血清酶

The concentrations of BAFF were detected in 42 patients with ITP before and after treatment and in 40 patients to be operated selectively by ELISA.

采用双抗体夹心酶联免疫吸附试验定量方法，检测实验组治疗前后血清中BAFF的水平，同时检测对照组血清中BAFF的水平，并进行分析比较。

Mthods: The serum levels of IL-15 were measured by a double-antibody sandwich enzyme-linked immunosorbent assay in 47 severe hepatitis B patients and 20 healthy subjects. Parameters of liver function including alanine aminotransferase and total bilirubin were detected by automatic biochemistry analyzer, meanwhile prothrombin activity were measured by automatic coagulator. In the same time, the hepatitis A, B, C, D and E virus markers were examined in all patients.

方法采用双抗体夹心酶联免疫吸附试验检测47例重型乙型肝炎患者和20例体检健康者血清IL-15的水平，并通过全自动生化仪检测血清丙氨酸转氨酶、总胆红素，全自动血液凝固仪检测凝血酶原活动度，同时采用ELISA方法检测甲、乙、丙、丁、戊型肝炎病原学。

Model group and the normal group were taken distilled water,rats in the Tangshukang group were taken Tanggukang powder, western medicine control group were taken Calcium Carbonateand alpha D3. once a day for 12 weeks. Ca,P, BALP,BGP in blood and bone miner density;bone mineral contents, osseous vitodynamics,the content of Ca and P in the cremains were tested respectively.The femur was taken and detected the expression of TGF-β_1imRNA , the duodenum was taken and detected expression of VDR mRNA and CaBp-D9K mRNA with situ hybridization.

分别给予糖疏康、碳酸钙阿法迪三和生理盐水灌胃给药。12周后取材，测体重、血糖、血清钙、磷、血清骨源性碱性磷酸酶、骨T-羧基谷氨酸蛋白、骨密度、骨矿含量、骨的生物力学、骨干重、灰重、骨钙、磷等指标、组织病理切片骨形态计量学指标测定、并进行维生素D受体mRNA、转移生长因子-β_1(TGF-β_1)mRNA、钙结合蛋白(CaBp-D9K)mRNA原位杂交。

According to experimental results and etiopathogenisis and pathology of osteoporosis , and analyzing review investigation on hepcidin、associations of bone metabolism and iron metabolism and relations of ferri ion and calcium ion,we can make conclusions as follows: 1、We applied single manmade intervention approach to induce osteoporosis model in this study, there was no other influential factor. In the termination of test , rats bone density and bone histological anatomy indicated that osteoporosis model was creditable,meanwhile hepatic functional enzyme and hepatic microtome section demonstrated that hepatic function had noci-influence induced by medicinal herbs resource. Accordingly, we could believe that hepatic hepcidin gene expression and serum hepcidin contents can represent internal iron metabolism changes at selected test time. 2、In this investigation, different findings between test group and control group indicated that:according to changes of hepcidin gene expression measured by RT-PCR and changes of serum hepcidin contents determined by ELISA kit , there were correlations between hepcidin and rat osteoporosis model induced by retinoic acid. 3、Under the condition of this investigation,there was a interactive hypothesis as follows: Hepcidin iron metabolism←→ferriion←→calcium ion←→osteoporosis.

根据本次实验结果，对骨质疏松的病因病理的认识及铁代谢与铁调素、骨代谢与铁代谢关系、铁离子与钙离子的关系系列研究的回顾，经分析得出如下结论：1、本研究骨质疏松模型采用单一人工干预方法，没有其他影响因素；实验结束时大鼠骨密度、骨组织学检查表明骨质疏松形成，同时肝脏功能酶和HE切片显示肝脏功能未受药源性产生明显损伤性影响；因此，同期不同时间组大鼠肝脏铁调素基因表达、血清铁调素含量可以代表体内铁代谢不同时间的变化。2、在本研究中，模型组与对照组研究结果的差异表明：铁调素基因RT-PCR变化、血清铁调素ELISA测定含量变化与维甲酸制作的大鼠骨质疏松模型形成有实验相关性。3、在本研究平台下，形成了一个互相影响的假设图：铁调素←→铁代谢←→铁离子←→钙离子骨质疏松。

