血清肝炎

Objective 64 sera with serologically negative nonA-E were tested for HEV RNA and their serological characteristics were studied.

目的 探讨戊型肝炎病毒患者血清学特征。

Objective:To investigate the epidemiologic and clinical features of seronegative liver diseases and guide diagnosis and treatment.

目的：探讨肝炎病毒血清学标志阴性肝病患者流行病学和临床特点，指导临床诊断和治疗。

We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术，分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因，以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，分别自43例、51例慢性HBV感染患者血清中扩增出目的片段，构建质粒Teasy-BCP和Teasy-pre S2，酶切鉴定后，采用聚丙烯酰胺凝胶电泳技术展示缺失突变，再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

Results 20 UCB samples from the newborns whose mothers were triply positive for HBsAg, HBeAg, and HbcAb were detected using quantitative detection of HBV-DNA.

结果：HBV血清学标志物检测结果为大三阳，即HBsAg、HBeAg和HBcAb的乙型病毒性肝炎产妇。

Objective: To investigate the effect of anxiety and depression on HBV DNA, HBeAg and ALT in patients with chronic hepatitis B.

目的：探讨焦虑抑郁状态对慢性乙型肝炎患者血清HBV DNA、HBeAg及ALT的影响。

Results The re were different degrees of liver inflammation activity and ALT level in the patients with CHB.

血清生化是临床检测肝炎活动的最常用的方法，具有方便易行，创伤小，患者容易接受，可重复性检查等优点。

The destructive effect on HBsAg in serum was potentiated when chlorhexidine was added to the ethanol.

试验用正常人血清，取自献血员，肝炎标志物均为阴性。

To explore the level of HBV DNA after plasma exchange in patients with severe hepatitis and its prognoses.

探讨经血浆置换治疗重型乙型病毒性肝炎后，患者血清中HBV DNA含量的变化对预后的影响。

IL-12 and TNF participates in the activity process of viral hepatitis.Levels of serum IL-12 and TNF in early stage may reflect the severity of viral hepatitis and be helpful to prognosticating the disease.

结论IL-12及TNF参与了病毒性肝炎的免疫病理过程，早期检测血清IL-12与TNF水平，可判断病情的严重程度及预后。

More sensitive and reliable methods of quantitating HCV RNA in serum are needed.

更敏感和可靠的方法quantitating血清中丙型肝炎病毒RNA是必要的。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。