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The authers give a brief account of the present situation and prospects for the application of immunochemical methods to agricultural sciences, inculding identification of species, diagnosis of animal and plant diseases, quantitative and locative analysis of substances of biological activity in living things, determinat...

本文介绍了农业科学研究中,在物种鉴定、快速诊断和检疫、生物活性物质的测定、性状标记物和次生代谢物的测定、食品和环境中残毒的检测、生物物质的定位等方面血清学技术的应用现状和前景。

Objective The sensitivity and specificity of four kinds methods detecting Treponema pallium antibody were compared and clinical application values, which were analyzed in the Early Syphillis.

摘 要]目的:评价在梅毒早期诊断中,四种梅毒血清学检测方法的比较及在临床诊断中应用价值。

This paper summarizes the research progresses of APP by etiology, serology and molecular biology.

目前,国内外对该病病原学特性、血清学检测技术以及分子生物学技术的诊断方法研究比较多,文章对该病的病原学和诊断方法研究进展进行了综述。

Objective To study the clinical application of Toluidine Red Unheated Serum Test and Treponemal Pallidum Particle Agglutination for diagnosis and treatment of syphilis.

目的探讨梅毒血清学试验甲苯胺红不加热血清试验与梅毒螺旋体抗体明胶颗粒凝集试验在梅毒诊断及治疗中的作用。

Objective To explore the clinical value of the titer of serum HP antibody in patients with gastric diseases.Methods Serous HP antibody was detected with ELISA and HP was cultured,tested with urase,and stained with Gram method on smears which was observed microscopically and compared with proteus protein-enveloped test.Results ELISA showed high sensitivity and specificity.Conclusion Serous ELISA detection of HP antibody can be used widely in clinical diagnosis and epidemical screening without endoscopy and biopsy.

目的 探索胃部疾病患者血清中的幽门螺旋菌HP抗体滴度的临床价值方法应用酶联免疫吸附试验ELISA法检测HP抗体与HP培养,尿素酶试验和涂片革兰氏染色作镜下形态检查相比较,并同时与变形杆菌蛋白包被测定法作比较结果显示该法具有敏感特异性强的特点结论该法无需常规内镜检查,活检查HP而单纯用血清学检查,可广泛应用于临床诊断和流行病学人群普查

Sample collection: Two hundred and fifteen sera samples of normal population were collected from the peripheral blood of healthy blood donors of Shenyang, and the sera were separated by centrifugation after blood clotting; The patients sera were collected from the outpatients and inpatients of No. 1 hospital of China Medical University and the Second hospital of Jilin University. Syphilis was confirmed by clinical and serum test of TPHA and RPR; That of pemphigus vulgarism was confirmed not only by clinical, histopathological examination, but also by direct and indirect immunofluorescence test. DIF test on lesion or non-lesion skin showed immunoglobin and/or C3 deposits in the intercellular space of epidermis, and IIF test showed positive anti- intercellular antibody in patient serum using mouse's or rat's tongue or esophagus as substrate.

标本的收集:正常人的血清:采集沈阳市正常献血员外周静脉血共215份,待血凝后离心分离血清;患者的血清:来自中国医科大学一院及吉林大学二院门诊及住院部的病人,梅毒患者除了病史及临床表现外,全部经血清学TPHA及RPR确认;寻常性天疱疮患者除了临床及组织病理诊断外,还进行了直间接免疫荧光的检测,直接免诊断疫荧光:在皮损区及非皮损区的表皮细胞间显示IgG和/或C3补体沉积;间接免疫荧光用鼠或兔的舌或食管作为底物,在病人的血清中显示有肯定的细胞间抗体。

Three categories of cytokines, including Thl/Th2 cytokines, chemokines and adipocytokines, are involved in the physiopathological process of NASH. In order to elucidate the significances of cytokines in the pathogenesis, serological diagnosis, and treatment efficacy evaluation of HASH, we reviewed the documents on the relationship between cytokines and non-alcoholic steatohepatitis.

参与NASH病理生理过程的细胞因子归纳起来有3类,即Th1、Th2细胞因子和趋化因子及脂肪细胞因子,本文了回顾3类细胞因子与NASH发生、发展关系的最新研究进展,从而阐明细胞因子在NASH发病机制、血清学诊断和治疗效果评价等方面的意义。

Three categories of cytokines, including Th1/Th2 cytokines, chemokines and adipocytokines, are involved in the physiopathological process of NASH. In order to elucidate the significances of cytokines in the pathogenesis, serological diagnosis, and treatment efficacy evaluation of HASH, we reviewed the documents on the relationship between cytokines and non-alcoholic steatohepatitis.

参与NASH病理生理过程的细胞因子归纳起来有3类,即Th1、Th2细胞因子和趋化因子及脂肪细胞因子,本文了回顾3类细胞因子与NASH发生、发展关系的最新研究进展,从而阐明细胞因子在NASH发病机制、血清学诊断和治疗效果评价等方面的意义。

Historically, one limitation of the killed accines is that accinated birds cannot be differentiated serologically from naturally infected birds using the commonly aailable diagnostic tests.

但是经这种全灭活疫苗接种后的禽类与感染了禽流感病毒的禽类体内都会产生相同的抗体,通常的血清学诊断试验不能区分识别。

A dominating antigenic region located at the amino-terminus of Hantaan virus nucleocapsid protein.These recombinant truncated proteins can be used to diagnose the infection of HFRSV serologically.

汉滩病毒核蛋白的氨基端含有一主要抗原决定簇区;原核表达的截短核蛋白在HFRS的血清学诊断中具有一定的价值。

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