血清脂酶

As the body response for the trauma, the adipose tissues as blood serum behavior in the wounded tissue were suffered mobilization. At the same time, the ectogenesis fat tissue entranced blood circulation. As the result of hemodynamics alteration, there were blood platelet and erythrocyte and leucocytes and lipid granules collecting on the surface of these lipid droplets. The volumes of these lipid droplets were enlargement. These lipid droplets were changed to fat embolus by the promotion of coagulation and fibrin deposition intra-blood vessel.

现代医学理论认为，外伤后组织存留的血清状态的脂肪组织受到动员，同时，损伤部位的血管外源性脂肪进入血循环，而创伤引起的机体反应使血液动力学发生改变，血小板、红细胞、白细胞及血脂乳化不稳定所析出的脂质颗粒等，均可聚集于以上脂滴的表面，使之体积增大，加之组织凝血活化酶的释放，促使血管内凝血，纤维蛋白沉积的发生，从而形成脂肪栓子，导致脂肪栓塞。

Methods: The objects were all the works who were on the job or retired, the lipids kevel of TG.TC.HDL-C was measured by EIA.

检测对象全部为我市各单位在职及退休职工，用酶法测定甘油三脂、血清胆固醇、高密度脂蛋白胆固醇。

The apoB gene polymorphism on Xba I site of 53 6 unrelated Chinese Han people,aged 25～64 years,was analyzed using PCR and Re striction Fragment Length Polymorphismmethod;serum apoA I,apoB,total chole s terol,triglyceride,HDL cholesterolwere measured,and LDL choleste roland non-HDL cholesterolwere calculated.

采用聚 合酶链反应结合限制性片段长度多态性分析的方法，分析536名25～64岁的无血缘关系的汉族人的apoB基因Xba I位点多态性，并测定其血清apoA I、apoB、总胆固醇、甘油三酯、高密度脂蛋白胆固醇，并计算低密度脂蛋白胆固醇LDL-C）及非高密度脂蛋白胆固醇。

To study the changes between SOD activity and LPO content in serum of the patients with hepatitis B virus, the methods of xanthine oxidase and thiobarbituric acid were applied to this study.

为了研究乙型肝炎患者血清中超氧化物歧化酶活性水平、过氧化脂质含量及其变化规律，应用黄嘌吟氧化酶法和硫代巴比妥酸法测定了53例乙肝患者血清中SOD与LPO含量，其中急性乙型肝炎 35例，慢性活动性乙型肝炎 18例。

The experiment two: enzyme preparation significantly improved average daily gainand feed conversion ratio (P＜0.05). Enzyme preparation significantly increased energymetabolizability and digestibility of crude fiber, crude protein and neutral detergent fiber,but had no remarkable effect on digestibility of dry matter, crude fat and acid detergentfiber. Enzyme preparation significantly decreased the relative viscosity of duodenal andjejunal digesta. The pH of intestine had no noticed difference in all groups. Enzymepreparation significantly decreased relative weight of gizzard, proventficulus, duodenum,jejunum and ileum. Enzyme preparation significantly increased villus size of duodenumand jejunum, and villus to crypt ratio of duodenum and ileum significantly increased too.Enzyme preparation considerably decreased ileal crypt height (P＜0.05), and didn"t affectthickness of intestinal wall. Supplementing enzyme preparation, the serum glucose, totalprotein and alanine aminotransferase, but enzyme preparation hadn"t noticed influenceupon uric acid, total cholesterol, triglyceride and high-density lipoproteins. Enzymepreparation significantly increased insulin, triiodothyronine and insulin-like growthfactor-Ⅰ. Adding enzyme preparation, the percentage of thyroid stimulating hormone andgrowth hormone in the serum increased 16.44%, 19.18% and 18.84%, 21.74%respectively, and the percentage of glucagon and thyroxine decreased 12.07%, 14.36% and 13.79%, 15.40%, but failed to reach statistical significance (P＞0.05). Enzymepreparation significantly increased (P＜0.05) the trypsin and amylase activity of duodenaland jejunal digesta, but enzyme preparation didnt affect significantly (P＞0.05) theintestinal lipase activity and pancreatic digestive enzyme. Enzyme preparation had nosignificant effect on caecal microbial population.

试验二：酶制剂显著提高平均日增重和饲料转化率(P＜0.05)；酶制剂显著提高能量代谢率及粗纤维、粗蛋白、中性洗涤纤维消化率(P＜0.05)，而对干物质、粗脂肪、酸性洗涤纤维消化率影响不显著；酶制剂显著降低十二指肠和空肠食糜相对粘度(P＜0.05)；添加酶制剂对肠道pH影响不显著；酶制剂显著降低肌胃、腺胃、十二指肠、空肠、回肠相对重(P＜0.05)，显著提高十二指肠和空肠绒毛高度，显著增加十二指肠和回肠绒毛高度／隐窝深度，降低回肠隐窝深度(P＜0.05)，对肠壁厚度影响不显著；酶制剂显著提高血清葡萄糖、总蛋白和谷丙转氨酶浓度(P＜0.05)，对尿酸、总胆固醇、甘油三酯及高密度脂蛋白浓度影响不显著，显著提高胰岛素、T_3、IGF-Ⅰ水平，添加酶制剂后，促甲状腺激素、生长激素分别提高16.44％、19.18％和18.84％、21.74％，胰高血糖素和T_4分别降低12.07％、14.36％和13.79％、15.40％，但差异不显著；酶制剂对胰腺消化酶活性影响不显著，显著增加十二指肠和空肠胰蛋白酶、淀粉酶活性，对小肠脂肪酶活性影响不显著；酶制剂对盲肠微生物菌落数影响不显著。

To study the changes between SOD activity and LPO content in serum of the patients with cholecystolithiasis before and after operation, the methods of xanthine oxidase and thiobarbituric acid were applied to this study.

