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血清脂酶

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An indirect competitive enzyme-linked immunosorbent assay was established for detecting vitellogenin in the whole body homogenate of male swordtail fish.This technique was developed with antiserum of lipovitellinresistance as antibody and Vtg as antigen.

以卵黄脂磷蛋白(lipovitellin,Lv)抗血清为抗体,以纯化的卵黄蛋白原(vitellogenin,Vtg)为抗原,建立了间接酶联免疫吸附反应方法检测剑尾鱼雄鱼整体匀浆液中ρ。

Reproducibility, recovery rate, filate range, interference of haematolysis and lipaemia were evaluated by NCCLC system, and then the results were compared.

用钒酸氧化法、改良J—G法和胆红素氧化酶法测定血清中的总胆红素,按NCCLC评价方案系统评价其重复性、回收率、线形范围及溶血、脂血对胆红素测定的干扰影响,然后对测定结果进行比较。

The test measured the inhibition of binding between a labeled,monoclonal antibody 3-47-0 and Salmonella pullorum lipopolysaccharide.

该方法基于待检血清样品抑制酶标单抗与脂多糖的结合,以检测鸡白痢和鸡伤寒特异性抗体。

The results showed as:(1)Gegen decreases fasting insulin and insulin resistance index and increases insulin sensitive index of rats of insulin resistance induced by dexamethasone significantly;(2)Gegen can improve glucose tolerance of rats of insulin resistance but not to alloxan indued diabetes mice;(3)Gegen increases glucose intake of 3T3-L1 adipocytes and improves the insulin resistance of 3T3-L1 adipocytes induced by dexamethasone significantly;(4)Gegen can activate peroxisome proliferator- activated receptor gamma, make it bind to PPAR response elements;(5)Gegen can up-regulate mRNA expression of PPAR v ,aP2 and adiponectin.

结果表明:①葛根显著降低地塞米松诱导的胰岛素抵抗大鼠血清胰岛素水平和胰岛素抵抗指数,升高胰岛素敏感指数,改善大鼠的胰岛素抵抗;②葛根显著改善胰岛素抵抗大鼠的糖耐量,但对四氧嘧啶糖尿病小鼠的糖耐量没有明显的作用;③葛根显著增加胰岛素抵抗3T3-L1脂肪细胞对葡萄糖的摄取,改善地塞米松诱导的3T3-L1脂肪细胞的胰岛素抵抗;④葛根能促进HepG2肝癌细胞过氧化物酶体增殖物激活受体γ与靶基因的特异DNA序列结合,对PPARγ及其下游基因的转录具有激活作用;⑤葛根能够促进PPARγ及其下游基因脂肪酸结合蛋白(aP2)、脂联素mRNA的表达。

Study objective To search the changing of serum Lipid Peroxides content and Glutathione Peroxidase activity in some central nervous system diseases.

研究目的探讨某些中枢神经系统疾病血清过氧化脂质和谷胱甘肽过氧化物酶水平的变化。

Aim To develop a sandwich ELISA for measuring serum β2-glycoprotein I-lipoprotein complex (β2-GPI-Lp concentration, and to explore the existence of serum complexes β2-GPI-Lp and its clinical value for IgA nephropathy. Methods ELISA was established using rabbit anti-human β2-GPI antibody as the capture antibody, and quantitating with polyclonal antibody against apolipoprotein enzyme conjugate.

目的 建立检测人血清β2-糖蛋白I-脂蛋白复合物的酶联免疫吸附法,探讨该复合物存在于人血循环中的可能性,并通过对IgA肾病患者的检测分析该复合物的临床诊断价值。

Lipopolysaccharide ; Chlamys farreri ; Serum ; Haemocytes ; Activity of enzyme

脂多糖;栉孔扇贝;血清;血细胞;酶活力

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