血清疫苗

Although IgG in serum not expected to increase, and the hemagglutination inhibition test also showed that there was insufficient protection of the effectiveness of the antibody.

动物实验显示褐藻胶包覆之疫苗在第二次免疫后3周可以诱发肠道sIgA的产生，虽然在血清中IgG没有预期中的提高，且血球凝集抑制实验也显示没有足够的抗体保护效力。

To obtain the African horse sickness virus VP7 protein, which is used to preparing serological diagnostic reagent and constructing AHS new generation vaccine, the prokaryotic expression is used to express VP7 protein.

为获得非洲马瘟病毒VP7蛋白，以用于制备AHS血清学诊断试剂并为AHS新型疫苗的构建奠定基础，笔者采用原核表达系统表达VP7蛋白。

Finally, HBsAg-pulsed DCs were administered, intradermally to six HB vaccine nonresponders and the levels of antibody to HBsAg in the sera were assessed.

在6位乙肝疫苗无应答者行负载的HBsAg血树突状细胞皮下注射给药，评估其血清HBsAb的水平。

Finally, HBsAg-pulsed DCs were administered, intradermally to six HB accine nonresponders and the leels of antibody to HBsAg in the sera were assessed.

在6位乙肝疫苗无应答者行负载的HBsAg血树突状细胞皮下注射给药，评估其血清HBsAb的水平。

Objective To analyze humoral immune response to nucleic acid vaccines (VR1012/TPA/HG-MSP1-17 for intracellular expression or VR1012/HG-MSP1-17 for secretion) which contain Plasmodium falciparum Merozoite surface protein 1(MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL-1 and TT and to monitor the effect of DNA-immune sera on parasite inhibition.

摘 要：目的检测以恶性疟原虫裂殖子表面蛋白1的17区片段基因为基础的复合核酸疫苗(分泌性的VR1012/TPA/HG-MSP1-17和非分泌性的VR1012/HG-MSP1-17)诱导小鼠的体液免疫反应和免疫血清对疟原虫生长的抑制能力。

The recombinant baculovirus was transfected into sf-9 cells by CellFectin reagent. SDS-PAGE and Western-blot analysis showed that the expressed protein was 37ku in molecular mass and exhibited specific immunological activity. These results provide foundation for development of a subunit vaccine with the expressed σC protein against Muscovy duck reovirus infection.

在脂质体介导下将rBacmid-σC转染sf-9昆虫细胞，获得重组杆状病毒rBV-σC；SDS-PAGE和Western-blot分析表明，在sf-9细胞中表达了分子质量约37ku的σC蛋白，该蛋白能与原核表达的σC蛋白免疫小鼠血清发生特异性免疫反应，这为以表达的σC蛋白为抗原的亚单位疫苗的研制奠定了基础。

Methods BALB/c mice were inoculated muscularly with HIV-2 gag recombinant DNA vaccine, or recombinant fowlpox virus, or both.

大量制备并纯化HIV-2 gag重组DNA疫苗和重组鸡痘病毒，以肌肉注射的方式免疫BALB／c小鼠，ELISA法检测小鼠血清HIV-2抗体，流式细胞仪测定CD4^＋、CD8^＋T淋巴细胞亚类数量，乳酸脱氢酶释放法检测脾CTL对HIV-2靶细胞的杀伤活性。

BALB/c mice were co-inoculated muscularly by this plasmid and the nucleic acid vaccine plasmid pVAXGE expressing the HIV-1 (Human immunodeficiency virus type I) gag-gp120 protein. The level of serum antibodies after immunization showed that the specific antibodies against HIV-1 appeared at the second week and raised to the peak level at the sixth week for the co-immunization group.

将它与表达I型人免疫缺陷病毒(Human immunodeficiency virus 1， HIV-1) gag-gp120的核酸疫苗质粒pVAXGE共同肌肉注射BALB/c小鼠，免疫3次后，以ELISA法检测免疫小鼠血清中抗HIV-1抗体水平，结果显示联合免疫组小鼠在免疫2周后已有抗体产生，6周后进入高峰。

Results: The most of RFIs were happened in spring and summer, the incidence rate of measles was 24.80%, the highest incidence rate was march, nettle rash was 20.08%, the highest incidence rate was march and april, the most of RFIs cases were no history of vaccine inoculates.

结果：RFIs发病以春夏季为主，以3~6月份为高峰，主要在年龄0~4岁组，在254例RFIs病人的血清中，麻疹IgM阳性检出率为24.80%，以3月份最多；风疹为20.08%，以3、4月份为高峰，大部分RFIs病例无或不详疫苗接种史。

All of them were bled prior to and 3 years after entering this programme and measured the antibody against epidemic parotitis and rubella by enzyme-linked immunosorbant assay.

在疫苗接种前和三年后各抽血一次，然后用 EL ISA方法检测接种前后血清中特异性抗体的浓度，并观察接种组和对照组这两种传染病的发病情况。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