Results The serum antibody level reached to 0.71±0.08-0.77±0.06 after genetic vaccine pCD-HCV1(100 μg/mouse) were inoculated into the mice(n=12) three or four times while blank vector pCD-SRα1 could not induce the mice(n=8) to generate antibody response in same way.After the antibody levels in mice(n=8) immunized by pCD-HCV1 had ascended to peak value(0.71), there was no trend of descending during the following 18 weeks of detection(0.68±0.06-0.75±0.07). Specific fragment of HCV cDNA identified by polymerase chain reaction from DNA extracted from the muscles of the mice after pCD-HCV1 had beed inoculated three months.

结果 基因疫苗pCD-HCV1(100 μg/只)3-4次接种小鼠后(n=12)，血清抗体水平达0.71±0.08-0.77±0.06，空载体pCD-SRα1免疫鼠血清抗体阴性；免疫鼠(n=8)抗体水平达高峰后(0.71)，持续检测18周未见下降趋势(0.68±0.06-0.75±0.07)；pCD-HCV1免疫小鼠3个月后，用聚合酶链反应仍可从肌肉组织中扩增出特异性HCV cDNA片段；免疫鼠PBMC对HCV合成肽CP9、基因重组抗原C、E1刺激后均出现增殖反应，刺激指数分别为4.07±1.58、3.88±0.70和3.69±1.30，与pCD-SRα1免疫鼠相比，差异有显著性(P.001)。

Objective To study the change of lipase expression in patients with chronic kidney disease .

目的探讨血清脂肪酶在慢性肾脏疾病患者血清中表达的变化。

Objective To study the change of lipase expression in patients with chronic kidney disease.

目的 探讨血清脂肪酶在慢性肾脏疾病患者血清中表达的变化。

METHODS: One hundred and fifty-one children with Kawasaki disease (111 cases with coronary artery damage and 40 cases without) and 60 healthy children were enrolled. The expression of MMP-2, MMP-9 and TIMP-1 was detected using ELISA, and the hs-CRP concentration was measured using the endpoint nephelometry.

选取151例川崎病患儿作为观察组（无冠状动脉损伤40例，有冠状动脉损伤111例），60例健康儿童作为对照组，采用酶联免疫吸附实验检测血清中MMP-2、MMP-9和TIMP-1含量，应用终点散射比浊法检测血清hs-CRP的含量。

Methods:Preparing pastille serum of Rehmanniae pill of Six Ingredients with low,medium and high doses and using 10% pastille serum with different doses to cultivate OS-732 cells.

制备低、中、高剂量的六味地黄丸含药血清。10%低、中、高剂量的六味地黄丸含药血清培养OS-732细胞，显微镜下观察细胞生长情况；进行细胞计数和绘制生长曲线；用MTT法检测细胞增殖；进行细胞碱性磷酸酶活性测定。

METHODS: A total of 129 gastric mucosa biopsies and its corresponding serum specimens were collected from patients with superficial gastritis (n=30), gastric ulcer or erosion (n=35), atrophic gastritis (29), and gastric cancer (n=35). The expression of PGC in the gastric mucosa was detected by immunohistochemistry, and the concentration of serum pepsinogen A and pepsinogen C were determined by enzyme linked immunosorbent assay.

129例胃黏膜活检组织及其血清标本（浅表性胃炎30例，胃黏膜糜烂溃疡35例，萎缩性胃炎29例，胃癌35例），采用免疫组织化学染色检测胃黏膜标本中PGC的表达情况；采用酶联免疫吸附实验检测血清PGA和PGC含量。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。