血清疫苗

Results The serum antibody level reached to 0.71±0.08-0.77±0.06 after genetic vaccine pCD-HCV1(100 μg/mouse) were inoculated into the mice(n=12) three or four times while blank vector pCD-SRα1 could not induce the mice(n=8) to generate antibody response in same way.After the antibody levels in mice(n=8) immunized by pCD-HCV1 had ascended to peak value(0.71), there was no trend of descending during the following 18 weeks of detection(0.68±0.06-0.75±0.07). Specific fragment of HCV cDNA identified by polymerase chain reaction from DNA extracted from the muscles of the mice after pCD-HCV1 had beed inoculated three months.

结果 基因疫苗pCD-HCV1(100 μg/只)3-4次接种小鼠后(n=12)，血清抗体水平达0.71±0.08-0.77±0.06，空载体pCD-SRα1免疫鼠血清抗体阴性；免疫鼠(n=8)抗体水平达高峰后(0.71)，持续检测18周未见下降趋势(0.68±0.06-0.75±0.07)；pCD-HCV1免疫小鼠3个月后，用聚合酶链反应仍可从肌肉组织中扩增出特异性HCV cDNA片段；免疫鼠PBMC对HCV合成肽CP9、基因重组抗原C、E1刺激后均出现增殖反应，刺激指数分别为4.07±1.58、3.88±0.70和3.69±1.30，与pCD-SRα1免疫鼠相比，差异有显著性(P.001)。

Facts as increasing number of dog raising, having not been injected with rabies vaccine and antirabies serum timely or full course after exposure, unstrictly controlling the quality of rabies vaccine as well as lacking good cooperation in different official department on rabies control might serve as factors responsible for the recurrent increase of human rabies.

养犬数增加、没有及时全程使用狂犬病疫苗和抗血清、疫苗质量把关不严以及缺乏不同部门间的合作是流行回升的因素。

S2 and M5 vaccines broadly used in preventing caprine and ovine brucellosis were vaccinated to the sheep.

将国内广泛用于预防羊布病的S2和M5疫苗免疫绵羊，然后定期采血进行细菌分离和血清学检测，并比较血清学方法的检测结果。

Healthy dogs were inoculated with rabies vaccine and antibody titre was measured by ELISA and FAVN method from the 14th day. The immunized dogs were challenged with rabies virus BD06 strain.

将40只犬免疫兽用狂犬病疫苗后，第14 d静脉采血分离血清，分别采用ELISA法和国际贸易指定试验FAVN法检测血清样品，试验犬免疫第21 d攻击狂犬病毒BD06株。

Twenty five patients with gastritic carcinoma were vaccined with dendritic cells pulsed with tumor antigen and T cell subsets of peripheral blood, Th1 type and Th2 type (IL-4) cytokines of these patients serum were detected at different time point.

第三部分，从外周血诱导制备DCs疫苗，皮下注射治疗25例胃癌患者，初步观察DCs疫苗治疗前后胃癌患者迟发性超敏反应，T淋巴细胞亚群，NK细胞，外周血清IFN-γ、IL-4的水平及外周血PBMC在自体肿瘤抗原刺激下分泌IFN-γ、IL-4的能力。

Incidence of infection have not yet been possible under the assumption that healthy dogs and dogs with disease threats should immediately distemper hyperimmune serum for passive immunization or the use of children with measles Yi Miaozuo emergency vaccination Dai stable disease, then injected with canine distemper Yi Miao .

对尚未发病有感染可能的假定健康犬及受疫情威胁的犬，应立即用犬瘟热高免血清进行被动免疫或用小儿麻疹疫苗做紧急预防注射，待疫情稳定后，再注射犬瘟热疫苗。

Methods:Prepared multilaminar liposome vaccine by film evaporaqtion combined with lyophilization; Immunized BALB/c mice with the obtained liposomeencapsulated influenza split vaccine and the routine influenza split vaccine, serum antibodies were assayed on weeks 213 by the haemagglutinationinhibition test and ELISA, the HI test for the special antiHA antigen and the ELISA test for the IgG. Protective immunity against intranasal virus challenge was determined at 12 weeks postvaccination.

采用薄膜法与冷冻干燥法相结合的方法制得多层脂质体；用制得的脂质体流感疫苗和普通疫苗分别免疫小鼠，用血凝抑制实验和酶联免疫吸附试验对免后2～13周的小鼠血清的抗体水平进行检测，HI检测其特异性抗体，ELISA检测IgG抗体水平并对小鼠的保护效率进行检测。

Up to now it was the first time that the core-coated biodegradable microspheres containing Interferon α-2a composed of calcium alginate nanoparticles surrounded by the PELA coat was reported.

动物体液免疫检测表明与常规液体疫苗制剂相比，单剂注射PELA乙肝疫苗微球制剂更能有效诱导全身免疫应答，血清中产生的IgG1抗体滴度最高。

Methods Live attenuated rubella vaccine virus strain RA27/3 was inoculated to the monolayer of human diploid MRC-5 cells at an appropriate MOI and incubated in serum-and protein-free CDM culture medium. The virus liquid was harvested and, after addition of polyvinylpyrrolidone as a stabilizer and protective agent, lyophilized. Perform overall control tests on the prepared vaccine.

将风疹减毒活疫苗毒种RA27/3株按适当比例接种单层人二倍体细胞MRC-5株，使用无血清、无蛋白的CDM培养基培养病毒，收获病毒液，加入聚乙烯吡咯烷酮作为病毒稳定剂，制备疫苗原液，加入冻干保护剂，制备5批冻干疫苗，进行各项检定。

Methods Live at-tenuated rubella vaccine virus strain RA27 /3 was inoculated to the monolayer of human diploid MRC-5 cells at an appropriate MOI and incubated in serum-and protein-free CDM culture medium.

方法将风疹减毒活疫苗毒种RA27/3株按适当比例接种单层人二倍体细胞MRC-5株，使用无血清、无蛋白的CDM培养基培养病毒，收获病毒液，加入聚乙烯吡咯烷酮作为病毒稳定剂，制备疫苗原液，加入冻干保护剂，制备5批冻干疫苗，进行各项检定。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。