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Blood samples were processed within 3 h after phlebotomy and centrifuged at 1500g for 10 minutes, and serum was stored in aliquots at 80°C until analysis.

血液样本在抽血3小时之后进行处理,并在1500g的状态下离心旋转10分钟,血清以测定用量储存在- 80 °C的低温下,留待分析使用。

Objective To explore the effect of different anticoagulants on quantitative assays for HBV DNA using real-time PCR technique. Methods Peripheral blood samples were obtained from 40 patients infected with HBV. Serum and plasma samples from three kinds of anticoagulants such as heparin, trisodium citrate, and dipotassium EDTA were stored at -20℃.

目的 探讨用实时荧光定量PCR技术检测乙型肝炎病毒DNA拷贝时,不同抗凝剂对乙型肝炎病毒DNA定量检测的影响方法采集40例乙型肝炎患者血液,分别置于不含抗凝剂及含肝素钠、枸缘酸钠、EDTA-K2抗凝剂的无菌试管中,分离血清和血浆后于-20℃冰箱保存备用。

The results of blood biochemical parameters assay indicated that alanine aminotransferase, cholesterol and total protein have a fargoing change, especially aspartate aminotransferase.

似刺鳊鮈血清生化指标测定结果显示,谷丙转氨酶、胆固醇和总蛋白变化范围较大,且谷草转氨酶的水平较高,似刺鳊鮈雄鱼的血液生化指标相应值均显著低于雌鱼(P.05)。

The changes of correlation coefficients and regression equation between the serum testosterone levels and hematocyte parameters did not show regularity and characteristics.

血清睾酮含量和血液细胞的相关系数和回归方程无明显的规律性和特征性。

In a prospective trial, they evaluated this hypothesis using serum samples obtained from 31 patients who underwent allogeneic transplantation using human leukocyte antigen-identical siblings as donors. All patients in the cohort received 1 to 3 pretransplant chemotherapy cycles to induce uniform host lymphopenia, which was followed by fludarabine-based reduced-intensity conditioning. Cyclosporine methotrexate was administered as prophylaxis against the development of GVHD.

在一项前瞻性研究中,他们透过测量31位接受异体移植血液干细胞移植病患血清来评估这项假设,这些病患都是接受人类白血球抗原比对相符同属作为捐赠者,他们都接受移植前化学治疗1至3个周期,以诱发宿主淋巴球数目低下,接著以fludarabine为主的低密度调节化学治疗,且投予cyclosporine与methotrexate用于预防GVHD的发生。

In a prospective trial, they evaluated this hypothesis using serum samples obtained from 31 patients who underwent allogeneic transplantation using human leukocyte antigen-identical siblings as donors. All patients in the cohort received 1 to 3 pretransplant chemotherapy cycles to induce uniform host lymphopenia, which was followed by fludarabine-based reduced-intensity conditioning. Cyclosporine methotrexate was administered as prophylaxis against the development of GVHD.

在一项前瞻性研究中,他们透过测量31位接受异体移植血液干细胞移植病患血清来评估这项假设,这些病患都是接受人类白血球抗原比对相符同属作为捐赠者,他们都接受移植前化学治疗1至3个周期,以诱发宿主淋巴球数目低下,接著以fludarabine为主的低密度调节化学治疗,且投予cyclosporine与methotrexate用於预防GVHD的发生。

The number and proliferative responses of T and B lymphocytes to ConA or phorbol myristate acetate,contents of IgG,IgM and IgA in peripheral blood of 14-days-old chickens secondarily infected with E.necatrix were elevated diffe-rent extent compared with that of the control.

结果显示,毒害艾美球虫二次感染雏鸡外周血液T、B淋巴细胞数量及其对ConA或PMA的增殖反应和血清IgG、IgM、IgA免疫球蛋白含量均不同程度地高于未感染毒害艾美球虫的对照组雏鸡。

A acute partial obstructive hepatocholangitis model by selective ligation and injection of E coli into left hepatic bile duct was successfully founded in rat. Using parameters including mortality, mitochondrial glutamic oxalacetic transaminase and ornithine carbamoytransferase activity, pathological observation and blood culture of bacteria, we evaluated the model.

作者采用选择性左肝管穿刺注菌法成功地建立了Wistar大鼠急性部分肝胆管梗阻感染的脓毒症模型,并从胆管系统形态学、累计病死率、血清线粒体谷草转氨酶和鸟氨酸氨基转移酶活性、多器官大体病理观察以及血液细菌培养几方面对该模型的科学性、重复性进行了评估。

Result:It was showed that Zhechong not only lowered markedly whole blood viscosity,plasmatic viscosity and packed cell volume but also promoted red cell immune function,which may be:①RBC C3b receptor rosette rate(RCR1R) could be enhanced,②RBC immune complexes rosette rate could be reduced,③the serum rosette forming enhancing rate of RBC C3b receptor could be increased,④the serum rosette forming inhibitory rate of RBC C3b receptor could be decreased.Moreover,there were the inverse correlation between blood viscositv and RCR1R.

结果:虫能明显降低瘀血大鼠的全血比粘度、血浆比粘度和红细胞压积;提高瘀血大鼠红细胞C3b受体花环率,使红细胞免疫复合物花环率下降,血清红细胞免疫粘附促进因子活性增强,而抑制因子减弱;血液粘度与红细胞C3b受体花环率的相关分析呈显著负相关。

Methods Detected IL-2, IL-5, IL-10 and EOS of 60 cases in patients with eczema and 40 cases health controls by ABC-ELISA and detect counting method.Analyzed the data with the SPSS 11.5 software. Results The IL-2 in patients with eczema wase lower than that of the health groups.

采用双抗夹心法和直接计数法分别检测湿疹患者60例和正常健康体检者40例的血清IL-2,IL-5,IL-10和血液中EOS的水平,应用SPSS11.5统计软件包对结果作统计学分析。

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