为研究胆囊结石患者血清中超氧化物岐化酶活性水平、过氧化脂质含量及其变化规律，应用黄嘌呤氧化酶法和硫代巴比妥酸法测定了48例胆囊结石患者手术前后血清中SOD活性和LPO含量。

A method for determination of Secoisolariciresonol diglucoside, secoisolariciresinol, enterolactone and enterodiol in serum by LC/MS/MS was established. In this method,β-Glucuronidase was used to hydrolyze the serum samples, the electrospray ionization sourse and negative ion MRM scan were employed.

本实验用β葡萄糖醛酸苷酶酶解血清样品，采用电喷雾离子源，负离子MRM扫描，建立了血清中司可异罗叶松甘油二酯、司可异罗叶松酯素、肠内二醇、肠内脂的IC/MS/MS分析方法。

Objective : To detect the serum level of triglyceride、total cholesterol、low-density-lipoprotein-cholesterol、plasma viscosity,fibrinogen 、the level of plasma tissue plasminogen activator,plasminogen activator inhibitor-1(PAI-1) and calculate the score of nervous impairment of acute ischemia apoplexy patients ; so as to compre- hend changes of lipid metabolism、hemorheology and fibrinolysis function of AIA patients and it relation of the condition degree, and to investigate relationship between the above-mentioned microcosmic indexes and phlegm blood stasis syndrome.

目的：通过观察急性缺血性中风患者血清甘油三酯、胆固醇、低密度脂蛋白、血浆粘度、纤维蛋白原、血浆组织纤溶酶原激活物及其抑制剂-1（PAI-1）和评定神经功能缺损程度积分以了解急性缺血性中风患者脂质代谢、血液流变性及纤溶系统功能的变化及其与病情程度的关系，并探讨上述微观指标与痰瘀证的关系。

It was suggested that the pharmacodynamics and therapeutic function of Qizhi complex prescription to fatty liver were: 1. Adjust lipometabolism: reducing TC and TG; 2. Decreasing fatty degeneration of liver cell; 3. Promoting the convalescence of hepatocellular function; 4. Anti-lipid peroxide: increasing the activation of peroxidase, decreasing the content of products of lipid peroxide. 5. Restraining Kupffer cell function of fatty liver rat; 6. Improving fluidity of hepatocellular microsome and mitochondria.

芪枳合剂治疗脂肪肝的效应及药效学机制为： 1、调整脂质代谢：降低血清甘油三脂和胆固醇； 2、减轻肝细胞脂肪变性； 3、促进肝细胞功能恢复，有效治疗脂肪性肝炎； 4、抗脂质过氧化：提高抗氧化酶活性、降低脂质过氧化产物含量； 5、抑制脂肪肝大鼠Kupffer细胞功能； 6、改善肝细胞微粒体、线粒体膜流动性。

objective:to investigate the influence of soybean isoflavones on the antioxidative effect and improving the ability of study and memory in climacteric rats.methods:forty-eight female sd climacteric rats were randomly divided into 4 groups with one control group and the other low,medium and high dose groups,which were given soybean isoflavones 0 mg/kg.bw,42 mg/kg.bw,83 mg/kg.bw and 249 mg/kg.bw by superalimentation daily for 60 days respectively.the levels of sod,gsh-px and mda in serum were measured,and the ability of study and memory in each group was deterimined by adopting the law of diving platform.results:the content of serum mda in medium and high dose groups was lower than that in control group.the activity of serum gsh-px im medium and high dose groups was significantly increased,so was the activity of serum sod in low,medium and high dose groups.conclusion:soybean isoflavones do have the obvious effect of antioxidation,and improve the ability of study and memory effectively in female sd climacteric rats.

目的：观察大豆异黄酮对更年期雌性sd大鼠抗氧化作用及学习记忆的影响。方法：选用健康更年期雌性sd大鼠，随机分成空白对照组和低、中、高剂量4组。空白对照组给予蒸馏水，实验组每日分别灌服42、83、249 mg/kg剂量的大豆异黄酮，连续60d。第60d取血测定血清过氧化脂质丙二醛含量、超氧化物歧化酶活力、谷胱甘肽过氧化物酶活力，用跳台实验方法测定大鼠学习记忆能力。结果：与空白对照组比较，中、高剂量组大鼠血清mda含量显著降低，低、中、高剂量组血清sod活力明显升高，中、高剂量组血清gsh-px活力明显升高。结论：大豆异黄酮具有明显的抗氧化作用，并有效提高更年期雌性sd大鼠的学习记忆能力。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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