertum ALP and uterus index were analyzed.

结果 番茄红素高[20mg/]、中[15mg/]、低剂量组[10mg/]、尼尔雌醇组[1.05mg/kgbw]能对抗骨密度、骨矿含量、血清雌二醇的下降、血清碱性磷酸酶的升高，与模型组比较，P.05；番茄红素各剂量组、尼尔雌醇组能对抗子宫指数的减轻，与模型组比较，P.05。

Methods VEGF in the serum of 60 patients with RA and 28 healthy control subjects was quantified by our highly sensitive enzyme-linked immunoad sorbent assay, and its relationship to clinical and laboratory variables was analyzed . Synovial specimens were obtained from 1 RA and 1 OA patients undergoing synovectomy.

体内实验：用酶联免疫吸附方法检测了60例RA和28例健康对照血清中的VEGF值，并检测其他活动性指标及血清中特异性自身抗体：抗核周因子抗体、抗角蛋白抗体、抗环瓜氨酸肽抗体，分析它们之间的关系。

Methods Serum transferrin of 100 patients with different types of hepatitis were determined with immuno turbidimetry and compared with prothrombin time, serum bilirubin and serum cholinesterase.

采用免疫比浊法检测100例不同临床类型病毒性肝炎病人的TF，同时检测凝血酶原时间、血清总胆红素、血清胆碱酯酶。

To study the changes between SOD activity and LPO content in serum of the patients with hepatitis B virus, the methods of xanthine oxidase and thiobarbituric acid were applied to this study.

为了研究乙型肝炎患者血清中超氧化物歧化酶活性水平、过氧化脂质含量及其变化规律，应用黄嘌吟氧化酶法和硫代巴比妥酸法测定了53例乙肝患者血清中SOD与LPO含量，其中急性乙型肝炎 35例，慢性活动性乙型肝炎 18例。

Methods Serum containing achyranthes root was prepared, human mesenchymal stem cells were separated and amplified, MTT assay was used to access the effects of serum containing achyranthes root on human mesenchymal stem cells in vitro, alkaline phosphatase and osteocalcin were assayed to reflect the variations of stem cell differentiation, and the expression of osteogenic transcription factors with RT-PCR.

制备牛膝含药血清，分离与扩增人间充质干细胞，MTT法检测牛膝含药血清对体外培养的间充质干细胞增殖作用的影响，检测碱性磷酸酶与骨钙素以反映细胞分化水平的改变，RT-PCR检测成骨相关转录因子的表达。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。